LOCUS       sequence-469783-        4697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of mouse Smad6 with stop codon (for native 
            or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4697)
  TITLE     Esposito lab plasmids 2025
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 8061-E36
FEATURES             Location/Qualifiers
     source          1..4697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2178..2277)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2294..2313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2295..2313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2318..2334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2318..2334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2765..3556
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3678..3787
                     /note="incP origin of transfer"
     rep_origin      4047..4635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4536..4555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgttcagg tctaaacgct cggggctggt
      721 gcggcgactt tggcgaagtc gtgtggtccc tgatcgggag gaaggcagcg gcggcggcgg
      781 tggtgtcgac gaggatggga gcctgggcag ccgagctgag cctgccccgc gggcacgaga
      841 gggcggaggc tgcagccgct ccgaagtccg ctcggtagcc ccgcggcggc cccgggacgc
      901 ggtgggaccg cgaggcgccg cgatcgcggg caggcgccgg cgcacagggg gcctcccgag
      961 gcccgtgtcg gagtcggggg ccggggctgg gggctccccg ctggatgtgg cggagcctgg
     1021 aggcccaggc tggctgcctg agagtgactg cgagacggtg acctgctgtc tcttctccga
     1081 acgggacgca gcaggcgcgc cccgggactc tggcgatccc caagccagac agtccccgga
     1141 gccggaggag ggcggcgggc ctcggagtcg cgaagcccgc tcgcgactgc tgcttctgga
     1201 gcaggagctc aagacggtca cgtactcgct gctcaagagg ctcaaggagc gttcgctgga
     1261 cacgctgttg gaggctgtgg agtcccgagg cggcgtaccg ggcggctgcg tgctggtgcc
     1321 gcgcgccgac ctccgcttgg gcggccagcc cgcgccaccg cagctgctgc tcggccgcct
     1381 cttccgctgg ccagacctgc agcacgcagt ggagctgaaa cccctgtgcg gctgccacag
     1441 ctttaccgcc gccgccgacg ggcccacggt gtgttgcaac ccctaccact tcagccggct
     1501 ctgcgggcca gaatcaccgc cgccccccta ttctcggctg tctcctcctg accagtacaa
     1561 gccactggat ctgtccgatt ctacattgtc ttacactgaa accgaggcca ccaactccct
     1621 catcactgct ccgggtgaat tctcagatgc cagcatgtct ccggatgcca ccaagccgag
     1681 ccactggtgc agcgtggcgt actgggagca ccggacacgc gtgggccgcc tctatgcggt
     1741 gtacgaccag gctgtcagca tcttctacga cctacctcag ggcagcggct tctgcctggg
     1801 ccagctcaac ctggagcagc gcagtgagtc ggtgcggcgc acgcgcagca agatcggttt
     1861 tggcatactg ctcagcaagg agccagacgg cgtgtgggcc tacaaccggg gcgagcaccc
     1921 catcttcgtc aactccccga cgctggatgc gcccggaggc cgcgccctgg tcgtgcgcaa
     1981 ggtgccaccg ggttactcca tcaaggtgtt cgactttgag cgctcatggc tgctgcagca
     2041 cgcagacgcc gctcacggcc cctacgaccc gcacagtgtg cgcatcagct tcgccaaggg
     2101 ctggggaccc tgctactcac gacagttcat cacctcctgc ccctgttggc tggagatcct
     2161 actcaacaac cacagataat caactttctt gtacaaagtt ggcattataa gaaagcattg
     2221 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2281 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2341 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2401 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     2461 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     2521 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     2581 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     2641 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     2701 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     2761 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     2821 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     2881 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     2941 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     3001 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     3061 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     3121 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     3181 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     3241 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     3301 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     3361 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3421 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3481 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     3541 ggtagtcggc aaataaccct cgactctaga ggatccccgg gtaccgagct cgaattccgt
     3601 gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc
     3661 tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag
     3721 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat
     3781 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa
     3841 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa
     3901 gcagggttat gcagcggaaa acggaattcg agctcggtac ccggggatcc tctagagtcg
     3961 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     4021 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4081 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4141 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4201 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4261 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4321 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4381 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4441 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4501 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4561 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4621 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4681 ccttttgctc acatgtt
//
