LOCUS       sequence-469889-       12421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ISYmu1 PDS3 gRNA12 TRV2 Architecture_B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, 
            Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, 
            Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE
  TITLE     Viral delivery of an RNA-guided genome editor for transgene-free 
            germline editing in Arabidopsis.
  JOURNAL   Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9.
  PUBMED    40263581
REFERENCE   2  (bases 1 to 12421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2025 Apr 22. doi: 10.1038/s41477-025-01989-9."
COMMENT     Sequence Label: pTW2082
FEATURES             Location/Qualifiers
     source          1..12421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1732..1756
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(1778..2030)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2065..2082)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     ncRNA           complement(2615..2682)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             complement(4010..4030)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4037..4084)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     promoter        complement(5661..6006)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5698..5718)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6535..6559
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6984..7778
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9203..9791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9692..9711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9945..9962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9977..10117
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10043..10062)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10203..10225
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10461..10655
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10721..11794)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(join(12223..12421,1..431))
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
       61 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
      121 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
      181 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
      241 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
      301 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
      361 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
      421 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
      481 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
      541 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
      601 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
      661 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
      721 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
      781 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
      841 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
      901 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
      961 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
     1021 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
     1081 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
     1141 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
     1201 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
     1261 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
     1321 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
     1381 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
     1441 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
     1501 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
     1561 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg
     1621 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
     1681 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg
     1741 ccaatatatc ctgtcaaaca ctgatagttt aattcccgat ctagtaacat agatgacacc
     1801 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     1861 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     1921 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     1981 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc
     2041 gagctggtca cctgtaattc acacgtggtg gtggtggtgg tggctagcgt tctcccgttt
     2101 cgtcctttag ggactcgtca gtgtactgat ataagtacag acgggcgtaa taacgcttac
     2161 gtaggcgagg ggttttaccc cttttgcctt tgtaaccatc atcactttaa aagtacttac
     2221 tcacccccca acaatctctt agccgcgagg ctttttcaga tcgttgagaa ttttaataaa
     2281 atacaaataa aataacaaca caaacaaagt ccgttcccct atggtaagac aatgagtcgg
     2341 ccaaacgccg atctcaaaca gtctatacac agaaacagat aacaatttaa aataaaatca
     2401 aaaagaaaat taaattacaa aagacttacc gatcaatcaa gatcagtcga gaatgtcaat
     2461 ctcgtaggtt taaattgaac ctaaaacttc agacacggat ctacttaaag aaccgtagtt
     2521 taatgtcttc gggacatgcc cgggtgcttc cggcggggct cgaacccgcg accttcggct
     2581 cataagacca acgctctaac caactgagct acgggtccca ttcgccatgc cgaagcatgt
     2641 tgcccagccg gcgccagcga ggaggctggg accatgccgg ccttatatgc tccaacgctt
     2701 gaaactctca tgactaaagt cacgagattc ttgggaacag agcatactta ccggcttatt
     2761 tctttgccgg cagaggtttc tcttgtttac caagctaccc ccgcaattcc tgcggttcct
     2821 gtttgcttct gtattgctaa gaccccggag tcagagctct tatcctgagt ccctcagcca
     2881 gtatatttct tgctgcattg acgtcacggt catggtgggc atgacaaaca ggacaggtcc
     2941 actcccttac atggagaggc ttcttcctgt ccttgtggcc gcactctgag catatctgac
