LOCUS       sequence-469921-        9895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split NanoLuc luciferase biosensor fused to dSpCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9895)
  TITLE     A Split Luciferase Biosensing Platform for Detection and Imaging of 
            Chromatin Loops in Individual Live Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: dSpCas9-SmBiT
FEATURES             Location/Qualifiers
     source          1..9895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1211..1230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1402..1781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1782..1985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1936..1956
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1982..2006
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2103..2123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2142..2207
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(2292..2318,2319..2321,2322..4805,4806..4811,4812..4877,
                     4878..4880,4881..6395)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGDTAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2292..6395
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGDTAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6504..6551
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(6633..6652)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6677..6725
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6927..7355
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7014..7033)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7224..7245
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7364..7384)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7369..7698
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7549..7684
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7611..7630
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7765..8559
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7819..7838)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8429..8448
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8735..8856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8772..8791)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8826..8845
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8905..8921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8905..8921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8918..8940)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8929..8945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8953..8959,8960..8977,8978..8983))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8998..9019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9136..9153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9307..9895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9387..9406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1381 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     1681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1981 gagctcgttt agtgaaccgt cagatcgcct ggacacgcca tccacgctgt tttgacctcc
     2041 atagaagaca ccgggaccga tccagcctcc ggactctaga ggatcgaacc cttgccacca
     2101 tgcccaagaa gaagaggaag gtgggaggct ccggaggaag cgactataag gaccacgacg
     2161 gagactacaa ggatcatgat attgattaca aagacgatga cgataagggt ggcgggtccg
     2221 gcggtggatc cggtaccgga gggagtggtg gaagcggcgg ttctggtggc tcaggcggca
     2281 gcggccggcc aatggacaag aagtactcca ttgggctcgc tatcggcaca aacagcgtcg
     2341 gctgggccgt cattacggac gagtacaagg tgccgagcaa aaaattcaaa gttctgggca
     2401 ataccgatcg ccacagcata aagaagaacc tcattggcgc cctcctgttc gactccgggg
     2461 acacggccga agccacgcgg ctcaaaagaa cagcacggcg cagatatacc cgcagaaaga
     2521 atcggatctg ctacctgcag gagatcttta gtaatgagat ggctaaggtg gatgactctt
     2581 tcttccatag gctggaggag tcctttttgg tggaggagga taaaaagcac gagcgccacc
     2641 caatctttgg caatatcgtg gacgaggtgg cgtaccatga aaagtaccca accatatatc
     2701 atctgaggaa gaagcttgta gacagtactg ataaggctga cttgcggttg atctatctcg
     2761 cgctggcgca tatgatcaaa tttcggggac acttcctcat cgagggggac ctgaacccag
     2821 acaacagcga tgtcgacaaa ctctttatcc aactggttca gacttacaat cagcttttcg
     2881 aagagaaccc gatcaacgca tccggagttg acgccaaagc aatcctgagc gctaggctgt
     2941 ccaaatcccg gcggctcgaa aacctcatcg cacagctccc tggggagaag aagaacggcc
     3001 tgtttggtaa tcttatcgcc ctgtcactcg ggctgacccc caactttaaa tctaacttcg
     3061 acctggccga agatgccaag cttcaactga gcaaagacac ctacgatgat gatctcgaca
     3121 atctgctggc ccagatcggc gaccagtacg cagacctttt tttggcggca aagaacctgt
     3181 cagacgccat tctgctgagt gatattctgc gagtgaacac ggagatcacc aaagctccgc
     3241 tgagcgctag tatgatcaag cgctatgatg agcaccacca agacttgact ttgctgaagg
     3301 cccttgtcag acagcaactg cctgagaagt acaaggaaat tttcttcgat cagtctaaaa
     3361 atggctacgc cggatacatt gacggcggag caagccagga ggaattttac aaatttatta
     3421 agcccatctt ggaaaaaatg gacggcaccg aggagctgct ggtaaagctt aacagagaag
     3481 atctgttgcg caaacagcgc actttcgaca atggaagcat cccccaccag attcacctgg
     3541 gcgaactgca cgctatcctc aggcggcaag aggatttcta cccctttttg aaagataaca
     3601 gggaaaagat tgagaaaatc ctcacatttc ggatacccta ctatgtaggc cccctcgccc
     3661 ggggaaattc cagattcgcg tggatgactc gcaaatcaga agagaccatc actccctgga
     3721 acttcgagga agtcgtggat aagggggcct ctgcccagtc cttcatcgaa aggatgacta
     3781 actttgataa aaatctgcct aacgaaaagg tgcttcctaa acactctctg ctgtacgagt
     3841 acttcacagt ttataacgag ctcaccaagg tcaaatacgt cacagaaggg atgagaaagc
     3901 cagcattcct gtctggagag cagaagaaag ctatcgtgga cctcctcttc aagacgaacc
     3961 ggaaagttac cgtgaaacag ctcaaagaag actatttcaa aaagattgaa tgtttcgact
     4021 ctgttgaaat cagcggagtg gaggatcgct tcaacgcatc cctgggaacg tatcacgatc
     4081 tcctgaaaat cattaaagac aaggacttcc tggacaatga ggagaacgag gacattcttg
     4141 aggacattgt cctcaccctt acgttgtttg aagataggga gatgattgaa gaacgcttga
     4201 aaacttacgc tcatctcttc gacgacaaag tcatgaaaca gctcaagagg cgccgatata
     4261 caggatgggg gcggctgtca agaaaactga tcaatgggat ccgagacaag cagagtggaa
     4321 agacaatcct ggattttctt aagtccgatg gatttgccaa caggaacttc atgcagttga
     4381 tccatgatga ctctctcacc tttaaggagg acatccagaa agcacaagtt tctggccagg
