LOCUS       sequence-469699-        9235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Helper plasmid for regulated phage transfer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Kusumawardhani, H., Zoppi, F., Avendano, R. et al.
  TITLE     Engineering intercellular communication using M13 phagemid and 
            CRISPR-based gene regulation for multicellular computing in 
            Escherichia coli
  JOURNAL   Nat Commun 16, 3569 (2025)
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            16, 3569 (2025)"
COMMENT     Sequence Label: HP17deltaP8
FEATURES             Location/Qualifiers
     source          1..9235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     686..705
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     770..789
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(1136..2086)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFGIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2134..2355
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2847..2941)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(3097..3126)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             3337..4569
                     /codon_start=1
                     /gene="II"
                     /product="pII"
                     /label=M13 gene II
                     /note="replication"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSAR
                     TVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
                     FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
                     RNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKMTSYQ
                     KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
                     DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
                     VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
                     DWYNEPVLKIA"
     CDS             4581..4844
                     /codon_start=1
                     /gene="V"
                     /product="pV"
                     /label=M13 gene V
                     /note="replication"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
                     KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             4846..4947
                     /codon_start=1
                     /gene="VII"
                     /product="pVII"
                     /label=M13 gene VII
                     /note="minor coat protein"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             4944..5042
                     /codon_start=1
                     /gene="IX"
                     /product="pIX"
                     /label=M13 gene IX
                     /note="minor coat protein"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     terminator      5051..5099
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             5104..6378
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     CDS             6381..6719
                     /codon_start=1
                     /gene="VI"
                     /product="pVI"
                     /label=M13 gene VI
                     /note="minor coat protein"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     CDS             6721..7767
                     /codon_start=1
                     /gene="I"
                     /product="pI"
                     /label=M13 gene I
                     /note="phage assembly"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
                     VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
                     QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
                     ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
                     TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
                     SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
                     SNEIVKCN"
     CDS             7745..9025
