LOCUS       sequence-469843-       11214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with mobile gRNAs targeting SlHAIRPLUS and 
            SlAN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Liu D, Ellison EE, Myers EA, Donahue LI, Xuan S, Swanson R, Qi S, 
            Prichard LE, Starker CG, Voytas DF
  TITLE     Heritable gene editing in tomato through viral delivery of 
            isopentenyl transferase and single-guide RNAs to latent axillary 
            meristematic cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Sep 24;121(39):e2406486121. doi: 
            10.1073/pnas.2406486121. Epub 2024 Sep 16.
  PUBMED    39284063
REFERENCE   2  (bases 1 to 11214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-09-24"; volume: "121(39):e2406486121. 
            doi"; pages: " 10.1073/pnas.2406486121. Epub 2024 Sep 16"
COMMENT     Sequence Label: pDL639
FEATURES             Location/Qualifiers
     source          1..11214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        250..595
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     538..558
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2967..2984
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3019..3271
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3293..3317
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4618..5247
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5676..6749
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6815..7009
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7245..7267)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7353..7493
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7408..7427
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7508..7525)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7679..8267)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7759..7778)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9692..10486)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10911..10935
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtagaaaagg aagatggctt ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt
       61 caagatgcct ctaccgacag tggtcccaaa gatggacccc cacccacgag gaacatcgtg
      121 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tggtcaacat
      181 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      241 gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      301 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct tctacaaatg
      361 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca gtggtcccaa
      421 agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      481 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      541 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggataaaaca
      601 ttgcacctat ggtgttgccc tggctggggt atgtcagtga tcgcagtaga atgtactaat
      661 tgacaagttg gagaatacgg tagaacgtcc ttatccaaca cagcctttat ccctctccct
      721 gacgaggttt ttgtcagtgt aatatttctt tttgaactat ccagcttagt accgtacggg
      781 aaagtgactg gtgtgcttat ctttgaaatg ttactttggg tttcggttct ttaggttagt
      841 aagaaagcac ttgtcttctc atacaaagga aaacctgaga cgtatcgctt acgaaagtag
      901 caatgaaaga aaggtggtgg ttttaatcgc taccgcaaaa acgatggggt cgttttaatt
      961 aacttctcct acgcaagcgt ctaaacggac gttggggttt tgctagtttc tttagagaaa
     1021 actagctaag tctttaatgt tatcattaga gatggcataa atataatact tgtgtctgct
     1081 gataagatca ttttaatttg gacgattaga cttgttgaac tacaggttac tgaatcactt
     1141 gcgctaatca acatgggaga tatgtacgat gaatcatttg acaagtcggg cggtcctgct
     1201 gacttgatgg acgattcttg ggtggaatca gtttcgtgga aagatctgtt gaagaagtta
     1261 cacagcataa aatttgcact acagtctggt agagatgaga tcactgggtt actagcggca
     1321 ctgaatagac agtgtcctta ttcaccatat gagcagtttc cagataagaa ggtgtatttc
     1381 cttttagact cacgggctaa cagtgctctt ggtgtgattc agaacgcttc agcgttcaag
     1441 agacgagctg atgagaagaa tgcagtggcg ggtgttacaa atattcctgc gaatccaaac
     1501 acaacggtta cgacgaacca agggagtact actactacca aggcgaacac tggctcgact
     1561 ttggaagaag acttgtacac ttattacaaa ttcgatgatg cctctacagc tttccacaaa
     1621 tctctaactt cgttagagaa catggagttg aagagttatt accgaaggaa ctttgagaaa
     1681 gtattcggga ttaagtttgg tggagcagct gctagttcat ctgcaccgcc tccagcgagt
     1741 ggaggtccga tacgtcctaa tccctaggga tttaaggacg tgaactctgt tgagatctct
     1801 gtgaaattca gagggtgggt gataccatat tcactgatgc cattagcgac atctaaatag
     1861 ggctaattgt gactaatttg agggaatttc ctttaccatt gacgtcagtg tcgttggtag
     1921 catttgagtt tcggagcatc ttgttctggg gtttcacact atctttagag aaagtgttaa
     1981 gttaattaag ttatcttaat taagagcata attatactga tttgtctctc gttgatagag
     2041 tctatcattc tgttactaaa aatttgacaa ctcggtttgc tgacctactg gttactgtat
     2101 cacttacccg agttaacgag tctagaaacg agcatgcatc gtggcaatgg tttaagagct
     2161 atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg
     2221 gcaccgagtc ggtgctcccg ctcccgtagc tcagttggtt agagcgttgg tcttatgagc
     2281 cgaaggtcgc gggttcgagc cccgccggaa gcatgacgat taagcataag agcatcggtt
     2341 taagagctat gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg
     2401 aaaaagtggc accgagtcgg tgctcccgct cccgtagctc agttggttag agcgttggtc
     2461 ttatgagccg aaggtcgcgg gttcgagccc cgccggaagc aagtgccggg catgtcccga
     2521 agacattaaa ctacggttct ttaagtagat ccgtgtctga agttttaggt tcaatttaaa
     2581 cctacgagat tgacattctc gactgatctt gattgatcgg taagtctttt gtaatttaat
     2641 tttctttttg attttatttt aaattgttat ctgtttctgt gtatagactg tttgagatcg
     2701 gcgtttggcc gactcattgt cttaccatag gggaacggac tttgtttgtg ttgttatttt
     2761 atttgtattt tattaaaatt ctcaacgatc tgaaaaagcc tcgcggctaa gagattgttg
     2821 gggggtgagt aagtactttt aaagtgatga tggttacaaa ggcaaaaggg gtaaaacccc
     2881 tcgcctacgt aagcgttatt acgcccgtct gtacttatat cagtacactg acgagtccct
     2941 aaaggacgaa acgggagaac gctagccacc accaccacca ccacgtgtga attacaggtg
     3001 accagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     3061 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     3121 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     3181 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     3241 tcgcgcgcgg tgtcatctat gttactagat cgggaattaa actatcagtg tttgacagga
     3301 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
     3361 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
     3421 ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
     3481 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
     3541 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga
     3601 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc
     3661 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc
     3721 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc
     3781 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc
     3841 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg
     3901 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc
     3961 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc
     4021 gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta
     4081 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg
     4141 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc
     4201 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat
     4261 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa
     4321 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat
     4381 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga
     4441 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
