LOCUS       sequence-469821-        6851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of bovine arrestin-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Pan L, Gurevich EV, Gurevich VV
  TITLE     The nature of the arrestin x receptor complex determines the 
            ultimate fate of the internalized receptor.
  JOURNAL   J Biol Chem. 2003 Mar 28;278(13):11623-32. doi: 
            10.1074/jbc.M209532200. Epub 2003 Jan 13.
  PUBMED    12525498
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M209532200"; journalName: "J Biol Chem"; date: 
            "2003-03-28- 28"; volume: "278"; issue: "13"; pages: "11623-32"
COMMENT     Sequence Label: p3A2
FEATURES             Location/Qualifiers
     source          1..6851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      495..1083
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     984..1003
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1237..1254
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1371..1392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1407..1412,1413..1430,1431..1437)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1445..1461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1450..1472
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1469..1485
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1469..1485
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1534..1655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1545..1564)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1599..1618
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1829..2623)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1940..1959)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2550..2569
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2690..3019)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2704..2839
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2758..2777)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3004..3024
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3033..3461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3143..3164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3355..3374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3507..3731
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3720..3737
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        3757..3775
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(5188..5206)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5297..5316)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5298..5316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5361..5564)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5390..5410)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5565..5944
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6116..6135
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6210..6314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6315..6383,6384..6851,1..324)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6533..6552)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
       61 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
      121 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
      181 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
      241 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
      301 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
      361 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
      421 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
      481 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
      541 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      601 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      661 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      721 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      781 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      841 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      901 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      961 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1021 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     1081 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     1141 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1201 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     1261 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     1321 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     1381 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     1441 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     1501 ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag tttggacaaa
     1561 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     1621 tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa ctccagcatg
     1681 agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac
     1741 ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg
     1801 tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     1861 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     1921 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     1981 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     2041 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct
     2101 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     2161 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     2221 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     2281 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     2341 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     2401 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac
     2461 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     2521 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     2581 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     2641 ctgtctcttg atcagatccg aaaatggata tacaagctcc cgggagcttt ttgcaaaagc
     2701 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggca gaggcggcct
     2761 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     2821 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     2881 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
     2941 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     3001 cacaccctaa ctgacacaca ttccacagaa ttaattcgcg ttaaattttt gttaaatcag
     3061 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     3121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     3241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     3361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tggggatacc ccctagagcc
     3481 ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag catgcctgct
     3541 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
     3601 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
     3661 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
     3721 agaaggcaca gtcgaggctg atcagcgagc tctagcattt aggtgacact atagaatagg
     3781 gccccctggt agaaactgga agaacaaagg ggagggggag ccaggacagg aagaagacga
     3841 acaagcatcc tagtgcatga gagtggacct ggagctgccc ggagggaggg cagccccagt
     3901 ctatctgtcg ttgagccgcg gagagccggt accatcctcc tcttcctcct tgtcatcctt
     3961 catgcctttt agtctctggc gggcaaagtc ctcaaacaca atgtcgtcat cgttggtgtc
     4021 aagttctatg agattggtat ctaccggcgt ctcgtgctct ggaacttccc ggtgtggggg
     4081 ttcctctttg ggcttggggt gcattagggt gaaaggcagt tccacggcca catcactgga
     4141 tgcaagatct cccaacaggc cgccacgaga caccaccagc ttcactttca ctttgtagga
     4201 aacaatgatg cccaggatct cccggttggc tccttccctc aacagggtgc tggaggccag
     4261 gttcgtgtcc tcgtgtttga gcttcccgtc cagggcgagg ccccgcttct ctcgattgtt
     4321 ggccaggaag ggggtcagcg tgtagacctt gcagaacgta gagctgggtg ccactgtgtc
     4381 atcagcctct tccatggcca cagggcactt gtactgggct gtgttgaaca gacagatgtc
     4441 tgcatactgg cgcaccgaga tcttgatctt cttcaccgtc ttgttggtgt tgttggtgac
     4501 atggacgttg acactgatgg gttctccgtg gtagtagatc tccttgtcca gggaggcctc
     4561 cagatgcaag ggcttgtctg acatgaggaa ctgcctggtg gtctcggccg tgggctgggg
     4621 gccaggcctc tctggggcat actgaacctt ccggatgacc aggcgcacag aattccgctt
     4681 gtggatcttc tcctccaggt tctccgcaca gaaggctttc acttcgtagt ccacaccgca
     4741 ggccttccct gtatcttcag gtcccggctg caaagtcaca gagcatggga ggttcggagg
     4801 gatctcaaag gtgaaagggt aggcatgctc gcccagcttc ttgatgaggc gctcctgcag
     4861 ccgcgtcagg ggcttcttgt cctcaggggc cggcgggaaa gactgcacgt tggccacaaa
     4921 caggtccttg cgaaaggtca ggcccaggac atccaggtcc tcccggccgt agcggaaggc
     4981 gcaggtcagc gtcacataga ctctcctctc cttgagatac tccggatcca caagaaccac
     5041 tccatccacg ggctccacga ggtcgatgtg gtccacaaag tcccgctttc ccagatagac
     5101 ggtgagcttt ccattggggc tcgccttctt gaacacccgc gtccctttgt cgcccatggt
     5161 ggtttaaaaa aaaaagaatt cttgtattct atagtgtcac ctaaatcgcg gccgccagtg
     5221 tgatggatat ctgcagaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg
     5281 taccaagctt gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg
     5341 ggttctctag ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg
     5401 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
     5461 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
     5521 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
     5581 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     5641 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     5701 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     5761 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     5821 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     5881 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
     5941 tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc
     6001 agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg
     6061 cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa ctatgcggca
     6121 tcagagcaga ttgtactgag agtgcaccat aggggatcgg gagatctccc gatccgtcga
     6181 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6241 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6301 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6361 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6421 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6481 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6541 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6601 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6661 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6721 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6781 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6841 gtgacaccac g
//
