LOCUS       sequence-469653-        9448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of PLEKHA7 (human) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Paschoud S, Jond L, Guerrera D, Citi S
  TITLE     PLEKHA7 modulates epithelial tight junction barrier function.
  JOURNAL   Tissue Barriers. 2014 Jan 1;2(1):e28755. doi: 10.4161/tisb.28755. 
            Epub 2014 Apr 2.
  PUBMED    24843844
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Tissue 
            Barriers."; date: "2014-01-1"; volume: "2(1):e28755. doi"; pages: " 
            10.4161/tisb.28755. Epub 2014 Apr 2"
COMMENT     Sequence Label: GFP-hPLEKHA7-Myc
FEATURES             Location/Qualifiers
     source          1..9448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      737..746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4114..4143
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          4183..4755
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(4887..4906)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4952..5007
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5006..5025)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5405..5422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5576..6164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5656..5675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6335..7126,7127..7195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6958..6977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7196..7300)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7368..7386
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    7406..7487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7431..7450
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7604..8629)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7604..8629)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8638..8967)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8652..8787
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    join(8981..8999,9000..9022,9023..9041,9042..9064,9065..9083,
                     9084..9106,9107..9125,9126..9148,9149..9167,9168..9190,
                     9191..9209,9210..9232,9233..9251)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9284..9322
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9319..9343
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      join(9443..9448,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagc
      721 ggccgctcta gaccaaccat ggcggcggcg acggtcgggc gggacacttt acctgagcat
      781 tggtcctacg gggtgtgccg ggatggccgc gtcttcttca tcaatgacca gctccgctgc
      841 acgacctggc tgcatccgcg caccggggag cccgtcaact cgggccacat gatccgctca
      901 gacctgcccc gcggctggga ggagggcttc acggaggagg gcgccagcta cttcatcgac
      961 cataaccagc agaccacagc attcaggcat cctgtgacgg gacagttttc tccagaaaat
     1021 agtgaattca ttcttcaaga agagccgaat ccacatatgt cgaagcaaga cagaaaccaa
     1081 agaccgtcca gcatggtcag tgaaacatcc acggctggga ccgcctccac cctggaggcc
     1141 aagcctggac ccaagatcat aaagtccagc agtaaagtcc acagctttgg gaagagagac
     1201 caggccattc ggaggaaccc caatgttccc gtggtggtga ggggctggct gcacaagcag
     1261 gacagttctg ggatgaggct gtggaaaagg aggtggtttg tgcttgctga ttactgctta
     1321 ttttactata aagacagccg agaagaagcg gtcctcggga gcatcccctt gcccagctac
     1381 gtgatctctc ctgtggcccc tgaggatcgc ataagccgca aatattcctt taaggctgtg
     1441 cacacgggga tgcgagcgct catctataac agctccacag cgggctctca ggccgagcag
     1501 tcaggcatga ggacctacta cttcagtgcc gacacccagg aggacatgaa cgcttgggtc
     1561 agggccatga accaggctgc acaggtgctg tctcgatcgt cactgaagag ggatatggag
     1621 aaggtggagc ggcaggctgt cccccaggcc aaccacacag agtcctgtca cgaatgtggc
     1681 cgggtgggac ccggacatac gagagattgt cctcatcgtg gccatgatga cattgtcaac
     1741 ttcgagaggc aggagcagga gggagagcag taccgttccc agagggaccc actggagggc
     1801 aagcgggacc ggagcaaggc caggtctccg tactcgccag ccgaggagga tgccttgttt
     1861 atggatttac ccactggccc aagaggccag caggcacagc cccaacgggc agagaagaat
     1921 ggaatgctgc ctgcctcata tggcccagga gaacagaatg ggactggtgg gtaccagcgg
     1981 gcctttcctc ccaggaccaa ccctgaaaaa cacagccaaa ggaagagcaa tctggcccag
     2041 gtggagcact gggcaagggc ccagaaaggg gatagcagga gtcttccctt ggaccagacg
     2101 cttcctcgcc agggtcctgg ccaatccctg tccttcccag aaaactacca gactcttccc
     2161 aagagcaccc gacacccctc ggggggctcc tcgccacctc cccgaaacct gccaagtgac
     2221 tacaagtatg cgcaggaccg agccagccac ctgaagatgt cgagtgaaga gcgccgggcg
     2281 caccgggatg gcaccgtgtg gcagctctac gagtggcagc agcgccagca gttccggcac
     2341 ggcagcccca cagcgcccat ctgccttggc tccccagagt tcaccgacca gggccggagc
     2401 aggagcatgc tagaggtgcc ccgctccatc tctgtgcctc catctccctc ggacatccct
     2461 cccccaggac ccccaagggt cttcccaccc cggcggccac acacaccagc agagcgagtc
     2521 acagtgaagc caccggacca gaggaggagt gtggacatct cgctggggga ttctccaagg
     2581 agggcacggg gccacgctgt caagaactca tctcatgtgg accgacgttc catgccctcc
     2641 atgggttaca tgacccacac cgtcagcgct cccagtttac atggaaaatc ggctgatgat
     2701 acctacctcc agctgaagaa agacctggag tacctggatc taaagatgac aggccgggac
     2761 cttctcaagg atcgaagtct gaagcctgtg aagatcgctg agagcgacac tgacgtcaaa
     2821 ctgagcatct tctgtgaaca agacagggtc ctccaggact tggaagacaa gatacgagcc
     2881 cttaaagaga acaaagacca gctagaatct gtgctggagg tgttgcacag acagatggag
     2941 cagtaccgag accagcccca gcacttggag aagattgcct accagcagaa gttgctgcag
     3001 gaggaccttg tccatatccg agctgagctc tccagagagt ccactgagat ggaaaatgct
     3061 tggaacgaat acctgaagtt ggagaatgat gtggaacagc tgaagcagac cctgcaggag
     3121 caacacagaa gagccttttt tttccaggag aaatcgcaga tacagaaaga tctatggaga
     3181 attgaagatg tcactgcagg cctgagtgca aataaagaga acttcagaat tctagtggag
     3241 tcagtaaaaa atccggagag aaaaacggtg cctttgtttc ctcacccgcc tgtgccttca
     3301 ctctcaactt ctgagagcaa gccgccccca cagcccagtc ctcccaccag ccctgtgcgg
     3361 acccctctgg aggttcgact cttcccccag ctgcaaacct acgtgccgta ccgacctcac
     3421 ccaccccagc tgaggaaagt gacatccccc cttcagtcac caactaaggc gaagcccaaa
     3481 gttgaagatg aagcacctcc caggccccca ctccccgaac tctacagccc agaggaccag
     3541 cccccggctg tgccgcctct gccaagagag gccaccatca tccggcacac atctgtgcgg
     3601 ggcctcaagc ggcagtcaga cgagaggaag cgagaccggg agctggggca gtgtgtgaat
