LOCUS       sequence-469929-        8369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An evolved Adeno-associated viral variant, enhances gene delivery 
            and gene targeting in neural stem cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Jang JH, Koerber JT, Kim JS, Asuri P, Vazin T, Bartel M, Keung A, 
            Kwon I, Park KI, Schaffer DV
  TITLE     An evolved adeno-associated viral variant enhances gene delivery and
            gene targeting in neural stem cells.
  JOURNAL   Mol Ther. 2011 Apr;19(4):667-75. doi: 10.1038/mt.2010.287. Epub 2011
            Jan 11.
  PUBMED    21224831
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2010.287"; journalName: "Mol Ther"; date: "2011-04"; 
            volume: "19"; issue: "4"; pages: "667-75"
COMMENT     Sequence Label: AAV r3.45
FEATURES             Location/Qualifiers
     source          1..8369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   832..934
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     CDS             5037..5048
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     rep_origin      5974..6487
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6061..6080)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6271..6292
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6969..6988)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7088..7110
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7148..7166)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7234..7338
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7339..7407,7408..8199)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7557..7576)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagaattc ccatcatcaa
      841 taatatacct tattttggat tgaagccaat atgataatga gggggtggag tttgtgacgt
      901 ggcgcggggc gtgggaacgg ggcgggtgac gtagtagctc tagaggtcct gtattagagg
      961 tcacgtgagt gttttgcgac attttgcgac accatgtggt cacgctgggt atttaagccc
     1021 gagtgagcac gcagggtctc cattttgaag cgggaggttt gaacgcgcag ccaccacggc
     1081 ggggttttac gagattgtga ttaaggtccc cagcgacctt gacgagcatc tgcccggcat
     1141 ttctgacagc tttgtgaact gggtggccga gaaggaatgg gagttgccgc cagattctga
     1201 catggatctg aatctgattg agcaggcacc cctgaccgtg gccgagaagc tgcagcgcga
     1261 ctttctgacg gaatggcgcc gtgtgagtaa ggccccggag gcccttttct ttgtgcaatt
     1321 tgagaaggga gagagctact tccacatgca cgtgctcgtg gaaaccaccg gggtgaaatc
     1381 catggttttg ggacgtttcc tgagtcagat tcgcgaaaaa ctgattcaga gaatttaccg
     1441 cgggatcgag ccgactttgc caaactggtt cgcggtcaca aagaccagaa atggcgccgg
     1501 aggcgggaac aaggtggtgg atgagtgcta catccccaat tacttgctcc ccaaaaccca
     1561 gcctgagctc cagtgggcgt ggactaatat ggaacagtat ttaagcgcct gtttgaatct
     1621 cacggagcgt aaacggttgg tggcgcagca tctgacgcac gtgtcgcaga cgcaggagca
     1681 gaacaaagag aatcagaatc ccaattctga tgcgccggtg atcagatcaa aaacttcagc
     1741 caggtacatg gagctggtcg ggtggctcgt ggacaagggg attacctcgg agaagcagtg
     1801 gatccaggag gaccaggcct catacatctc cttcaatgcg gcctccaact cgcggtccca
     1861 aatcaaggct gccttggaca atgcgggaaa gattatgagc ctgactaaaa ccgcccccga
     1921 ctacctggtg ggccagcagc ccgtggagga catttccagc aatcggattt ataaaatttt
     1981 ggaactaaac gggtacgatc cccaatatgc ggcttccgtc tttctgggat gggccacgaa
     2041 aaagttcggc aagaggaaca ccatctggct gtttgggcct gcaactaccg ggaagaccaa
     2101 catcgcggag gccatagccc acactgtgcc cttctacggg tgcgtaaact ggaccaatga
     2161 gaactttccc ttcaacgact gtgtcgacaa gatggtgatc tggtgggagg aggggaagat
     2221 gaccgccaag gtcgtggagt cggccaaagc cattctcgga ggaagcaagg tgcgcgtgga
     2281 ccagaaatgc aagtcctcgg cccagataga cccgactccc gtgatcgtca cctccaacac
