LOCUS       sequence-46978-e        1750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Duncan JS, Gyenis L, Lenehan J, Bretner M, Graves LM, Haystead TA, 
            Litchfield DW
  TITLE     An unbiased evaluation of CK2 inhibitors by chemoproteomics: 
            characterization of inhibitor effects on CK2 and identification of 
            novel inhibitor targets.
  JOURNAL   Mol Cell Proteomics. 2008 Jun;7(6):1077-88. Epub 2008 Feb 7.
  PUBMED    18258654
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2008-06"; volume: "7(6)"; pages: "1077-88. Epub
            2008 Feb 7"
COMMENT     Sequence Label: CK2alpha V66A/I174A-HA  (pJD23)
FEATURES             Location/Qualifiers
     source          1..1750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1414..1503
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(1572..1590)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1573..1590)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1610..1627)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1616..1727
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttaact ggcttatcga
       61 aattaatacg actcactata gggagaccca agcttggtac cgagctccac cgcggtggcg
      121 gccgctctag aactactgga tccaggtctg aaacaaggtc ttacccccag ctgcttctga
      181 acacagtgac tgccagatct ccaaacatca agtccagctt tgtccgccaa cctgtctgac
      241 atgtcgggac ccgtgccaag cagggccaga gtttacacag atgttaatac acacagacct
      301 cgagaatact gggattacga gtcacatgtg gtggaatggg gaaatcaaga tgactaccag
      361 ctggttcgaa aattaggccg aggtaaatac agtgaagtat ttgaagccat caacatcaca
      421 aataatgaaa aagttgcagt taaaattctc aagccagtaa aaaagaagaa aattaagcgt
      481 gaaataaaga ttttggagaa tttgagagga ggtcccaaca tcatcacact ggcagacatt
      541 gtaaaagacc ctgtgtcacg aacccccgcc ttggtttttg aacacgtaaa caacacagac
      601 ttcaagcaat tgtaccagac gttaacagac tatgatattc gattttacat gtatgagatt
      661 ctgaaggccc tggattattg tcacagcatg ggaattatgc acagagatgt caagccccat
      721 aatgtcatga ttgatcatga gcacagaaag ctacgactag cagactgggg tttggctgag
      781 ttttatcatc ctggccaaga atataatgtc cgagttgctt cccgatactt caaaggtcct
      841 gagctacttg tagactatca gatgtacgat tatagtttgg atatgtggag tttgggttgt
      901 atgctggcaa gtatgatctt tcggaaggag ccatttttcc atggacatga caattatgat
      961 cagttggtga ggatagccaa ggttctgggg acagaagatt tatatgacta tattgacaaa
     1021 tacaacattg aattagatcc acgtttcaat gatatcttgg gcagacactc tcgaaagcga
     1081 tgggaacgct ttgtccacag tgaaaatcag caccttgtca gccctgaggc cttggatttc
     1141 ctggacaaac tgctgcgata tgaccaccag tcacggctta ctgcaagaga ggcaatggag
     1201 cacccctatt tctacactgt tgtgaaggac caggctcgaa tgggttcatc tagcatgcca
     1261 gggggcagta cgcccgtcag cagcgccaat atgatgtcag ggatttcttc agtgccaacc
     1321 ccttcacccc ttggacctct ggcaggctca ccagtgattg ctgctgccaa cccccttggg
     1381 atgcctgttc cagctgccgc tggcgctcag cagtacccat acgatgttcc tgactatgcg
     1441 ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt tccagattac
     1501 gcttaaaagc cgaattctgc agatatccat cacactggcg gccgctcgag catgcatcta
     1561 gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg
     1621 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     1681 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     1741 aggtgtcatt
//
