LOCUS       sequence-469727-        4475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human SMAD3 truncated with stop codon 
            (for native or N-terminal fusions); C-term truncation to delete 
            phosphorylation sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4475)
  TITLE     Esposito lab plasmids 2025
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 9336-E24
FEATURES             Location/Qualifiers
     source          1..4475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(1956..2055)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2072..2091)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2073..2091)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2096..2112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2096..2112)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2543..3334
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3456..3565
                     /note="incP origin of transfer"
     rep_origin      3825..4413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4314..4333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgtcgtcc atcctgcctt tcactccccc
      721 gatcgtgaag cgcctgctgg gctggaagaa gggcgagcag aacgggcagg aggagaaatg
      781 gtgcgagaag gcggtcaaga gcctggtcaa gaaactcaag aagacggggc agctggacga
      841 gctggagaag gccatcacca cgcagaacgt caacaccaag tgcatcacca tccccaggtc
      901 cctggatggc cggttgcagg tgtcccatcg gaaggggctc cctcatgtca tctactgccg
      961 cctgtggcga tggccagacc tgcacagcca ccacgagctg cgggccatgg agctgtgtga
     1021 gttcgccttc aatatgaaga aggacgaggt ctgcgtgaat ccctaccact accagagagt
     1081 agagacacca gttctacctc ctgtgttggt gccacgccac acagagatcc cggccgagtt
     1141 ccccccactg gacgactaca gccattccat ccccgaaaac actaacttcc ccgcaggcat
     1201 cgagccccag agcaatattc cagagacccc accccctggc tacctgagtg aagatggaga
     1261 aaccagtgac caccagatga accacagcat ggacgcaggt tctccaaacc tatccccgaa
     1321 tccgatgtcc ccagcacata ataacttgga cctgcagcca gttacctact gcgagccggc
     1381 cttctggtgc tccatctcct actacgagct gaaccagcgc gtcggggaga cattccacgc
     1441 ctcgcagcca tccatgactg tggatggctt caccgacccc tccaattcgg agcgcttctg
     1501 cctagggctg ctctccaatg tcaacaggaa tgcagcagtg gagctgacac ggagacacat
     1561 cggaagaggc gtgcggctct actacatcgg aggggaggtc ttcgcagagt gcctcagtga
     1621 cagcgctatt tttgtccagt ctcccaactg taaccagcgc tatggctggc acccggccac
     1681 cgtctgcaag atcccaccag gatgcaacct gaagatcttc aacaaccagg agttcgctgc
     1741 cctcctggcc cagtcggtca accagggctt tgaggctgtc taccagttga cccgaatgtg
     1801 caccatccgc atgagcttcg tcaaaggctg gggagcggag tacaggagac agactgtgac
     1861 cagtaccccc tgctggattg agctgcacct gaatgggcct ttgcagtggc ttgacaaggt
     1921 cctcacccag atgggctccc caagcatccg ctgttaatca actttcttgt acaaagttgg
     1981 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2041 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     2101 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2161 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     2221 gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     2281 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     2341 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     2401 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     2461 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     2521 ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact
     2581 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     2641 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     2701 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     2761 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     2821 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     2881 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     2941 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     3001 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     3061 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     3121 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     3181 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     3241 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     3301 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg actctagagg atccccgggt
     3361 accgagctcg aattccgtga taggtgggct gcccttcctg gttggcttgg tttcatcagc
     3421 catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt
     3481 cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg
     3541 tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac
     3601 acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat
     3661 cgctataatg accccgaagc agggttatgc agcggaaaac ggaattcgag ctcggtaccc
     3721 ggggatcctc tagagtcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3781 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3841 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3901 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3961 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4021 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4081 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4141 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4201 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4261 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4321 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4381 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4441 ttcctggcct tttgctggcc ttttgctcac atgtt
//
