LOCUS       sequence-469779-        4751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human ACVR2A with stop codon (for native 
            or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4751)
  TITLE     Esposito lab plasmids 2025
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 8061-E21
FEATURES             Location/Qualifiers
     source          1..4751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2232..2331)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2348..2367)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2349..2367)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2372..2388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2372..2388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2819..3610
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3732..3841
                     /note="incP origin of transfer"
     rep_origin      4101..4689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4590..4609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgggagct gctgcaaagt tggcgtttgc
      721 cgtctttctt atctcctgtt cttcaggtgc tatacttggt agatcagaaa ctcaggagtg
      781 tcttttcttt aatgctaatt gggaaaaaga cagaaccaat caaactggtg ttgaaccgtg
      841 ttatggtgac aaagataaac ggcggcattg ttttgctacc tggaagaata tttctggttc
      901 cattgaaata gtgaaacaag gttgttggct ggatgatatc aactgctatg acaggactga
      961 ttgtgtagaa aaaaaagaca gccctgaagt atatttttgt tgctgtgagg gcaatatgtg
     1021 taatgaaaag ttttcttatt ttccagagat ggaagtcaca cagcccactt caaatccagt
     1081 tacacctaag ccaccctatt acaacatcct gctctattcc ttggtgccac ttatgttaat
     1141 tgcggggatt gtcatttgtg cattttgggt gtacaggcat cacaagatgg cctaccctcc
     1201 tgtacttgtt ccaactcaag acccaggacc acccccacct tctccattac taggtttgaa
     1261 accactgcag ttattagaag tgaaagcaag gggaagattt ggttgtgtct ggaaagccca
     1321 gttgcttaac gaatatgtgg ctgtcaaaat atttccaata caggacaaac agtcatggca
     1381 aaatgaatac gaagtctaca gtttgcctgg aatgaagcat gagaacatat tacagttcat
     1441 tggtgcagaa aaacgaggca ccagtgttga tgtggatctt tggctgatca cagcatttca
     1501 tgaaaagggt tcactatcag actttcttaa ggctaatgtg gtctcttgga atgaactgtg
     1561 tcatattgca gaaaccatgg ctagaggatt ggcatattta catgaggata tacctggcct
     1621 aaaagatggc cacaaacctg ccatatctca cagggacatc aaaagtaaaa atgtgctgtt
     1681 gaaaaacaac ctgacagctt gcattgctga ctttgggttg gccttaaaat ttgaggctgg
     1741 caagtctgca ggcgataccc atggacaggt tggtacccgg aggtacatgg ctccagaggt
     1801 attagagggt gctataaact tccaaaggga tgcatttttg aggatagata tgtatgccat
     1861 gggattagtc ctatgggaac tggcttctcg ctgtactgct gcagatggac ctgtagatga
     1921 atacatgttg ccatttgagg aggaaattgg ccagcatcca tctcttgaag acatgcagga
     1981 agttgttgtg cataaaaaaa agaggcctgt tttaagagat tattggcaga aacatgctgg
     2041 aatggcaatg ctctgtgaaa ccattgaaga atgttgggat cacgacgcag aagccaggtt
     2101 atcagctgga tgtgtaggtg aaagaattac ccagatgcag agactaacaa atattattac
     2161 cacagaggac attgtaacag tggtcacaat ggtgacaaat gttgactttc ctcccaaaga
     2221 atctagtcta taatcaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     2281 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2341 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2401 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct
     2461 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
     2521 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     2581 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     2641 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     2701 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     2761 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
     2821 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     2881 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     2941 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     3001 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     3061 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     3121 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     3181 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     3241 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     3301 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     3361 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     3421 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     3481 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     3541 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     3601 cggcaaataa ccctcgactc tagaggatcc ccgggtaccg agctcgaatt ccgtgatagg
     3661 tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac
     3721 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     3781 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     3841 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     3901 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     3961 ttatgcagcg gaaaacggaa ttcgagctcg gtacccgggg atcctctaga gtcgagccac
     4021 ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     4081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     4261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4741 gctcacatgt t
//