     3001 ttgacggaaa ccatctgctt atcctgatga cttcttttcc ataccaggaa gccttgtact
     3061 gcagtttcga tacaaggctc gtccatgata catcagagat gctttttgcc agtttatgat
     3121 tgcgcatcat gcccttaacg ttaaggtcct cgatacagat gatatcgtgg tttttgacta
     3181 tctctgtact gagtttgtta agatattctt ttcgctggtt tgcaaccttt tcataaagtc
     3241 tcgctacctt ccgcctttgc ttctgatagt tcctggcttc agaaagggaa atgccccttt
     3301 tctccgcagc aagtgcacgc cttgcaagct ttcgctgctc ccgtctaagc ctttcttcca
     3361 tttgcctggt aaaatgattg ttgtcatgcc tgcttccgtc tgacataacc gcaaagtcaa
     3421 tgattccgag gtcaattccg acagatgagt cagtctttgg caattcacag atctcctctt
     3481 cgcataggat cgatacgaag aatttcccgc tcgatttcct tcgtactgtg gcattcagta
     3541 tacggccttt catctcccta ctgtctgcaa acttgacaag gcccagctta ggcagcttca
     3601 gacaatttcc ggaaactgcg atgttgttgt tcgtatattg tgtcgtgtag ctttgcctcg
     3661 ggctcttctt gctcttgaac tggggatgct cgttctgtcc tttgaaaaag cgggagaagg
     3721 cgtccgacag gttacgaagc gaattctgga gcgagaactt atccacttca caaagccata
     3781 ctgtttccgg gtccctctta agctttgtca gaagtttgga gcatgcgtaa taagtcagac
     3841 cttttccggt ctcctcatat tccttgttcc aaagttccag gaaatggttg tatacatacc
     3901 ttgaactgcc tattgtcttg gcaatcagtg tttcctgctt cttatctgga tagatacggt
     3961 attcataggc tttatgctgc agcatagcag ctggaactcc gtggattcca acctttctct
     4021 tcttctttgg agccatctta tcatcatcat ccttgtaatc cttatcatca tcatccttgt
     4081 aatccatctc gttaactcgg gtaagtgata cagtgaccag taggtcagca aaccgagttg
     4141 tcaaattttt aataacagaa tgatagactc tatcaacgag agacaaatca gtataattat
     4201 gctcttaatt aagataactt aattaactta acactttctc taaagatagt gtgaaacccc
     4261 agaacaagat gctcagcctg ttcttgcgca tgtattacta cagcgttttt aaccttgtgt
     4321 gccgaaactc aaatgctacc aacgacactg acgtcaatgg taaaggaaat tccctcaaat
     4381 tagtcacaat tagccctatt tagatgtcgc taatggcatc agtgaatatg gtatcaccca
     4441 ccctctgaat ttcacagaga tctcaacaga gttcacgtcc ttaaatccct agggattagg
     4501 acgtatcgga cctccactcg ctggaggcgg tgcagatgaa ctagcagctg ctccaccaaa
     4561 cttaatcccg aatactttct caaagttcct tcggtaataa ctcttcaact ccatgttctc
     4621 taacgaagtt agagatttgt ggaaagctgt agaggcatca tcgaatttgt aataagtgta
     4681 caagtcttct tccaaagtcg agccagtgtt cgccttggta gtagtagtac tcccttggtt
     4741 cgtcgtaacc gttgtgtttg gattcgcagg aatatttgta acacccgcca ctgcattctt
     4801 ctcatcagct cgtctcttga acgctgaagc gttctgaatc acaccaagag cactgttagc
     4861 ccgtgagtct aaaaggaaat acaccttctt atctggaaac tgctcatatg gtgaataagg
     4921 acactgtcta ttcagtgccg ctagtaaccc agtgatctca tctctaccag actgtagtgc
     4981 aaattttatg ctgtgtaact tcttcaacag atctttccac gaaactgatt ccacccaaga
     5041 atcgtccatc aagtcagcag gaccgcccga cttgtcaaat gattcatcgt acatatctcc
     5101 catgttgatt agcgcaagtg attcagtaac ctgtagttca acaagtctaa tcgtccaaat
     5161 taaaatgatc ttatcagcag acacaagtat tatatttatg ccatctctaa tgataacatt
     5221 aaagacttag ctagttttct ctaaagaaac tagcaaaacc ccaacgtccg tttagacgct
     5281 tgcgtaggag aagttaatta aaacgacccc atcgtttttg cggtagcgat taaaaccacc
     5341 acctttcttt cattgctact ttcgtaagcg atacgtctca ggttttcctt tgtatgagaa
     5401 gacaagtgct ttcttactaa cctaaagaac cgaaacccaa agtaacattt caaagataag
     5461 cacaccagtc actttcccgt acggtactaa gctggatagt tcaaaaagaa atattacact
     5521 gacaaaaacc tcgtcaggga gagggataaa ggctgtgttg gataaggacg ttctaccgta
     5581 ttctccaact tgtcaattag tacattctac tgcgatcact gacatacccc agccagggca
     5641 acaccatagg tgcaatgttt tatcctctcc aaatgaaatg aacttcctta tatagaggaa
     5701 gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
     5761 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gttcctcgtg
     5821 ggtgggggtc catctttggg accactgtcg gtagaggcat cttgaacgat agcctttcct
     5881 ttatcgcaat gatggcattt gtagaagcca tcttcctttt ctactgtcct ttcgatgaag
     5941 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
     6001 gtctcaatag ccctctggtc ttctgagact gtatctttga tattcttgga gtagacgaga
     6061 gtgtcgtgct ccaccatgtt gaccatcaca tcaatccact tgctttgaag acgtggttgg
     6121 aacgtcttct ttttccacga tgttcctcgt gggtgggggt ccatctttgg gaccactgtc
     6181 ggtagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtagaagcc
     6241 atcttccttt tctactgtcc tttcgatgaa gtgacagata gctgggcaat ggaatccgag
     6301 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctctggt cttctgagac
     6361 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tgacctgcag
     6421 gcatgcaagc ttggcgcgcc gggcccaaca tggtggccta ggaattaatt cggcgttaat
     6481 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     6541 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     6601 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     6661 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     6721 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     6781 gttgctgcct gtgatcaccg cggtttcaaa atcggctccg tcgatactat gttatacgcc
     6841 aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg
     6901 aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt
     6961 agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac
     7021 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
     7081 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
     7141 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
     7201 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
     7261 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
     7321 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
     7381 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
     7441 atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg
