LOCUS       sequence-469773-        4691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human ACVR1C with stop codon (for native 
            or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4691)
  TITLE     Esposito lab plasmids 2025
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 8061-E20
FEATURES             Location/Qualifiers
     source          1..4691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2172..2271)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2288..2307)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2289..2307)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2312..2328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2312..2328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2759..3550
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3672..3781
                     /note="incP origin of transfer"
     rep_origin      4041..4629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4530..4549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgacccgg gcgctctgct cagcgctccg
      721 ccaggctctc ctgctgctcg cagcggccgc cgagctctcg ccaggactga agtgtgtatg
      781 tcttttgtgt gattcttcaa actttacctg ccaaacagaa ggagcatgtt gggcatcagt
      841 catgctaacc aatggaaaag agcaggtgat caaatcctgt gtctcccttc cagaactgaa
      901 tgctcaagtc ttctgtcata gttccaacaa tgttaccaaa accgaatgct gcttcacaga
      961 tttttgcaac aacataacac tgcaccttcc aacagcatca ccaaatgccc caaaacttgg
     1021 acccatggag ctggccatca ttattactgt gcctgtttgc ctcctgtcca tagctgcgat
     1081 gctgacagta tgggcatgcc agggtcgaca gtgctcctac aggaagaaaa agagaccaaa
     1141 tgtggaggaa ccactctctg agtgcaatct ggtaaatgct ggaaaaactc tgaaagatct
     1201 gatttatgat gtgaccgcct ctggatctgg ctctggtcta cctctgttgg ttcaaaggac
     1261 aattgcaagg acgattgtgc ttcaggaaat agtaggaaaa ggtagatttg gtgaggtgtg
     1321 gcatggaaga tggtgtgggg aagatgtggc tgtgaaaata ttctcctcca gagatgaaag
     1381 atattggttt cgtgaggcag aaatttacca gacggtcatg ctgcgacatg aaaacatcct
     1441 tggtttcatt gctgctgaca acaaagataa tggaacttgg actcaacttt ggctggtatc
     1501 tgaatatcat gaacagggct ccttatatga ctatttgaat agaaatatag tgaccatggc
     1561 tggaatgatc aagctggcgc tctcaattgc tagtggtctg gcacaccttc atatggagat
     1621 tgttggtaca caaggtaaac ctgctattgc tcatcgagac ataaaatcaa agaatatctt
     1681 agtaaaaaag tgtgaaactt gtgccatagc ggacttaggg ttggctgtga agcatgattc
     1741 aatactgaac actatcgaca tacctcagaa tcctaaagtg ggaaccaaga ggtatatggc
     1801 tcctgaaatg cttgatgata caatgaatgt gaatatcttt gagtccttca aacgagctga
     1861 catctattct gttggtctgg tttactggga aatagcccgg aggtgttcag tcggaggaat
     1921 tgttgaggag taccaattgc cttattatga catggtgcct tcagatccct cgatagagga
     1981 aatgagaaag gttgtttgtg accagaagtt tcgaccaagt atcccaaacc agtggcaaag
     2041 ttgtgaagca ctccgagtca tggggagaat aatgcgtgag tgttggtatg ccaacggagc
     2101 ggcccgccta actgctcttc gtattaagaa gactatatct caactttgtg tcaaagaaga
     2161 ctgcaaagcc taatcaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     2221 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2281 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2341 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct
     2401 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
     2461 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     2521 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     2581 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     2641 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     2701 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
     2761 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     2821 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     2881 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     2941 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     3001 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     3061 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     3121 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     3181 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     3241 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     3301 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     3361 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     3421 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     3481 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     3541 cggcaaataa ccctcgactc tagaggatcc ccgggtaccg agctcgaatt ccgtgatagg
     3601 tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac
     3661 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     3721 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     3781 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     3841 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     3901 ttatgcagcg gaaaacggaa ttcgagctcg gtacccgggg atcctctaga gtcgagccac
     3961 ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     4021 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4081 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4141 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     4201 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4261 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4321 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4381 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4441 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4501 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4561 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4621 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4681 gctcacatgt t
//
