LOCUS       sequence-469645-        9235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of Chimera of D-region cCGNL1 in cCGN in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Paschoud S, Guillemot L, Citi S
  TITLE     Distinct domains of paracingulin are involved in its targeting to 
            the actin cytoskeleton and regulation of apical junction assembly.
  JOURNAL   J Biol Chem. 2012 Apr 13;287(16):13159-69. doi: 
            10.1074/jbc.M111.315622. Epub 2012 Feb 7.
  PUBMED    22315225
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.315622"; journalName: "J Biol Chem"; date: 
            "2012-04-13- 13"; volume: "287"; issue: "16"; pages: "13159-69"
COMMENT     Sequence Label: GFP-cCGN-cCGNL1_D-Myc
FEATURES             Location/Qualifiers
     source          1..9235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        261..590
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      441..576
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             599..1624
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             599..1624
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    1741..1822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1778..1797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1842..1860)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1928..2032
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2033..2101,2102..2893)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2251..2270)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3064..3652
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3553..3572
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3806..3823
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4203..4222
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    4221..4276
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     4322..4341
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     intron          4473..5045
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             complement(5091..5120)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(8298..9008,9009..9011,9012..9014))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8298..9008,9009..9011,9012..9014))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8340..8361)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8689..8708
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8948..8969
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9011..9020
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9120..9144)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9141..9179)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(9212..9230,9231..9235,1..18,19..37,38..60,61..79,
                     80..102,103..121,122..144,145..163,164..186,187..205,
                     206..228,229..247)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt
       61 tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc actgataggg
      121 agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt
      181 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg
      241 atagggagtg gtaaactcga gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
      301 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
      361 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
      421 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      481 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      541 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttccat
      601 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
      661 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
      721 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
      781 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
      841 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
      901 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
      961 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     1021 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     1081 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     1141 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     1201 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     1261 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     1321 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     1381 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     1441 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     1501 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     1561 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     1621 atagagttct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     1681 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     1741 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     1801 aataaagcat ttttttcact gcctcgagtg aagacgaaag ggcctcgtga tacgcctatt
     1861 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     1921 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1981 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2041 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2101 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2161 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2221 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     2281 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2341 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2401 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2461 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2521 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2581 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2641 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2701 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2761 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2821 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2881 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2941 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3001 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3061 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3121 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3181 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3241 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3301 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3361 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3421 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3481 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3541 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3601 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3661 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3721 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3781 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3841 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagtc gagggatctc
