LOCUS       sequence-469650-        9652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of Chimera of B-region cCGN in cCGNL1 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Paschoud S, Guillemot L, Citi S
  TITLE     Distinct domains of paracingulin are involved in its targeting to 
            the actin cytoskeleton and regulation of apical junction assembly.
  JOURNAL   J Biol Chem. 2012 Apr 13;287(16):13159-69. doi: 
            10.1074/jbc.M111.315622. Epub 2012 Feb 7.
  PUBMED    22315225
REFERENCE   2  (bases 1 to 9652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.315622"; journalName: "J Biol Chem"; date: 
            "2012-04-13- 13"; volume: "287"; issue: "16"; pages: "13159-69"
COMMENT     Sequence Label: YFP-cCGNL1-cCGN_D-Myc
FEATURES             Location/Qualifiers
     source          1..9652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1793..1811
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    1831..1912
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1856..1875
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2029..3054)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(2029..3054)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(3063..3392)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3077..3212
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    join(3406..3424,3425..3447,3448..3466,3467..3489,3490..3508,
                     3509..3531,3532..3550,3551..3573,3574..3592,3593..3615,
                     3616..3634,3635..3657,3658..3676)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3709..3747
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3744..3768
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3868..3877
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3874..3876,3877..3879,3880..4590)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3919..3940)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4180..4199)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4527..4548
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(4903..4914)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             8191..8220
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          8260..8832
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(8964..8983)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9029..9084
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9083..9102)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9482..9499)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1801 tatcacgagg ccctttcgtc ttcactcgag gcagtgaaaa aaatgcttta tttgtgaaat
     1861 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1921 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     1981 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagaactct attcctttgc
     2041 cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
     2101 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
     2161 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
     2221 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
     2281 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
     2341 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
     2401 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
     2461 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
     2521 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
     2581 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
     2641 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
     2701 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
     2761 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc
     2821 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
     2881 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
     2941 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa
     3001 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatggaagc
     3061 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     3121 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     3181 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     3241 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     3301 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     3361 cctggggact ttccacaccc taactgacac actcgagttt accactccct atcagtgata
     3421 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     3481 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     3541 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     3601 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     3661 ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagg taggcgtgta
     3721 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     3781 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc
     3841 gaattcgagc tcggtacccg gggatccgcc accatggtga gcaagggcga ggagctgttc
     3901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     3961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     4021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     4081 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     4141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     4201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     4261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     4321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     4381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     4441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
     4501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     4561 atcactctcg gcatggacga gctgtacaag agcggccgca tggagctgta tttcggcgaa
     4621 taccaacacg tacagcaaga atatggcgtc catctgagac tcgcaagcga tgatagcaaa
     4681 aagccaagga attcccaaca ctctaaggca ggctcctatg gtgtcagtat tcgggtgcag
     4741 ggaatcgatg gtcaccccta catagtcctg aataacacag agcggtgtct ggcaggcaca
     4801 tcttttcctg aaaatgggcc accatttcca tctccaatga gaagtaacct gcctctgcat
