LOCUS       sequence-469723-        4490 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of mouse Smad7 with stop codon (for native 
            or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4490)
  TITLE     Esposito lab plasmids 2025
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 8061-E37
FEATURES             Location/Qualifiers
     source          1..4490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(1971..2070)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2087..2106)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2088..2106)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2111..2127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2111..2127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2558..3349
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3471..3580
                     /note="incP origin of transfer"
     rep_origin      3840..4428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4329..4348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgttcagg accaaacgat ctgcgctcgt
      721 ccggcgtctc tggaggagcc gtgcgcccgg cggcgaggac gaggaggagg gcgtgggggg
      781 tggcggcgga ggaggcgagc tgcggggaga aggggcgacg gacggccggg cttatggggc
      841 tggtggcggc ggtgcgggca gggctggctg ctgcctgggc aaggcagtcc gaggtgccaa
      901 aggtcaccac catccccatc ccccaacctc gggtgccggg gcggccgggg gcgccgaggc
      961 ggatctgaag gcgctcacgc actcggtgct caagaaactc aaggagcggc agctggagct
     1021 gctgcttcag gccgtggagt cccgcggcgg tacgcgcacc gcgtgcctcc tgctgcccgg
     1081 ccgcctggac tgcaggctgg gcccgggggc gcccgccagc gcgcagcccg cgcagccgcc
     1141 ctcgtcctac tcgctccccc tcctgctgtg caaagtgttc aggtggccgg atctcaggca
     1201 ttcctcggaa gtcaagaggc tgtgttgctg tgaatcttac gggaagatca accccgagct
     1261 ggtgtgctgc aacccccatc accttagtcg actctgtgaa ctagagtctc cccctcctcc
     1321 ttactccaga tacccaatgg attttctcaa accaactgca ggctgtccag atgctgtacc
     1381 ttcctccgcg gaaaccgggg gaacgaatta tctggcccct ggggggcttt cagattccca
     1441 acttcttctg gagcctgggg atcggtcaca ctggtgcgtg gtggcatact gggaggagaa
     1501 gactcgcgtg gggaggctct actgtgtcca agagccctcc ctggatatct tctatgatct
     1561 acctcagggg aatggctttt gcctcggaca gctcaattcg gacaacaaga gtcagctggt
     1621 acagaaagtg cggagcaaga tcggctgtgg catccagctg acgcgggaag tggatggcgt
     1681 gtgggtttac aaccgcagca gttaccccat cttcatcaag tccgccacac tggacaaccc
     1741 ggactccagg acgctgttgg tgcacaaagt gttccctggt ttctccatca aggcttttga
     1801 ctatgagaaa gcctacagcc tgcagcggcc caatgaccac gagttcatgc agcaaccatg
     1861 gacgggtttc accgtgcaga tcagctttgt gaagggctgg ggccagtgct acacccgcca
     1921 gttcatcagc agctgcccgt gctggctgga ggtcatcttc aacagccggt aatcaacttt
     1981 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2041 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2101 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2161 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     2221 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     2281 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     2341 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     2401 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     2461 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     2521 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     2581 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     2641 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     2701 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     2761 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     2821 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     2881 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     2941 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     3001 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     3061 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     3121 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     3181 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     3241 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     3301 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgactct
     3361 agaggatccc cgggtaccga gctcgaattc cgtgataggt gggctgccct tcctggttgg
     3421 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
     3481 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
     3541 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
     3601 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
     3661 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaacggaat
     3721 tcgagctcgg tacccgggga tcctctagag tcgagccacc catgaccaaa atcccttaac
     3781 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3841 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3901 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3961 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4021 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4081 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4141 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4201 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4261 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4321 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4381 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4441 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