     3901 cataagagaa gagggacagc tatgactggg agtagtcagg agaggaggaa aaatctggct
     3961 agtaaaacat gtaaggaaaa ttttagggat gttaaagaaa aaaataacac aaaacaaaat
     4021 ataaaaaaaa tctaacctca agtcaaggct tttctatgga ataaggaatg gacagcaggg
     4081 ggctgtttca tatactgatg acctctttat agccaacctt tgttcatggc agccagcata
     4141 tgggcatatg ttgccaaact ctaaaccaaa tactcattct gatgttttaa atgatttgcc
     4201 ctcccatatg tccttccgag tgagagacac aaaaaattcc aacacactat tgcaatgaaa
     4261 ataaatttcc tttattagcc agaagtcaga tgctcaaggg gcttcatgat gtccccataa
     4321 tttttggcag agggaaaaag atctcagtgg tatttgtgag ccagggcatt ggccacacca
     4381 gccaccacct tctgataggc agcctgcacc tgaggagtga attctttgcc aaaatgatga
     4441 gacagcacaa caaccagcac gttgcccagg agctgtagga aagagaagaa ggcatgaaca
     4501 tggttagcag aggggcccgg tttggactca gagtatttta tcctcatctc aaacagtgta
     4561 tatcattgta accataaaga gaaaggcagg atgatgacca gggtgtagtt gtttctacca
     4621 ataagaatat ttccacgcca gccagaattt atatgcagaa atattctacc ttatcattta
     4681 attataacaa ttgttctcta aaactgtgct gaagtacaat ataatatacc ctgattgcct
     4741 tgaaaaaaaa gtgattagag aaagtactta caatctgaca aataaacaaa agtgaattta
     4801 aaaattcgtt acaaatgcaa gctaaagttt aacgaaaaag ttacagaaaa tgaaaagaaa
     4861 ataagaggag acaatggttg tcaacagagt agaaagtgaa agaaacaaaa ttatcatgag
     4921 ggtccatggt gatacaaggg acatcttccc attctaaaca acaccctgaa aactttgccc
     4981 cctccatata acatgaattt tacaatagcg aaaaagaaag aacaatcaag ggtccccaaa
     5041 ctcaccctga agttctcagc tctagagata tcgtcgaggt cgacctaatt cagatcctct
     5101 tctgagatga gtttttgttc atcgatacag gagctggtct gcaggttgct ctccgtaagc
     5161 agcgatgcaa tggaagaagg gtcgtagaca ctgtcaaact cctcatccga gctcagccct
     5221 tcacgctggg ctgactcagc agctgaacgg gaggctttgc gccaggagtc cttctctagg
     5281 gttttgatcc gggcctggag ctgttcattg acctcatgtt gctcttccag ctcacgctgg
     5341 gccttcttcc tcaggccatc cagtcgctca atctcctctt ctgcttcatc cacctgccgc
     5401 ttcaaagcct tcaccctcag gcttagctgg tccttctggt cattgacatg ctgccgctca
     5461 tcatcaattt ggatggacag ctctttaacc ctccgctcca gttttcggtt ggtggactga
     5521 aggaccgtct tctccctctc ttcagcctgc agccgctcct gcaactcctg attctgggat
     5581 tcaagctgag agaggctggc actgggcttc tggaagcctt ctgagctggc caatcggctc
     5641 ttcaagtcct tgttctgtct ctctagggag attttgtcac actccaggtc ctgtcgagca
     5701 gatctctcct gcatgagctc tgacctcagc tggtctacct ggtcccggcc acgagtcaca
     5761 cgttctgtca gcagctccac ggtactcctc tcctcatcta actctgcttc cagccgtgag
     5821 actttttcct caagaccctt cagctgccgg gccctgtcat cctgggagcg cttcttgttc
     5881 tctgcctctt gctccaggcc ctgcagtcgc tgggccagca gctctttatc cagccttgct
     5941 gtgtcccggt cagcctggga tgcctgcagg gcctgccgga gcctctgggt ctcatcctga
     6001 agccggctca gcccccctga gttcttctca gcctcagtgg cccattcctt ggcctgacgc
     6061 tgggcatccg ccacctcccg ccgggccttc tccttgtaat cctccagctg ggcctggagc
     6121 tgctgcagag cctgcttaga atcgttccca atctgctcca gctccttgtt gagctggcac
     6181 acagtcttgt ccagcaagcg cttctgttcc tccagctcag ccttgctccg ccggagcgcc
     6241 tcccgctgct tcccctcctc ctctagggcc cggttcagtg ctagcgcatt cacaagggcc
     6301 tcctctaatt gccggttttc tctctcgcat tttgtgagcc tttcctttgc cttgacctca
     6361 tcgtccttca tgtcagcaat ctgcctctgc agccgagcga cgctctgctc cagctcttcg
     6421 atgctttccc gcagcttctc attctcctcc tgcagagcct tcacgtgcat ttcagccccc
     6481 agctggctct gctctgaagt gttgctccga ctggccagga cctcgacgtt cttggttgct
     6541 tcttctacac tctccctcag ggcctgcagc tcggcgtcat actgctcctt cagcttatcc
     6601 atctccaggt cgtggctgga cacctcttcc ttcagggctc ccttcagggc ggtcagttca
     6661 cgctcccgct tcctcaagag gtcctcctgc tcctctttgg cgatgagcag atcctgaaga
     6721 tcctgttttg cctgtaagag ttcctcaatc agggctccct tttccctgtc ctcagatcgt
     6781 ttagcactgt ccaattcatc gtgcatttct gagagctggt cctggagatc cctgatctca
     6841 gtctggtgct gttcccgttc catcttcacc tggaacaatt cctccaggtt cttgcggagc
     6901 tccccttcac tctcttccag gcgctgctgt agcatggagt tctcttccac tttactatca
     6961 tgctggccct ggagctcctc caaatttgct ttcattctct ctctctcttc tttgatgttc
     7021 tgctgattct tgacttctag ttgaagctgt ctctgaagtt cagccacttc tgtggtcagc
     7081 ttgtttttct gttccagcag atctctgact tcagatgaag aattagaagc ttgattattt
     7141 ccttgtgtgc tccctgctgc tcgctgcggc cgtttcttca cctcctcatc cagtttctgc
     7201 tgtagctcct ccactttttg gctgagctca ctctgcccag tcaggccctt agcagatcca
     7261 gaagtcatca ccagaggctg catctgttcc agcaccaaat tgaccttcct cctcacagag
     7321 gtttcggttt ctgagcttcc ctccctcagg atgctataga tagtggcctt cacatggtcc
     7381 acactgcccg gtggggccgt ctcctgctgg tctcggagaa ggtctggagt tgatttgaac
     7441 tgtagcatgc cagggtccca ggctctcccc cgtggctcct caaaactctg aagaacccag
     7501 tcctgggtct gacgggaaca gctaaatcca ccaagagggc cctgattctg ggatgactgt
     7561 ttggagttgc ccatgtggct gtcacgcttt gtgctaggag tccaatgggt agggaactga
     7621 tgttctcgtt catcaagggt atcccgtggg agccggttgt ccaggctttg gctccgcttg
     7681 cgttgttcag ggggcagtgt ccgcatgcgc cggccagtgc ggccccgggc ctgcccaccc
     7741 tggcgactat caaacttgtt gatgagtgag tccactgaag acaggggagc agtgtcaata
     7801 gtgctccctg gggaagctac ttcaggggcc agctgcagca ggctagtgct cctggtgctc
     7861 ggactcacag gggcagggcc tttcagtgat gcctgggact gggatcggag tagccttcca
     7921 ttccaatggg ccccaagctc ctcatcagac atgctgccct gtgagcgggc aggaaattcc
     7981 ttggcatgag agtaggtgct ttcagggagt tcagagtcag agcttagagc tcctggaggc
     8041 cctctgttgt tggtcccctt gatctggacc ccaaaggagt cactaccttg ctccccactg
     8101 ttgagcacca caaagggctg cccagcaatg ccctgcacac gcacagcgac tccataggta
     8161 ttggctctag catccttagc aggacgtcgt ccaccacgac gcagggtgtc catctctgca
     8221 ttacccactg gctctgtgat gaagcgaatc tggactccat ggtctacagg gccccggggc
     8281 tcagccatgc ggccgctctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
     8341 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
     8401 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
     8461 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
     8521 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
     8581 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     8641 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     8701 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     8761 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
     8821 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
     8881 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
     8941 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     9001 cccttgctca ccatggtggc ggatccccgg gtaccgagct cgaattcggg gccgcggagg
     9061 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
     9121 atctgacggt tcactaaacg agctctgctt atataggcct cccaccgtac acgcctacct
     9181 cgacccgggt accgagctcg actttcactt ttctctatca ctgataggga gtggt
//
