LOCUS       sequence-469905-        2568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence for AEBP2 Isoform 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pCDS.AEBP2.I3
FEATURES             Location/Qualifiers
     source          1..2568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(933..967)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1038..1697)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1612..1631
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1879..2467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2368..2387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgtacacac gtcggtactc aagcataagc agtactataa tggatgtaga
       61 cagcacaatt tccagtgggc gttcaactcc agcaatgatg aatggacaag gaagcactac
      121 ttcttcaagc aaaaatattg cctataattg ttgttgggac cagtgccagg cttgcttcaa
      181 ctctagccca gacctggcag atcacatccg ttccatacat gtagatggtc agcgaggagg
      241 ggtatttgtt tgcttatgga aaggttgtaa agtatataac actccatcta ccagtcaaag
      301 ttggttacaa aggcatatgc tgacacacag tggagacaaa cctttcaagt gtgttgttgg
      361 tggctgcaat gccagctttg cttctcaggg agggctagct cgtcatgtac ccacacactt
      421 cagtcagcag aactcctcaa aagtttctag ccagccaaag gccaaagaag aatctccttc
      481 taaagctgga atgaacaaaa ggaggaaatt aaagaacaaa agacgacgct cattaccacg
      541 gccacatgat ttcttcgatg cacaaacact ggatgcgata agacatcgag ccatatgctt
      601 taacctctca gctcatatag aaagtttagg gaagggacac agtgttgttt ttcatagtac
      661 tgtaatagct aagagaaaag aagattctgg gaagatcaaa cttttgcttc attggatgcc
      721 tgaagacatt ctgcctgatg tgtgggtgaa tgaaagtgaa cgacatcagt taaaaactaa
      781 agtagttcat ttatcaaagc tacccaaaga tactgccttg cttttggacc caaacatata
      841 cagaacaatg ccgcagaaga ggttgaagag aactctgata agaaaagtgt tcaatttgta
      901 tttaagcaaa cagggatcct gagaccagac caataaaaaa cgcccggcgg caaccgagcg
      961 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     1021 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
     1081 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
     1141 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
     1201 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
     1261 gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
     1321 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
     1381 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
     1441 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc
     1501 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
     1561 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
     1621 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
     1681 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
     1741 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc
     1801 aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1861 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1921 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1981 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2041 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2101 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2161 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2221 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2281 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2341 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2401 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2461 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2521 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtag
//