                     /codon_start=1
                     /gene="IV"
                     /product="pIV"
                     /label=M13 gene IV
                     /note="phage assembly"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     terminator      9068..9099
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(join(9198..9235,1..778))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
       61 aaaactcacc gaggcagttc caaagaatgg caaggtcctg gtaacggtct gcgattccga
      121 cccgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
      181 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caagagcttg tgcatttctt
      241 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
      301 aaccgttatt catgcgtgat tgcgcctgag caagacgaaa tacacgatcg ctgttaaaag
      361 gacaattaca aacaggaatc gaatgtaacc ggcgcaggaa cacggccagc gcatcaacaa
      421 tattttcacc tgaatcagga tattcttcta atacctggaa ggctgttttc ccaggaatcg
      481 cggtggtgag taaccacgca tcatcaggag tacggataaa atgcttgatg gtcgggagag
      541 gcataaactc cgtcagccag ttgagacgga ccatctcatc tgtaacatca ttggcaacgc
      601 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aagcgataga
      661 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcgt
      721 ccatgttgga gtttaagcgc ggacgggagc aagacgtttc ccgttgaata tggctcataa
      781 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
      841 tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc
      901 gaacttttgc tgagttgaag gatcagctct agtagttaca ttgtcgatct gttcatggtg
      961 aacagctttg aatgcaccaa aaactcgtaa aagctctgat gtatctatct tttttacacc
     1021 gttttcatct gtgcatatgg acagttttcc ctttgatatg taacggtgaa cagttgttct
     1081 acttttgttt gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga
     1141 tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg
     1201 agaacgaacc attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac
     1261 tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta
     1321 ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt
     1381 tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat
     1441 cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt
     1501 tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa
     1561 gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct
     1621 tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag
     1681 acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata
     1741 tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact
     1801 ggaaaatccc aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct
     1861 ctctggttgc tttagctaat acaccataag cattttccct actgatgttc atcatctgag
     1921 cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg
     1981 ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc
     2041 gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt
     2101 ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa ttacatgtcc
     2161 ttttcctttg agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa
     2221 attctgctag accctctgta aattccgcta gacctttgtg tgtttttttg tttatattca
     2281 agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag
     2341 ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac
     2401 gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc
     2461 aagctcgggc aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct
     2521 gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat
     2581 ggcactacag gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc
     2641 ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt
     2701 ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt
     2761 gacaggtcat tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta
     2821 cccgtcttac tgtccctagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     2881 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     2941 ccaagcgagc tcgtaaactt ggtctgacag ctctagctcc ggcaaaaaaa cgggcaaggt