     2341 caacatgtgc gccgtgattg acgggaactc aacgaccttc gaacaccagc agccgttgca
     2401 agaccggatg ttcaaatttg aactcacccg ccgtctggat catgactttg ggaaggtcac
     2461 caagcaggaa gtcaaagact ttttccggtg ggcaaaggat cacgtggttg aggtggagca
     2521 tgaattctac gtcaaaaagg gtggagccaa gaaaagaccc gcccccagtg acgcagatat
     2581 aagtgagccc aaacgggtgc gcgagtcagt tgcgcagcca tcgacgtcag acgcggaagc
     2641 ttcgatcaac tacgcagaca ggtaccaaaa caaatgttct cgtcacgtgg gcatgaatct
     2701 gatgctgttt ccctgcagac aatgcgagag aatgaatcag aattcaaata tctgcttcac
     2761 tcacggacag aaagactgtt tagagtgctt tcccgtgtca gaatctcaac ccgtttctgt
     2821 cgtcaaaaag gcgtatcaga aactgtgcta cattcatcat atcatgggaa aggtgccaga
     2881 cgcttgcact gcctgcgatc tggtcaatgt ggatttggat gactgcatct ttgaacaata
     2941 aatgatttaa atcaggtatg gctgccgatg gttatcttcc agattggctc gaggacactc
     3001 tctctgaagg aataagacag tggtggaagc tcaaacctgg cccaccacca ccaaagcccg
     3061 cagagcggca taaggacgac agcaggggtc ttgtgcttcc tgggtacaag tacctcggac
     3121 ccttcaacgg actcgacaag ggagagccgg tcaacgaggc agacgccgcg gccctcgagc
     3181 acgacaaagc ctacgaccgg cagctcgaca gcggagacaa cccgtacctc aagtacaacc
     3241 acgccgacgc ggagtttcag gagcgcctta aagaagatac gtcttttggg ggcaacctcg
     3301 gacgagcagt cttccaggcg aaaaagaggg ttcttgaacc tctgggcctg gttgaggaac
     3361 ctgttaagac ggctccggga aaaaagaggc cggtagagca ctctcctgtg gagccagact
     3421 cctcctcggg aaccggaaag gcgggccagc agcctgcaag aaaaagattg aattttggtc
     3481 agactggaga cgcagactca gtacctgacc cccagcctct cggacagcca ccagcagccc
     3541 cctctggtct gggaactaat acgatggcta caggcagtgg cgcaccaatg gcagacaata
     3601 acgagggcgc cgacggagtg ggtaattcct cgggaaattg gcattgcgat tccacatgga
     3661 tgggcgacag agtcatcacc accagcaccc gaacctgggc cctgcccacc tacaacaacc
     3721 acctctacaa acaaatttcc agccaatcag gagcctcgaa cgacaatcac tactttggct
     3781 acagcacccc ttgggggtat tttgacttca acagattcca ctgccacttt tcaccacgtg
     3841 actggcaaag actcatcaac aacaactggg gattccgacc caagagactc aacttcaagc
     3901 tctttaacat tcaagtcaaa gaggtcacgc agaatgacgg tacgacgacg attgccaata
     3961 accttaccag cacggttcag gtgtttactg actcggagta ccagctcccg tacgtcctcg
     4021 gctcggcgca tcaaggatgc ctcccgccgt tcccagcaga cgtcttcatg gtgccacagt
     4081 atggatacct caccctgaac aacgggagtc aggcagtagg acgctcttca ttttactgcc
     4141 tggagtactt tccttctcag atgctgcgta ccggaaacaa ctttaccttc agctacactt
     4201 ttgaggacgt tcctttccac agcagctacg ctcacagcca gagtctggac cgtctcatga
     4261 atcctctcat cgaccagtac ctgtattact tgagcagaac aaacactcca agtggaacca
     4321 ccacgcagtc aaggcttcag ttttctcagg ccggagcgag tgacattcgg gaccagtcta
     4381 ggaactggct tcctggaccc tgttaccgcc agcagcgagt atcaaagaca tctgcggata
     4441 acaacaacag tgaatactcg tggactggag ctaccaagta ccacctcaat ggcagagact
     4501 ctctggtgaa tccgggcccg gccatggcaa gccacaagga cgatgaagaa aagttttttc
     4561 ctcagagcgg ggttctcatc tttgggaagc aaggctcaga gaaaacaaat gtggacattg
     4621 aaaaggtcat gattacagac gaagaggaaa tcaggacaac caatcccgtg gctacggagc
     4681 agtatggttc tgtatctacc aacctccaga gaggcaacct agcaacccaa gtaggacaaa
     4741 aaactgctag gcaagcagct accgcagatg tcaacacaca aggcgttctt ccaggcatgg
     4801 tctggcagga cagagatgtg taccttcagg ggcccatctg ggcaaagatt ccacacacgg
     4861 acggacattt tcacccctct cccctcatgg gtggattcgg acttaaacac cctcctcccc
     4921 agattctcat caagaacacc ccggtacctg cgaatccttc gaccaccttc agtgcggcaa
     4981 agtttgcttc cttcatcaca cagtactcca cgggacaggt cagcgtggag atcgagtggg
     5041 agctgcagaa ggaaaacagc aaacgctgga atcccgaaat tcagtacact tccaactaca
     5101 acaagtctgt taatgtggac tttactgtgg acactaatgg catgtattca gagcctcgcc
