LOCUS       sequence-469909-       10186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a 
            endonuclease. Additionally, it contains a mutation in the luxR 
            gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Golla SA, Abo-Hashesh M, Gupta D, Liu Y, Mahadevan R
  TITLE     Model-Based Optimization of a qCRISPRi Circuit for Dynamic Control 
            of Metabolic Pathways.
  JOURNAL   ACS Synth Biol. 2025 Oct 17;14(10):3890-3898. doi: 
            10.1021/acssynbio.5c00095. Epub 2025 Sep 29.
  PUBMED    41021780
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5c00095"; journalName: "ACS Synth Biol"; date: 
            "2025-10-17- 17"; volume: "14"; issue: "10"; pages: "3890-3898"
COMMENT     Sequence Label: pQdCas12a.luxR(mut)-empty
FEATURES             Location/Qualifiers
     source          1..10186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      105..876
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2356..3150
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPVEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2410..2429)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3020..3039
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     RBS             4887..4898
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        8814..8848
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(8916..9212)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     9062..9084
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9076..9093
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9077..9093
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9103..9122
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9103..9121
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9169..9189)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9169..9187)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9208..9224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9208..9224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9221..9243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9232..9248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9256..9262,9263..9280,9281..9286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9301..9322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(join(9628..10186,1..104))
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGE*QLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 gcaataagcc cggcccacgc ctcatgcgct ttgcgttccg tttgcaccca gtgaccgggc
       61 ttgttcttgg cttgaatgcc gatttctctg gactgcgtgg ccatgcttat ctccatgcgg
      121 taggggtgcc gcacggttgc ggcaccatgc acaatcagct gcaacttttt ggcagcgcga
      181 caacaattat gcgttgcgta aaagtggcag tcaattacag attttcttta acctacgcaa
      241 tgagctattg cggggggtgc cgcaatgagc tgttgcgtac cccccttttt taagttgttg
      301 atttttaagt ctttcgcatt tcgccctata tctagttctt tggtgcccaa agaagggcac
      361 ccctgcgggg ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc
      421 cggggcttgt tgatcgactg cgcggccttc ggccttgccc aaggtggcgc tgcccccttg
      481 gaacccccgc actcgccgcc gtgaggctcg gggggcaggc gggcgggctt cgcccttcga
      541 ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt caaggccaag ccgctgcgcg
      601 gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc aaccggcaag cgaagcgcgc
      661 aggccgcagg ccggaggctt ttccccagag aaaattaaaa aaattgatgg ggcaaggccg
      721 caggccgcgc agttggagcc ggtgggtatg tggtcgaagg ctgggtagcc ggtgggcaat
      781 ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc agcttgtcca gcagggttgt
      841 ccacgggccg agcgaagcga gccagccggt ggccgctcgc ggccatcgtc cacatatcca
      901 cgggctggca agggagcgca gcgaccgcgc agggcgaagc ccggagagca agcccgtagg
      961 gcgccgcagc cgccgtaggc ggtcacgact ttgcgaagca aagtctagtg agtatactca