     3001 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgttt gccacctgac
     3061 gtctaagaaa aggaatattc agcaatttgc ccgtgccgaa gaaaggccca cccgtgaagg
     3121 tgagccagtg agttgattgc tacgtaatta gttagttagc ccttagtgac tccagacgcg
     3181 aattattttt gatggcgttc ctattggtta aaaaatgagc tgatttaaca aaaatttaat
     3241 gcgaatttta acaaaatatt aacgtttaca atttaaatat ttgcttatac aatcttcctg
     3301 tttttggggc ttttctgatt atcaaccggg gtacatatga ttgacatgct agttttacga
     3361 ttaccgttca tcgattctct tgtttgctcc agactctcag gcaatgacct gatagccttt
     3421 gtagacctct caaaaatagc taccctctcc ggcatgaatt tatcagctag aacggttgaa
     3481 tatcatattg atggtgattt gactgtctcc ggcctttctc acccttttga atctttacct
     3541 acacattact caggcattgc atttaaaata tatgagggtt ctaaaaattt ttatccttgc
     3601 gttgaaataa aggcttctcc cgcaaaagta ttacagggtc ataatgtttt tggtacaacc
     3661 gatttagctt tatgctctga ggctttattg cttaattttg ctaattcttt gccttgcctg
     3721 tatgatttat tggatgttaa cgctactact attagtagaa ttgatgccac cttttcagct
     3781 cgcgccccaa atgaaaatat agctaaacag gttattgacc atttgcgaaa tgtatctaat
     3841 ggtcaaacta aatctactcg ttcgcagaat tgggaatcaa ctgttacatg gaatgaaact
     3901 tccagacacc gtactttagt tgcatattta aaacatgttg agctacagca ccagattcag
     3961 caattaagct ctaagccatc cgcaaaaatg acctcttatc aaaaggagca attaaaggta
     4021 ctctctaatc ctgacctgtt ggagtttgct tccggtctgg ttcgctttga agctcgaatt
     4081 aaaacgcgat atttgaagtc tttcgggctt cctcttaatc tttttgatgc aatccgcttt
     4141 gcttctgact ataatagtca gggtaaagac ctgatttttg atttatggtc attctcgttt
     4201 tctgaactgt ttaaagcatt tgagggggat tcaatgaata tttatgacga ttccgcagta
     4261 ttggacgcta tccagtctaa acattttact attaccccct ctggcaaaac ttcttttgca
     4321 aaagcctctc gctattttgg tttttatcgt cgtctggtaa acgagggtta tgatagtgtt
     4381 gctcttacta tgcctcgtaa ttccttttgg cgttatgtat ctgcattagt tgaatgtggt
     4441 attcctaaat ctcaactgat gaatctttct acctgtaata atgttgttcc gttagttcgt
     4501 tttattaacg tagatttttc ttcccaacgt cctgactggt ataatgagcc agttcttaaa
     4561 atcgcataag gtaattcaca atgattaaag ttgaaattaa accatctcaa gcccaattta
     4621 ctactcgttc tggtgtttct cgtcagggca agccttattc actgaatgag cagctttgtt
     4681 acgttgattt gggtaatgaa tatccggttc ttgtcaagat tactcttgat gaaggtcagc
     4741 cagcctatgc gcctggtctg tacaccgttc atctgtcctc tttcaaagtt ggtcagttcg
     4801 gttcccttat gattgaccgt ctgcgcctcg ttccggctaa gtaacatgga gcaggtcgcg
     4861 gatttcgaca caatttatca ggcgatgata caaatctccg ttgtactttg tttcgcgctt
     4921 ggtataatcg ctgggggtca aagatgagtg ttttagtgta ttctttcgcc tctttcgttt
     4981 taggttggtg ccttcgtagt ggcattacgt attttacccg tttaatggaa acttcctcat
     5041 gagctgataa accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc
     5101 aacgtgaaaa aattattatt cgcaattcct ttagttgttc ctttctattc tcactccgct
     5161 gaaactgttg aaagttgttt agcaaaaccc catacagaaa attcatttac taacgtctgg
     5221 aaagacgaca aaactttaga tcgttacgct aactatgagg gctgtctgtg gaatgctaca
     5281 ggcgttgtag tttgtactgg tgacgaaact cagtgttacg gtacatgggt tcctattggg
     5341 cttgctatcc ctgaaaatga gggtggtggc tctgagggtg gcggttctga gggtggcggt
     5401 tctgagggtg gcggtactaa acctcctgag tacggtgata cacctattcc gggctatact
     5461 tatatcaacc ctctcgacgg cacttatccg cctggtactg agcaaaaccc cgctaatcct
     5521 aatccttctc ttgaggagtc tcagcctctt aatactttca tgtttcagaa taataggttc
     5581 cgaaataggc agggggcatt aactgtttat acgggcactg ttactcaagg cactgacccc
     5641 gttaaaactt attaccagta cactcctgta tcatcaaaag ccatgtatga cgcttactgg
     5701 aacggtaaat tcagagactg cgctttccat tctggcttta atgaggatcc attcgtttgt
     5761 gaatatcaag gccaatcgtc tgacctgcct caacctcctg tcaatgctgg cggcggctct
     5821 ggtggtggtt ctggtggcgg ctctgagggt ggtggctctg agggtggcgg ttctgagggt
     5881 ggcggctctg agggaggcgg ttccggtggt ggctctggtt ccggtgattt tgattatgaa
     5941 aagatggcaa acgctaataa gggggctatg accgaaaatg ccgatgaaaa cgcgctacag
     6001 tctgacgcta aaggcaaact tgattctgtc gctactgatt acggtgctgc tatcgatggt
     6061 ttcattggtg acgtttccgg ccttgctaat ggtaatggtg ctactggtga ttttgctggc