     5161 ccattggcac cagatacctg actcgtaatc tgtaattgcg gccgcttgtt aatcaataaa
     5221 ccgtttaatt cgtttcagtt gaactttggt ctctgcgtat ttctttctta tctagtttcc
     5281 atgctctaga ggtcctgtat tagaggtcac gtgagtgttt tgcgacattt tgcgacacca
     5341 tgtggtcacg ctgggtattt aagcccgagt gagcacgcag ggtctccatt ttgaagcggg
     5401 aggtttgaac gcgcagccac cacggcgggg ttttacgaga ttgtgattaa ggtccccagc
     5461 gaccttgacg agcatctgcc cggcatttct gacagctttg tgaactgggt ggccgagaag
     5521 gaatgggagt tgccgccaga ttctgacatg gatctgaatc tgattgagca ggcacccctg
     5581 accgtggccg agaagctgca tcgctggcgt aatagcgaag aggcccgcac cgatcgccct
     5641 tcccaacagt tgcgcagcct gaatggcgaa tggcgattcc gttgcaatgg ctggcggtaa
     5701 tattgttctg gatattacca gcaaggccga tagtttgagt tcttctactc aggcaagtga
     5761 tgttattact aatcaaagaa gtattgcgac aacggttaat ttgcgtgatg gacagactct
     5821 tttactcggt ggcctcactg attataaaaa cacttctcag gattctggcg taccgttcct
     5881 gtctaaaatc cctttaatcg gcctcctgtt tagctcccgc tctgattcta acgaggaaag
     5941 cacgttatac gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     6001 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6061 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6121 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6181 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6241 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6301 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcatatt
     6361 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     6421 ttacaattta aatatttgct tatacaatct tcctgttttt ggggcttttc tgattatcaa
     6481 ccggggtaca tatgattgac atgctagttt tacgattacc gttcatcgat tctcttgttt
     6541 gctccagact ctcaggcaat gacctgatag cctttgtaga gacctctcaa aaatagctac
     6601 cctctccggc atgaatttat cagctagaac ggttgaatat catattgatg gtgatttgac
     6661 tgtctccggc ctttctcacc cgtttgaatc tttacctaca cattactcag gcattgcatt
     6721 taaaatatat gagggttcta aaaattttta tccttgcgtt gaaataaagg cttctcccgc
     6781 aaaagtatta cagggtcata atgtttttgg tacaaccgat ttagctttat gctctgaggc
     6841 tttattgctt aattttgcta attctttgcc ttgcctgtat gatttattgg atgttggaat
     6901 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     6961 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     7021 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     7081 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     7141 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     7201 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7261 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7321 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7381 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7441 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7501 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7561 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7621 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7681 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7741 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7801 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7861 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7921 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7981 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8041 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8101 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     8161 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8221 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8281 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8341 gaccccgtag aaaagatcaa aggatcttc
//