     1021 agcattgagt ggcccgccgg aggcaccgcc ttgcgctgcc cccgtcgagc cggttggaca
     1081 ccaaaaggga ggggcaggca tggcggcata cgcgatcatg cgatgcaaga agctggcgaa
     1141 aatgggcaac gtggcggcca gtctcaagca cgcctaccgc gagcgcgaga cgcccaacgc
     1201 tgacgccagc aggacgccag agaacgagca ctgggcggcc agcagcaccg atgaagcgat
     1261 gggccgactg cgcgagttgc tgccagagaa gcggcgcaag gacgctgtgt tggcggtcga
     1321 gtacgtcatg acggccagcc cggaatggtg gaagtcggcc agccaagaac agcaggcggc
     1381 gttcttcgag aaggcgcaca agtggctggc ggacaagtac ggggcggatc gcatcgtgac
     1441 ggccagcatc caccgtgacg aaaccagccc gcacatgacc gcgttcgtgg tgccgctgac
     1501 gcaggacggc aggctgtcgg ccaaggagtt catcggcaac aaagcgcaga tgacccgcga
     1561 ccagaccacg tttgcggctg ctgtggccga tctagggctg caacggggca tcgagggcag
     1621 caaggcacgt cacacgcgca ttcaggcgtt ctacgaggcc ctggagcggc caccagtggg
     1681 ccacgtcacc atcagcccgc aagcggtcga gccacgcgcc tatgcaccgc agggattggc
     1741 cgaaaagctg ggaatctcaa agcgcgttga gacgccggaa gccgtggccg accggctgac
     1801 aaaagcggtt cggcaggggt atgagcctgc cctacaggcc gccgcaggag cgcgtgagat
     1861 gcgcaagaag gccgatcaag cccaagagac ggcccgagac cttcgggagc gcctgaagcc
     1921 cgttctggac gccctggggc cgttgaatcg ggatatgcag gccaaggccg ccgcgatcat
     1981 caaggccgtg ggcgaaaagc tgctgacgga acagcgggaa gtccagcgcc agaaacaggc
     2041 ccagcgccag taggaacgcg ggcgcgcaca tttccccgaa aagtgccacc tgggatgaat
     2101 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
     2161 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
     2221 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
     2281 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatttgatca agagacagga
     2341 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     2401 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     2461 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     2521 gccctgaatg aactacagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     2581 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     2641 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgtcgagaa agtatccatc
     2701 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     2761 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     2821 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     2881 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     2941 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     3001 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     3061 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     3121 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     3181 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     3241 tggactagag aattcatcct gaacttatct agattaattt aagactgctt ttttaaactg
     3301 ttcattaata ggcatagaca atacaaccga tttagtatca cctaatacat gaatttcttt
     3361 gtctccaata cgatgacaag gaactttaat acgctttaaa aatcgctcta ttgctgttga
     3421 tgttactgtt acatattctg taataccttg actaacagcg tgtttatata tagcttcaaa
     3481 tagtttcatt gtaatttcac tagcagagtt atttatcttt gagctatttt tacctacagc
     3541 aaaacgactt aattcgacta tattaggatc tttgggagca ctctgttgac caagcaattc
     3601 aggaaaaaca cttttcagca tataatcacc tgttgtaggt aataaacgcc agcatccact
     3661 tacattttca gtatcatcac aagcataaat atattctgca tttgagttat catactcatc
     3721 tgattcaagg ttattttcta caactaagtc ccactcaagt ctttgcttaa acacttgata
     3781 acgaagactt agaatacctt tatactcctc cgatggaatt gccaaaaaat ccgatttttt
     3841 tatcattata gtcataccaa cctcccttgc gtttattcga ctataacaaa ccattttctt
     3901 gcgtaaacct gtacgatcct acaggtgctt atgttaagta attgtattcc cagcgataca
     3961 atagtgtgac aaaaatccaa tttattagaa tcaaatgtca atccattacc gttttaatga
     4021 tatataacac gcaaaacttg cgacaaacaa taggtaagga taaagagatg ggtatgaaaa
     4081 acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt ggcagcaata
     4141 atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac
     4201 tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaattcta gataattacc
     4261 ctaaaaaatg gaggcaatat tatggcgcgc taatttaata aaatatgatc ctatagtaga
     4321 ttattctaaa tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa
     4381 taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag
     4441 tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac attcagaaat
     4501 tgacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc
     4561 ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt
     4621 aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat
     4681 ttcaaaaata ttaggctgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat
     4741 gaaactcgat acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat
     4801 tgattggcca ttttttaaaa attaaagatc tacctgtagg atcgtacagg tttacgcaag
     4861 aaaatggttt gttatagtcg aataaaaaag aggagaaagg atctatgtca atttatcaag
     4921 aatttgttaa taaatatagt ttaagtaaaa ctctaagatt tgagttaatc ccacagggta
     4981 aaacacttga aaacataaaa gcaagaggtt tgattttaga tgatgagaaa agagctaaag
     5041 actacaaaaa ggctaaacaa ataattgata aatatcatca gttttttata gaggagatat
     5101 taagttcggt ttgtattagc gaagatttat tacaaaacta ttctgatgtt tattttaaac
     5161 ttaaaaagag tgatgatgat aatctacaaa aagattttaa aagtgcaaaa gatacgataa
     5221 agaaacaaat atctgaatat ataaaggact cagagaaatt taagaatttg tttaatcaaa
     5281 accttatcga tgctaaaaaa gggcaagagt cagatttaat tctatggcta aagcaatcta
     5341 aggataatgg tatagaacta tttaaagcca atagtgatat cacagatata gatgaggcgt
     5401 tagaaataat caaatctttt aaaggttgga caacttattt taagggtttt catgaaaata
     5461 gaaaaaatgt ttatagtagc aatgatattc ctacatctat tatttatagg atagtagatg
     5521 ataatttgcc taaatttcta gaaaataaag ctaagtatga gagtttaaaa gacaaagctc
     5581 cagaagctat aaactatgaa caaattaaaa aagatttggc agaagagcta acctttgata
     5641 ttgactacaa aacatctgaa gttaatcaaa gagttttttc acttgatgaa gtttttgaga
     5701 tagcaaactt taataattat ctaaatcaaa gtggtattac taaatttaat actattattg
     5761 gtggtaaatt tgtaaatggt gaaaatacaa agagaaaagg tataaatgaa tatataaatc
     5821 tatactcaca gcaaataaat gataaaacac tcaaaaaata taaaatgagt gttttattta
     5881 agcaaatttt aagtgataca gaatctaaat cttttgtaat tgataagtta gaagatgata
     5941 gtgatgtagt tacaacgatg caaagttttt atgagcaaat agcagctttt aaaacagtag
     6001 aagaaaaatc tattaaagaa acactatctt tattatttga tgatttaaaa gctcaaaaac
     6061 ttgatttgag taaaatttat tttaaaaatg ataaatctct tactgatcta tcacaacaag
     6121 tttttgatga ttatagtgtt attggtacag cggtactaga atatataact caacaaatag
     6181 cacctaaaaa tcttgataac cctagtaaga aagagcaaga attaatagcc aaaaaaactg
     6241 aaaaagcaaa atacttatct ctagaaacta taaagcttgc cttagaagaa tttaataagc
     6301 atagagatat agataaacag tgtaggtttg aagaaatact tgcaaacttt gcggctattc
     6361 cgatgatatt tgatgaaata gctcaaaaca aagacaattt ggcacagata tctatcaaat
     6421 atcaaaatca aggtaaaaaa gacctacttc aagctagtgc ggaagatgat gttaaagcta
     6481 tcaaggatct tttagatcaa actaataatc tcttacataa actaaaaata tttcatatta
     6541 gtcagtcaga agataaggca aatattttag acaaggatga gcatttttat ctagtatttg
     6601 aggagtgcta ctttgagcta gcgaatatag tgcctcttta taacaaaatt agaaactata
     6661 taactcaaaa gccatatagt gatgagaaat ttaagctcaa ttttgagaac tcgactttgg
     6721 ctaatggttg ggataaaaat aaagagcctg acaatacggc aattttattt atcaaagatg
     6781 ataaatatta tctgggtgtg atgaataaga aaaataacaa aatatttgat gataaagcta
     6841 tcaaagaaaa taaaggcgag ggttataaaa aaattgttta taaactttta cctggcgcaa
     6901 ataaaatgtt acctaaggtt ttcttttctg ctaaatctat aaaattttat aatcctagtg
     6961 aagatatact tagaataaga aatcattcca cacatacaaa aaatggtagt cctcaaaaag
     7021 gatatgaaaa atttgagttt aatattgaag attgccgaaa atttatagat ttttataaac
     7081 agtctataag taagcatccg gagtggaaag attttggatt tagattttct gatactcaaa