     6121 tctaattccc aaatggctca agtcggtgac ggtgataatt cacctttaat gaataatttc
     6181 cgtcaatatt taccttccct ccctcaatcg gttgaatgtc gcccttttgt ctttggcgct
     6241 ggtaaaccat atgaattttc tattgattgt gacaaaataa acttattccg tggtgtcttt
     6301 gcgtttcttt tatatgttgc cacctttatg tatgtatttt ctacgtttgc taacatactg
     6361 cgtaataagg agtcttaatc atgccagttc ttttgggtat tccgttatta ttgcgtttcc
     6421 tcggtttcct tctggtaact ttgttcggct atctgcttac ttttcttaaa aagggcttcg
     6481 gtaagatagc tattgctatt tcattgtttc ttgctcttat tattgggctt aactcaattc
     6541 ttgtgggtta tctctctgat attagcgctc aattaccctc tgactttgtt cagggtgttc
     6601 agttaattct cccgtctaat gcgcttccct gtttttatgt tattctctct gtaaaggctg
     6661 ctattttcat ttttgacgtt aaacaaaaaa tcgtttctta tttggattgg gataaataat
     6721 atggctgttt attttgtaac tggcaaatta ggctctggaa agacgctcgt tagcgttggt
     6781 aagattcagg ataaaattgt agctgggtgc aaaatagcaa ctaatcttga tttaaggctt
     6841 caaaacctcc cgcaagtcgg gaggttcgct aaaacgcctc gcgttcttag aataccggat
     6901 aagccttcta tatctgattt gcttgctatt gggcgcggta atgattccta cgatgaaaat
     6961 aaaaacggct tgcttgttct cgatgagtgc ggtacttggt ttaatacccg ttcttggaat
     7021 gataaggaaa gacagccgat tattgattgg tttctacatg ctcgtaaatt aggatgggat
     7081 attatttttc ttgttcagga cttatctatt gttgataaac aggcgcgttc tgcattagct
     7141 gaacatgttg tttattgtcg tcgtctggac agaattactt taccttttgt cggtacttta
     7201 tattctctta ttactggctc gaaaatgcct ctgcctaaat tacatgttgg cgttgttaaa
     7261 tatggcgatt ctcaattaag ccctactgtt gagcgttggc tttatactgg taagaatttg
     7321 tataacgcat atgatactaa acaggctttt tctagtaatt atgattccgg tgtttattct
     7381 tatttaacgc cttatttatc acacggtcgg tatttcaaac cattaaattt aggtcagaag
     7441 atgaaattaa ctaaaatata tttgaaaaag ttttctcgcg ttctttgtct tgcgattgga
     7501 tttgcatcag catttacata tagttatata acccaaccta agccggaggt taaaaaggta
     7561 gtctctcaga cctatgattt tgataaattc actattgact cttctcagcg tcttaatcta
     7621 agctatcgct atgttttcaa ggattctaag ggaaaattaa ttaatagcga cgatttacag
     7681 aagcaaggtt attcactcac atatattgat ttatgtactg tttccattaa aaaaggtaat
     7741 tcaaatgaaa ttgttaaatg taattaattt tgttttcttg atgtttgttt catcatcttc
     7801 ttttgctcag gtaattgaaa tgaataattc gcctctgcgc gattttgtaa cttggtattc
     7861 aaagcaatca ggcgaatccg ttattgtttc tcccgatgta aaaggtactg ttactgtata
     7921 ttcatctgac gttaaacctg aaaatctacg caatttcttt atttctgttt tacgtgcaaa
     7981 taattttgat atggtaggtt ctaacccttc cattattcag aagtataatc caaacaatca
     8041 ggattatatt gatgaattgc catcatctga taatcaggaa tatgatgata attccgctcc
     8101 ttctggtggt ttctttgttc cgcaaaatga taatgttact caaactttta aaattaataa
     8161 cgttcgggca aaggatttaa tacgagttgt cgaattgttt gtaaagtcta atacttctaa
     8221 atcctcaaat gtattatcta ttgacggctc taatctatta gttgttagtg ctcctaaaga
     8281 tattttagat aaccttcctc aattcctttc aactgttgat ttgccaactg accagatatt
     8341 gattgagggt ttgatatttg aggttcagca aggtgatgct ttagattttt catttgctgc
     8401 tggctctcag cgtggcactg ttgcaggcgg tgttaatact gaccgcctca cctctgtttt
     8461 atcttctgct ggtggttcgt tcggtatttt taatggcgat gttttagggc tatcagttcg
     8521 cgcattaaag actaatagcc attcaaaaat attgtctgtg ccacgtattc ttacgctttc
     8581 aggtcagaag ggttctatct ctgttggcca gaatgtccct tttattactg gtcgtgtgac
     8641 tggtgaatct gccaatgtaa ataatccatt tcagacgatt gagcgtcaaa atgtaggtat
     8701 ttccatgagc gtttttcctg ttgcaatggc tggcggtaat attgttctgg atattaccag
     8761 caaggccgat agtttgagtt cttctactca ggcaagtgat gttattacta atcaaagaag
     8821 tattgctaca acggttaatt tgcgtgatgg acagactctt ttactcggtg gcctcactga
     8881 ttataaaaac acttctcagg attctggcgt accgttcctg tctaaaatcc ctttaatcgg
     8941 cctcctgttt agctcccgct ctgattctaa cgaggaaagc acgttatacg tgctcgtcaa
     9001 agcaaccata gtacgcgccc tgtagcatgc aggagtcact aagggttagt tagttagatt
     9061 agcagaaagt caaaagcctc cgaccggagg cttttgacta aaacttccct tggggttatc
     9121 attggggctc actcaaaggc ggtaatcaga taaaaaaaat ccttagcttt cgctaaggat
     9181 gatttctgct agtattatta gaaaaactca tcgagcatca aatgaaactg caatt
//
