LOCUS       sequence-469799-        4896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ExE0582m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP14909 - pAAV-ExE0582m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             complement(236..247)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     RBS             658..669
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      832..841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             844..864
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1930..2041
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2159..2299
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2159..2288
                     /label=AAV2 ITR
     rep_origin      2374..2829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2461..2480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2671..2692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2846..2865)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2965..2987
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3025..3043)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3111..3215
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3216..3284,3285..4076)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3434..3453)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4247..4835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4736..4755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttgtca ctttgttact ctacagtgtt gcctggagtt
      181 cgatacttca ttactctata gtggggtgaa gaagttcacc ttcttatttt catttccttc
      241 cctcaatgat ttcttcagag ctagctctta ccagctagaa attcttcaaa cgacactcgt
      301 gccttccttc acacaggttg aactatttgt ctctaatgcc ctaaagtact ggtgttcaat
      361 cttccaggca cttccaatga tctgaaatct gacctgctta ggtcagctgg ctctgagatt
      421 atggtattct agtcctcaaa ccaacctgtt ggctcgttgg ttttgtacca aacacactga
      481 cttacatagc tcaaaatacc actggccttt taaaaatggc atatcacatt ccaggggagg
      541 atcaaaactg ctggctggtg atatttgtca agtctctcaa agttgcactt tccaggattt
      601 tcaattcact gaattcttag acagacatgt ttatgtgaaa gaattcttta tatatttttt
      661 ctcctctttg agtgggcaaa tgaaaatctt gacctctggg ttccttattt tatttgactc
      721 tctgtagtat ttaaatctta aaattttcct gagctcgggc tgggcataaa agtcagggca
      781 gagccatcta ttgcttacat ttgcttctgg gatccagatc tttcgaagct agccaccatg
      841 gtgcccaaga agaagaggaa agtctccaac ctgctgactg tgcaccaaaa cctgcctgcc
      901 ctccctgtgg atgccacctc tgatgaagtc aggaagaacc tgatggacat gttcagggac
      961 aggcaggcct tctctgaaca cacctggaag atgctcctgt ctgtgtgcag atcctgggct
     1021 gcctggtgca agctgaacaa caggaaatgg ttccctgctg aacctgagga tgtgagggac
     1081 tacctcctgt acctgcaagc cagaggcctg gctgtgaaga ccatccaaca gcacctgggc
     1141 cagctcaaca tgctgcacag gagatctggc ctgcctcgcc cttctgactc caatgctgtg
     1201 tccctggtga tgaggagaat cagaaaggag aatgtggatg ctggggagag agccaagcag
     1261 gccctggcct ttgaacgcac tgactttgac caagtcagat ccctgatgga gaactctgac
     1321 agatgccagg acatcaggaa cctggccttc ctgggcattg cctacaacac cctgctgcgc
     1381 attgccgaaa ttgccagaat cagagtgaag gacatctccc gcaccgatgg tgggagaatg
     1441 ctgatccaca ttggcaggac caagaccctg gtgtccacag ctggtgtgga gaaggccctg
     1501 tccctggggg ttaccaagct ggtggagaga tggatctctg tgtctggtgt ggctgatgac
     1561 cccaacaact acctgttctg ccgggtcaga aagaatggtg tggctgcccc ttctgccacc
     1621 tcccaactgt ccacccgggc cctggaaggg atctttgagg ccacccaccg cctgatctat
     1681 ggtgccaagg atgactctgg gcagagatac ctggcctggt ctggccactc tgccagagtg
     1741 ggtgctgcca ccgacatggc cagggctggt gtgtccatcc ctgaaatcat gcaggctggt
     1801 ggctggacca atgtgaacat tgtgatgaac tacatcagaa acctggactc tgagactggg
     1861 gccatggtga ggctgctcga agatggggac tgaggcgcgc cgaattcaag cttctcgaga
     1921 gatcttcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     1981 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     2041 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     2101 gggaggattg ggaagacaat agcaggcatg agatctcacg tgcggaccga gcggccgcag
     2161 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     2221 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     2281 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2341 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     2401 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     2461 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2521 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2581 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     2641 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     2701 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     2761 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     2821 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     2881 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     2941 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     3001 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     3061 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3121 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3181 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3241 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3301 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3361 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3421 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     3481 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3541 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3601 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3661 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3721 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     3781 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3841 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3901 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3961 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4021 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4081 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4141 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4201 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4261 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4321 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4381 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4441 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4501 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4561 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4621 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4681 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4741 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4801 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4861 ggttcctggc cttttgctgg ccttttgctc acatgt
//