     7141 gatataattc tatagatgaa ttttatagag aagttgaaaa tcaaggctac aaactaactt
     7201 ttgaaaatat atcagagagc tatattgata gcgtagttaa tcagggtaaa ttgtacctat
     7261 tccaaatcta taataaagat ttttcagctt atagcaaagg gcgaccaaat ctacatactt
     7321 tatattggaa agcgctgttt gatgagagaa atcttcaaga tgtggtttat aagctaaatg
     7381 gtgaggcaga gcttttttat cgtaaacaat caatacctaa aaaaatcact cacccagcta
     7441 aagaggcaat agctaataaa aacaaagata atcctaaaaa agagagtgtt tttgaatatg
     7501 atttaatcaa agataaacgc tttactgaag ataagttttt ctttcactgt cctattacaa
     7561 tcaattttaa atctagtgga gctaataagt ttaatgatga aatcaattta ttgctaaaag
     7621 aaaaagcaaa tgatgttcat atattaagta tagctagagg tgaaagacat ttagcttact
     7681 atactttggt agatggtaaa ggcaatatca tcaaacaaga tactttcaac atcattggta
     7741 atgatagaat gaaaacaaac taccatgata agcttgctgc aatagagaaa gatagggatt
     7801 cagctaggaa agactggaaa aagataaata acatcaaaga gatgaaagag ggctatctat
     7861 ctcaggtagt tcatgaaata gctaagctag ttatagagta taatgctatt gtggtttttg
     7921 aggatttaaa ttttggattt aaaagagggc gtttcaaggt agagaagcag gtctatcaaa
     7981 agttagaaaa aatgctaatt gagaaactaa actatctagt tttcaaagat aatgagtttg
     8041 ataaaactgg gggagtgctt agagcttatc agctaacagc accttttgag acttttaaaa
     8101 agatgggtaa acaaacaggt attatctact atgtaccagc tggttttact tcaaaaattt
     8161 gtcctgtaac tggttttgta aatcagttat atcctaagta tgaaagtgtc agcaaatctc
     8221 aagagttctt tagtaagttt gacaagattt gttataacct tgataagggc tattttgagt
     8281 ttagttttga ttataaaaac tttggtgaca aggctgccaa aggcaagtgg actatagcta
     8341 gctttgggag tagattgatt aactttagaa attcagataa aaatcataat tgggatactc
     8401 gagaagttta tccaactaaa gagttggaga aattgctaaa agattattct atcgaatatg
     8461 ggcatggcga atgtatcaaa gcagctattt gcggtgagag cgacaaaaag ttttttgcta
     8521 agctaactag tgtcctaaat actatcttac aaatgcgtaa ctcaaaaaca ggtactgagt
     8581 tagattatct aatttcacca gtagcagatg taaatggcaa tttctttgat tcgcgacagg
     8641 cgccaaaaaa tatgcctcaa gatgctgatg ccaatggtgc ttatcatatt gggctaaaag
     8701 gtctgatgct actaggtagg atcaaaaata atcaagaggg caaaaaactc aatttggtta
     8761 tcaaaaatga agagtatttt gagttcgtgc agaataggaa taactaactc gagttgacag
     8821 ctagctcagt cctaggtata atgctagcgc tgatttaggc aaaaacgggt ctaagaactt
     8881 taaataattt ctactgttgt agatgagagc aggtgtcagc cgctacaggg cgcgtcccat
     8941 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     9001 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     9061 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     9121 ggcgaattgg gtaccgggcc ccccctcgag gtcctccagc ttttgttccc tttagtgagg
     9181 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9241 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9301 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc acccacctgc
     9361 aaaagtctaa gaactttaaa taatttctac tgttgtagat gctactattc ctgtgccttc
     9421 agataattca gtctaagaac tttaaataat ttctactgtt gtagatgtct agagcctttt
     9481 gtattagtag ccggtctaag aactttaaat aatttctact gttgtagatt agcgatttat
     9541 gaaggtcatt ttttttgtta acttcaggac ctgcagtcta gtatagtctg gaacagcgca
     9601 cttacgggtt gctgcgcaac ccaagtgcta ccggcgcggc agcgtgaccc gtgtcggcgg
     9661 ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg ggcatcggca ggcgctgctg
     9721 cccgcgccgt tcccattcct ccgtttcggt caaggctggc aggtctggtt ccatgcccgg
     9781 aatgccgggc tggctgggcg gctcctcgcc ggggccggtc ggtagttgct actcgcccgg
     9841 atacagggtc gggatgcggc gcaggtcgcc atgccccaac agcgattcgt cctggtcgtc
     9901 gtgatcaacc accacggcgg cactgaacac cgacaggcgc aactggtcgc ggggctggcc
     9961 ccacgccacg cggtcattga ccacgtaggc cgacacggtg ccggggccgt tgagcttcac
    10021 gacggagatc cagcgctcgg ccaccaagtc cttgactgcg tattggaccg tccgcaaaga
    10081 acgtccgatg agcttggaaa gtgtcttctg gctgaccacc acggcgttct ggtggcccat
    10141 ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt ttcctc
//