     3661 ggggattcca gggtggagcc gcggtcgtat gtcagtgagc ctgagctggc gaccctcagc
     3721 ggggacatgg cccagccctc cctaggactt gtgggccctg agagcaggta ccagacgctg
     3781 ccaggcagag ggctctcagg gtccacgtca aggctccagc agtcgtccac cattgctccc
     3841 tacgtcacac tccggagggg tctcaatgcc gaaagcagca aggcgacctt ccctagacct
     3901 aagagtgcct tggagcgcct gtactcaggg gatcaccagc gaggcaagat gagtgcagag
     3961 gagcagctgg agcgcatgaa gcgacaccag aaggccctgg tccgagagcg caagaggaca
     4021 ctgggccaag gggagaggac gggcctgccc tcatctcgct acctcagccg gccgctccct
     4081 ggtgatcttg gctcagtatg taagcttatc gatgaacaaa aactcatctc agaagaggat
     4141 ctgaattagg tcgacgatat ctctagagct gagaacttca gggtgagttt ggggaccctt
     4201 gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc
     4261 agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt
     4321 tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt
     4381 tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt
     4441 gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt
     4501 atattatatt gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt
     4561 agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac
     4621 tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag
     4681 atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc
     4741 ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca tcattttggc
     4801 aaagaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     4861 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     4921 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     4981 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     5041 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     5101 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     5161 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     5221 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     5281 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     5341 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5401 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5461 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5521 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5581 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5641 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5701 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5761 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5821 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5881 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5941 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6001 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6061 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6121 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6181 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6241 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6301 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6361 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6421 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6481 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6541 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6601 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6661 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6721 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6781 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6841 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6901 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     6961 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7021 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7081 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7141 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7201 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7261 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7321 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     7381 cgaggccctt tcgtcttcac tcgaggcagt gaaaaaaatg ctttatttgt gaaatttgtg
     7441 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     7501 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     7561 acctctacaa atgtggtatg gctgattatg atcctctaga actctattcc tttgccctcg
     7621 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     7681 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     7741 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     7801 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     7861 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     7921 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     7981 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     8041 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     8101 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     8161 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     8221 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     8281 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     8341 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
     8401 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     8461 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     8521 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     8581 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcatg gaagcttttt
     8641 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     8701 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     8761 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     8821 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     8881 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     8941 ggactttcca caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa
     9001 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     9061 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     9121 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     9181 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     9241 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
     9301 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
     9361 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
     9421 cgagctcggt acccggggat ccgccacc
//