     4441 gggacagtct tcacgagcac atcgctaatc ttgcaggtag cccagctatc aaaaagggaa
     4501 tactgcagac cgttaaggtc gtggatgaac tcgtcaaagt aatgggaagg cataagcccg
     4561 agaatatcgt tatcgagatg gcccgagaga accaaactac ccagaaggga cagaagaaca
     4621 gtagggaaag gatgaagagg attgaagagg gtataaaaga actggggtcc caaatcctta
     4681 aggaacaccc agttgaaaac acccagcttc agaatgagaa gctctacctg tactacctgc
     4741 agaacggcag ggacatgtac gtggatcagg aactggacat caatcggctc tccgactacg
     4801 acgtggatgc tatcgtgccc cagtcttttc tcaaagatga ttctattgat aataaagtgt
     4861 tgacaagatc cgataaaaat agagggaaga gtgataacgt cccctcagaa gaagttgtca
     4921 agaaaatgaa aaattattgg cggcagctgc tgaacgccaa actgatcaca caacggaagt
     4981 tcgataatct gactaaggct gaacgaggtg gcctgtctga gttggataaa gccggcttca
     5041 tcaaaaggca gcttgttgag acacgccaga tcaccaagca cgtggcccaa attctcgatt
     5101 cacgcatgaa caccaagtac gatgaaaatg acaaactgat tcgagaggtg aaagttatta
     5161 ctctgaagtc taagctggtc tcagatttca gaaaggactt tcagttttat aaggtgagag
     5221 agatcaacaa ttaccaccat gcgcatgatg cctacctgaa tgcagtggta ggcactgcac
     5281 ttatcaaaaa atatcccaag cttgaatctg aatttgttta cggagactat aaagtgtacg
     5341 atgttaggaa aatgatcgca aagtctgagc aggaaatagg caaggccacc gctaagtact
     5401 tcttttacag caatattatg aattttttca agaccgagat tacactggcc aatggagaga
     5461 ttcggaagcg accacttatc gaaacaaacg gagaaacagg agaaatcgtg tgggacaagg
     5521 gtagggattt cgcgacagtc cggaaggtcc tgtccatgcc gcaggtgaac atcgttaaaa
     5581 agaccgaagt acagaccgga ggcttctcca aggaaagtat cctcccgaaa aggaacagcg
     5641 acaagctgat cgcacgcaaa aaagattggg accccaagaa atacggcgga ttcgattctc
     5701 ctacagtcgc ttacagtgta ctggttgtgg ccaaagtgga gaaagggaag tctaaaaaac
     5761 tcaaaagcgt caaggaactg ctgggcatca caatcatgga gcgatcaagc ttcgaaaaaa
     5821 accccatcga ctttctcgag gcgaaaggat ataaagaggt caaaaaagac ctcatcatta
     5881 agcttcccaa gtactctctc tttgagcttg aaaacggccg gaaacgaatg ctcgctagtg
     5941 cgggcgagct gcagaaaggt aacgagctgg cactgccctc taaatacgtt aatttcttgt
     6001 atctggccag ccactatgaa aagctcaaag ggtctcccga agataatgag cagaagcagc
     6061 tgttcgtgga acaacacaaa cactaccttg atgagatcat cgagcaaata agcgaattct
     6121 ccaaaagagt gatcctcgcc gacgctaacc tcgataaggt gctttctgct tacaataagc
     6181 acagggataa gcccatcagg gagcaggcag aaaacattat ccacttgttt actctgacca
     6241 acttgggcgc gcctgcagcc ttcaagtact tcgacaccac catagacaga aagcggtaca
     6301 cctctacaaa ggaggtcctg gacgccacac tgattcatca gtcaattacg gggctctatg
     6361 aaacaagaat cgacctctct cagctcggtg gggacggtgg tagtggaggt tcaggaggat
     6421 ccggggggag cggagggagc gctagcgtga ccggctaccg gctgttcgag gagattctgg
     6481 gtggaggctc cggaggtgga tctaaaaggc cggcggccac gaaaaaggcc ggtcaggcaa
     6541 aaaagaaaaa gggtggtagt ggaagcggag cggccgcatg aaagggttcg atccctaccg
     6601 gttagtaatg agtttaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa
     6661 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
     6721 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     6781 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     6841 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg
     6901 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6961 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7021 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7081 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7141 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7201 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7261 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7321 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     7381 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7441 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7501 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7561 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7621 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7681 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     7741 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     7801 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     7861 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     7921 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     7981 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     8041 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     8101 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     8161 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     8221 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     8281 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     8341 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     8401 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     8461 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     8521 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgcga
     8581 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     8641 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8701 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     8761 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8821 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     8881 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8941 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9001 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9061 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9121 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9181 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9241 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9301 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9361 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9421 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9481 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9541 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9601 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9661 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9721 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9781 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9841 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaa
//
