LOCUS       sequence-46968-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan 
            EK
  TITLE     Divergent GW182 functional domains in the regulation of 
            translational silencing.
  JOURNAL   Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2.
  PUBMED    21131274
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-04"; volume: "39(7)"; pages: "2534-47. Epub
            2010 Dec 2"
COMMENT     Sequence Label: NHA-TNGW1
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          174..306
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     289..308
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        351..369
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      373..382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             379..444
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             481..507
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             487..513
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    565..589
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggctttag cgactgttag tgaaccgtca gatcactaga agctttattg cggtagttta
       61 tcacagttaa attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg
      121 cagtgactct cttaaggtag ccttgcagaa gttggtcgtg aggcactggg caggtaagta
      181 tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa
      241 gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt gcctttctct
      301 ccacaggtgt ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact
      361 cactataggc tagccaccat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa
      421 caagctcaat ggaaagctgc aaacccaccg ctcgaggctg cccaagctta caccatggcc
      481 tacccatatg atgttccaga ttacgcttca ctcgacgcgg atcccgtcga attcacgcgt
      541 ggtacctcta gagtcgaccc atcaacaagt ttgtacaaaa aagcaggctc tttaaaggaa
      601 ccaattcagt cgactggatc tatgagagaa ttggaagcta aagctaccaa agacgtagaa
      661 agaaatctta gcagggattt agtgcaagaa gaagaacagt tgatggaaga aaagaaaaag
      721 aaaaaagacg acaagaaaaa gaaggaagct gctcaaaaga aggccactga acaaaaaatc
      781 aaagtgccag aacagataaa gcccagtgta agccagcctc agcctgccaa ctctaaa
//