     4861 tccagcaacg ggtccgtgct ggaggagagc agcgcagagg aattgcagct cccagaaaat
     4921 ccgtatgccc agcccagccc agtaagaaac ctgaaacagt cctctctctt cgaaggcaag
     4981 aacggggttc tagaacgcaa agaggggcca atgaagccat cccacgtgtt gaactttcag
     5041 aggcatccag agcttctgca gccctatgac cctgaaaaga atgaatcgaa cttgcaaaac
     5101 caccagcctc ctgagcataa ttggctaaac acattgacag aagaaggtac caacaataag
     5161 aaggcatgga cctgcttccc caagcctggt agttcccagc ctaccagccc tcctttggaa
     5221 gatgcgggca aatccggcgt gacggccatc cgtttgtgca gctctgtggt catcgatgac
     5281 cccaagaagc agacctcagt gtgcgtaaat gttgagagct gcaccgggga ggcgctggta
     5341 ggggaggccc ctaagcttgt agcttcccca gggagcacta ttgacactgc tcccctgtct
     5401 tcagtggact cactcatcaa caagtttgat agtcgccagg gtgggcaggc ccggggccgc
     5461 actggccggc gcatgcggac actgccccct gaacaacgca agcggagcca aagcctggac
     5521 aaccggctcc cacgggatac ccttgatgaa cgagaagatg ggccgtcgca ggtgtgcccg
     5581 cagcggtcac ctgcccagga gcaccgcggg agacagagcg tgggccgcgc cttcgccaag
     5641 ctgcaggggg cccacctccg gcctgcccag cagagcaaag atgggaaagc tccagcaaac
     5701 agagggggtc gggagagtac aaacacgcgt gtgtcctccc ccccagccca gaataaaaag
     5761 gaggacgaaa tcaagacagc cacggcgacg ctgatgttac agaatcgggc cctagcgact
     5821 tcgcccgact ctggtgccaa gaagatttct gtgaagacat ttacttccgc ctccaacact
     5881 caggccacac cagatctgtt gaaaggccaa caagagctca ctcaacaaac caatgaggag
     5941 acggccaagc agatacttta caattacctc aaagaaggaa gcaccgataa tgatgatgcg
     6001 actaaacgga aagtcaacct ggtctttgag aaaatccaga ctttaaagtc tcgagcagca
     6061 gggagcacac aaggaaataa tcaagcttct aattcttcat ctgaagtcag agatctgctg
     6121 gaacagaaaa acaagctgac cacagaagtg gctgaacttc agagacagct tcaactagaa
     6181 gtcaagaatc agcagaacat caaagaagag agagagagaa tgaaagcaaa tttggaggag
     6241 ctccagggcc agcatgatag taaagtggaa gagaactcca tgctacagca gcgcctggaa
     6301 gagagtgaag gggagctccg caagaacctg gaggaattgt tccaggtgaa gatggaacgg
     6361 gaacagcacc agactgagat cagggatctc caggaccagc tctcagaaat gcacgatgaa
     6421 ttggacagtg ctaaacgatc tgaggacagg gaaaagggag ccctgattga ggaactctta
     6481 caggcaaaac aggatcttca ggatctgctc atcgccaaag aggagcagga ggacctcttg
     6541 aggaagcggg agcgtgaact gaccgccctg aagggagccc tgaaggaaga ggtgtccagc
     6601 cacgacctgg agatggataa gctgaaggag cagtatgacg ccgagctgca ggccctgagg
     6661 gagagtgtag aagaagcaac caagaacgtc gaggtcctgg ccagtcggag caacacttca
     6721 gagcagagcc agctgggggc tgaaatgcac gtgaaggctc tgcaggagga gaatgagaag
     6781 ctgcgggaaa gcatcgaaga gctggagcag agcgtcgctc ggctgcagag gcagattgct
     6841 gacatgaagg acgatgaggt caaggcaaag gaaaggctca caaaatgcga gagagaaaac
     6901 cggcaattag aggaggccct tgtgaatgca agaaaggaag aaagagaagc cacatcagtc
     6961 agaaggaccc tggagggtga gctggaagac gtgcagagaa atctgagtcg ggccacccag
     7021 gagcagaagc agttgtctga gaagcttaaa gatgagactg agcagaaaga gcagttaagg
     7081 aggctgaaga acgagatgga gaacgagcga tggcatctgg acaagaccat tgaaaaattg
     7141 cagaaagaga tggctgacat tgttgaggtg tcccgtgtat caacactgga gctccagaac
     7201 cagctagatg agtacaagga gaaaaatcgc agggaacttg cagaaatgca aaggcagttg
     7261 aaggagaaaa cgctagaggc tgaaaagtca cgtctgactg ccatgaaact gcaggatgag
     7321 atgcgtctga tggaggagga gttacgggac taccagagag ctcaggatga ggcgctcaca
     7381 aaaaggcagc ttctggagca gacactcaag gacctggaat acgagctgga ggccaaaagt
     7441 cacctcaaag atgaccgaag cagactcgtc aagcagatgg aggacaaggt gtctcagctg
     7501 gagatggaac tggaagaaga aagaaataac tcggatttgc tctcggagag gatcactcgg
     7561 agcagggaac agatggagca gatgaggagc gagctcctgc aggaaagagc tctcaagcag
     7621 gacttggagt gtgacaagat ttccttggag aggcagaaca aagatttaaa gagccggatt
     7681 atccatctgg aaggctccta caggtccagc aaagaggggc tggtggtgca gatggaggcc
     7741 aggatcgcgg agctggagga ccgtctggaa aacgaggaga gggaccgggc cagcctccag
     7801 ctcagcaacc gacggctgga gcggaaggtg aaggagctag tgatgcaagt ggacgatgag
     7861 cacctgtctc tgactgatca gaaggaccag cttagcttgc gcttgaaagc aatgaaacga
     7921 caagtagagg aggccgagga ggagatcgac agactagaaa gttctaaaaa gaagctgcag
     7981 agggagctgg aggagcagat ggatgtgaat gagcagctgc agggacaact caattccatg
     8041 aagaaggacc taagactcaa gaagctgccg agcaaagtgc tggacgacac ggatgatgat
     8101 gacctcagta cggacggggg gagcctccac gaggcgccgc tgagctacac cttccccaag
     8161 gacagcgctg ccaccagcca gatcgtcgat gaacaaaaac tcatctcaga agaggatctg
     8221 aattaggtcg acgatatctc tagagctgag aacttcaggg tgagtttggg gacccttgat
     8281 tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg
     8341 gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt
     8401 ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct
     8461 gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt
     8521 tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata
     8581 ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga
     8641 atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac
     8701 accctggtca tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg
     8761 aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc
     8821 tctttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa
     8881 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
     8941 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
     9001 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     9061 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     9121 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     9181 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     9241 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     9301 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     9361 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgactgc
     9421 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9481 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9541 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9601 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tt
//
