LOCUS       sequence-469812-       10312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to generate HA-tagged human ITGB6 stable 
            expressing cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Qiao W, Richards CM, Kim Y, Zengel JR, Ding S, Greenberg HB, Carette
            JE
  TITLE     MYADM binds human parechovirus 1 and is essential for viral entry.
  JOURNAL   Nat Commun. 2024 Apr 24;15(1):3469. doi: 10.1038/s41467-024-47825-0.
  PUBMED    38658526
REFERENCE   2  (bases 1 to 10312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-24"; volume: "15(1):3469. doi"; pages: " 
            10.1038/s41467-024-47825-0"
COMMENT     Sequence Label: plenti-ITGB6-HA
FEATURES             Location/Qualifiers
     source          1..10312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2998..3301
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3302..3505
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3456..3476
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3502..3526
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5973..5999
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6029..6617
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6082..6102)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6500..6511)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6631..7130
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6666..6689)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             7151..7750
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7151..7170)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7647..7667
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7888..8121
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8193..8314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8230..8249)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8284..8303
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8354..8489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8509..8528)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8510..8528)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8538..8555)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8538..8554)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8547..8569)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8696..9151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8783..8802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8993..9014
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9177..9281
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9282..9350,9351..10142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9500..9519)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg agttccgcgt
     3001 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3061 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3121 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3181 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3241 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3301 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     3361 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     3421 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     3481 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     3541 tccacgctgt tttgacctcc atagaagaca ccgactctag tccagtgtgg tggaattctg
     3601 cagataccat ggggattgaa ctgctttgcc tgttctttct atttctagga aggaatgatc
     3661 acgtacaagg tggctgtgcc ctgggaggtg cagaaacctg tgaagactgc ctgcttattg
     3721 gacctcagtg tgcctggtgt gctcaggaga attttactca tccatctgga gttggcgaaa
     3781 ggtgtgatac cccagcaaac cttttagcta aaggatgtca attaaacttc atcgaaaacc
     3841 ctgtctccca agtagaaata cttaaaaata agcctctcag tgtaggcaga cagaaaaata
     3901 gttctgacat tgttcagatt gcgcctcaaa gcttgatcct taagttgaga ccaggtggtg
     3961 cgcagactct gcaggtgcat gtccgccaga ctgaggacta cccggtggat ttgtattacc
     4021 tcatggacct ctccgcctcc atggatgacg acctcaacac aataaaggag ctgggctccc
     4081 ggctttccaa agagatgtct aaattaacca gcaactttag actgggcttc ggatcttttg
     4141 tggaaaaacc tgtatcccct ttcgtgaaaa caacaccaga agaaattgcc aacccttgca
     4201 gtagtattcc atacttctgt ttacctacat ttggattcaa gcacattttg ccattgacaa
     4261 atgatgctga aagattcaat gaaattgtga agaatcagaa aatttctgct aatattgaca
     4321 cacccgaagg tggatttgat gcaattatgc aagctgctgt gtgtaaggaa aaaattggct
     4381 ggcggaatga ctccctccac ctcctggtct ttgtgagtga tgctgattct cattttggaa
     4441 tggacagcaa actagcaggc atcgtcattc ctaatgacgg gctctgtcac ttggacagca
     4501 agaatgaata ctccatgtca actgtcttgg aatatccaac aattggacaa ctcattgata
     4561 aactggtaca aaacaacgtg ttattgatct tcgctgtaac ccaagaacaa gttcatttat
     4621 atgagaatta cgcaaaactt attcctggag ctacagtagg tctacttcag aaggactccg
     4681 gaaacattct ccagctgatc atctcagctt atgaagaact gcggtctgag gtggaactgg
     4741 aagtattagg agacactgaa ggactcaact tgtcatttac agccatctgt aacaacggta
     4801 ccctcttcca acaccaaaag aaatgctctc acatgaaagt gggagacaca gcttccttca
     4861 gcgtgactgt gaatatccca cactgcgaga gaagaagcag gcacattatc ataaagcctg
     4921 tggggctggg ggatgccctg gaattacttg tcagcccaga atgcaactgc gactgtcaga
     4981 aagaagtgga agtgaacagc tccaaatgtc accacgggaa cggctctttc cagtgtgggg
     5041 tgtgtgcctg ccaccctggc cacatggggc ctcgctgtga gtgtggcgag gacatgctga
     5101 gcacagattc ctgcaaggag gccccagatc atccctcctg cagcggaagg ggtgactgct
     5161 actgtgggca gtgtatctgc cacttgtctc cctatggaaa catttatggg ccttattgcc
     5221 agtgtgacaa tttctcctgc gtgagacaca aagggctgct ctgcggaggt aacggcgact
     5281 gtgactgtgg tgaatgtgtg tgcaggagcg gctggactgg cgagtactgc aactgcacca
     5341 ccagcacgga ctcctgcgtc tctgaagatg gagtgctctg cagcgggcgc ggggactgtg
     5401 tttgtggcaa gtgtgtttgc acaaaccctg gagcctcagg accaacctgt gaacgatgtc
     5461 ctacctgtgg tgacccctgt aactctaaac ggagctgcat tgagtgccac ctgtcagcag
     5521 ctggccaagc ccgagaagaa tgtgtggaca agtgcaaact agctggtgcg accatcagtg
     5581 aagaagaaga tttctcaaag gatggttctg tttcctgctc tctgcaagga gaaaatgaat
     5641 gtcttattac attcctaata actacagata atgaggggaa aaccatcatt cacagcatca
     5701 atgaaaaaga ttgtccgaag cctccaaaca ttcccatgat catgttaggg gtttccctgg
     5761 ctattcttct catcggggtt gtcctactgt gcatctggaa gctactggtg tcatttcatg
     5821 atcgtaaaga agttgccaaa tttgaagcag aacgatcaaa agccaagtgg caaacgggaa
     5881 ccaatccact ctacagagga tccacaagta cttttaaaaa tgtaacttat aaacacaggg
     5941 aaaaacaaaa ggtagacctt tccacagatt gctatccgta tgatgttccg gattatgcat
     6001 aaatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag
     6061 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6121 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6181 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6241 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6301 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6361 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6421 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6481 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6541 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6601 ttgggccgcc tccccgcctg gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     6661 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     6721 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     6781 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     6841 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     6901 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     6961 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     7021 tcaggggcgg ggcgggcgcc cgaagggcct ccggaggccc ggcattctgc acgcttcaaa
     7081 aacgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     7141 caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     7201 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     7261 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     7321 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     7381 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     7441 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     7501 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     7561 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7621 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     7681 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     7741 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     7801 tgcatctcga gggcccggta cctttaagac caatgactta caaggcagct gtagatctta
     7861 gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag
     7921 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7981 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8041 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8101 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     8161 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     8221 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8281 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     8341 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8401 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8461 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     8521 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8581 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8641 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     8701 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8761 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8821 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8881 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8941 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9001 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9061 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9121 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     9181 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9241 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9301 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9361 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9421 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9481 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9541 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9601 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9661 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9721 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9781 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9841 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9901 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9961 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10021 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10081 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10141 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10201 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10261 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