     7501 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
     7561 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
     7621 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg
     7681 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
     7741 aaataaaata ttatatttta ctggatgaat tgttttagta cctagaatgc atgaccaaaa
     7801 tcccttaacg ctgagagatc ccctcataat ttccccaaaa cgtaaccatg tgtgaataga
     7861 ttttgagtaa gcagggttgc agccacgagt gagtcttccc ttgttattgt gtagccagaa
     7921 tgccgcaaaa cttccatgcc taagcgaact gttgagagta cgtttcgatt tctgactgtg
     7981 ttagcctgga agtgcttgtc ccaaccttgt ttctgagcat gaacgcccgc aagccaacat
     8041 gttagttgaa gcatcagggc gattagcagc atgatatcaa aacgctctga gctgctcgtt
     8101 cggctatggc gtaggcctag tccgtaggca ggacttttca agtctcggaa ggtttcttca
     8161 atctgcattc gcttcgaata gatattaaca agttgtttgg gtgttcgaat ttcaacaggt
     8221 aagttagttg ctagaatcca tggctccttt gccgacgctg agtagatttt aggtgacggg
     8281 tggtgacaat gagtccgtgt cgagcgctga ttttttcggc ctttagagcg agatttatac
     8341 aatagaattt ggcatgagat tggattgctt ttagtcagcc tcttatagcc taaagtcttt
     8401 gagtgactag atgacatatc atgtaagttg ctgataggtt tccagttttc cgctcctagg
     8461 tctgcatatt gtacttttcc tcttactcga cttaaccagt accaacccag cttctcaacg
     8521 gatttatacc atggcacttt aaagccagca tcactgacaa tgagcggtgt ggtgttactc
     8581 ggtagaatgc tcgcaaggtc ggctagaaat tggtcatgag ctttctttga acattgctct
     8641 gaaagcggga acgctttctc ataaagagta acagaacgac cgtgtagtgc gactgaagct
     8701 cgcaatacca taagccgttt ttgctcacgg atatcagacc agtcaacaag tacaatgggc
     8761 atcgtattgc ccgaacagat aaagctagca tgccaacggt atacagcgag tcgctctttg
     8821 tggaggtgac gattacctaa caatcggtcg attcgtttga tgttatgttt tgttctcgct
     8881 ttggttggca ggttacggcc aagttcggta agagtgagag ttttacagtc aagtaaggcg
     8941 tggcaagcca acgttaagct gttgagtcgt tttaagtgta attcggggca gaattggtaa
     9001 agagagtcgt gtaaaatatc gagttcgcac attttgttgt ctgattattg atttttggcg
     9061 aaaccatttg atcatatgac aagatgtgta tctaccttaa cttaatgatt ttgataaaaa
     9121 tcattagggg attcatcagc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9181 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9241 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9301 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     9361 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9421 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9481 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9541 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9601 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9661 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9721 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9781 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9841 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9901 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9961 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
    10021 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
    10081 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
    10141 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
    10201 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
    10261 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
    10321 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
    10381 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
    10441 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
    10501 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
    10561 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
    10621 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
    10681 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
    10741 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
    10801 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
    10861 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
    10921 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
    10981 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
    11041 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
    11101 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
    11161 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
    11221 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
    11281 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
    11341 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
    11401 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
    11461 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
    11521 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
    11581 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    11641 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    11701 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    11761 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
    11821 agtgccattg cagggccggc aggcaaccca gccgcttacg cctggccaac cgcccgttcc
    11881 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    11941 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    12001 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    12061 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    12121 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    12181 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    12241 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    12301 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    12361 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    12421 t
//