     4501 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
     4561 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
     4621 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
     4681 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
     4741 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
     4801 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
     4861 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
     4921 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
     4981 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
     5041 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
     5101 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
     5161 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
     5221 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
     5281 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
     5341 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
     5401 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
     5461 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
     5521 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
     5581 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgcctg ccggccctgc
     5641 aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc
     5701 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
     5761 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
     5821 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
     5881 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
     5941 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
     6001 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
     6061 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
     6121 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
     6181 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
     6241 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
     6301 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
     6361 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
     6421 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
     6481 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
     6541 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
     6601 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
     6661 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
     6721 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
     6781 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc
     6841 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
     6901 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
     6961 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
     7021 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
     7081 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
     7141 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
     7201 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     7261 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     7321 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     7381 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     7441 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     7501 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7561 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7621 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7681 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7741 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7801 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7861 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7921 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7981 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8041 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8101 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8161 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8221 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8281 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg ctgatgaatc
     8341 ccctaatgat ttttatcaaa atcattaagt taaggtagat acacatcttg tcatatgatc
     8401 aaatggtttc gccaaaaatc aataatcaga caacaaaatg tgcgaactcg atattttaca
     8461 cgactctctt taccaattct gccccgaatt acacttaaaa cgactcaaca gcttaacgtt
     8521 ggcttgccac gccttacttg actgtaaaac tctcactctt accgaacttg gccgtaacct
     8581 gccaaccaaa gcgagaacaa aacataacat caaacgaatc gaccgattgt taggtaatcg
     8641 tcacctccac aaagagcgac tcgctgtata ccgttggcat gctagcttta tctgttcggg
     8701 caatacgatg cccattgtac ttgttgactg gtctgatatc cgtgagcaaa aacggcttat
     8761 ggtattgcga gcttcagtcg cactacacgg tcgttctgtt actctttatg agaaagcgtt
     8821 cccgctttca gagcaatgtt caaagaaagc tcatgaccaa tttctagccg accttgcgag
     8881 cattctaccg agtaacacca caccgctcat tgtcagtgat gctggcttta aagtgccatg
     8941 gtataaatcc gttgagaagc tgggttggta ctggttaagt cgagtaagag gaaaagtaca
     9001 atatgcagac ctaggagcgg aaaactggaa acctatcagc aacttacatg atatgtcatc
     9061 tagtcactca aagactttag gctataagag gctgactaaa agcaatccaa tctcatgcca
     9121 aattctattg tataaatctc gctctaaagg ccgaaaaaat cagcgctcga cacggactca
     9181 ttgtcaccac ccgtcaccta aaatctactc agcgtcggca aaggagccat ggattctagc
     9241 aactaactta cctgttgaaa ttcgaacacc caaacaactt gttaatatct attcgaagcg
     9301 aatgcagatt gaagaaacct tccgagactt gaaaagtcct gcctacggac taggcctacg
     9361 ccatagccga acgagcagct cagagcgttt tgatatcatg ctgctaatcg ccctgatgct
     9421 tcaactaaca tgttggcttg cgggcgttca tgctcagaaa caaggttggg acaagcactt
     9481 ccaggctaac acagtcagaa atcgaaacgt actctcaaca gttcgcttag gcatggaagt
     9541 tttgcggcat tctggctaca caataacaag ggaagactca ctcgtggctg caaccctgct
     9601 tactcaaaat ctattcacac atggttacgt tttggggaaa ttatgagggg atctctcagc
     9661 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat
     9721 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
     9781 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
     9841 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
     9901 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
     9961 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
    10021 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
    10081 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
    10141 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
    10201 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
    10261 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
    10321 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
    10381 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
    10441 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
    10501 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
    10561 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
    10621 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
    10681 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
    10741 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
    10801 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
    10861 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
    10921 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
    10981 atgtactgaa ttaacgccga attaattcct aggccaccat gttgggcccg gcgcgccaag
    11041 cttgcatgcc tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc
    11101 aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg
    11161 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gaca
//
