LOCUS       sequence-469936-       11233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MCU-MCUB fusion in abab pattern. Only the initial MCU has a 
            mitochondrial targeting sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Rai NK, Eberhardt DR, Balynas AM, MacEwen MJS, Bratt AR, Sancak Y, 
            Chaudhuri D
  TITLE     Mechanism of MCUB-Dependent Inhibition of Mitochondrial Calcium 
            Uptake.
  JOURNAL   J Cell Physiol. 2025 Apr;240(4):e70033. doi: 10.1002/jcp.70033.
  PUBMED    40227803
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Physiol."; date: "2025-04"; volume: "240(4):e70033. doi"; pages: " 
            10.1002/jcp.70033"
COMMENT     Sequence Label: MCUabab-Flag-pLYS1
FEATURES             Location/Qualifiers
     source          1..11233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2890..2910
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3199..3402
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3353..3373
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             complement(5807..5818)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7310..7333
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7384..7501
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7550..8060
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7979..7997
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8082..8681
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8082..8101)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8578..8598
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8809..9042
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9114..9235
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9151..9170)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9205..9224
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9275..9410
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9430..9449)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9431..9449)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9459..9476)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9459..9475)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9468..9490)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9617..10072
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9704..9723)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9914..9935
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10098..10202
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10203..10271,10272..11063)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10421..10440)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgcc
     2881 cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2941 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     3001 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     3061 gactatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3121 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3181 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3241 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3301 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3361 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     3421 atccgctagc atggcggccg ccgcaggtag atcgctcctg ctgctcctct cctctcgggg
     3481 cggcggcggc gggggcgccg gcggctgcgg ggcgctgact gccggctgct tccctgggct
     3541 gggcgtcagc cgccaccggc agcagcagca ccaccggacg gtacaccaga ggatcgcttc
     3601 ctggcagaat ttgggagctg tttattgcag cactgttgtg ccctctgatg atgttacagt
     3661 ggtttatcaa aatgggttac ctgtgatatc tgtgaggcta ccatcccggc gtgaacgctg
     3721 tcagttcaca ctcaagccta tctctgactc tgttggtgta tttttacgac aactgcaaga
     3781 agaggatcgg ggaattgaca gagttgctat ctattcacca gatggtgttc gcgttgctgc
     3841 ttcaacagga atagacctcc tcctccttga tgactttaag ctggtcatta atgacttaac
     3901 ataccacgta cgaccaccaa aaagagacct cttaagtcat gaaaatgcag caacgctgaa
     3961 tgatgtaaag acattggtcc agcaactata caccacactg tgcattgagc agcaccagtt
     4021 aaacaaggaa agggagctta ttgaaagact agaggatctc aaagagcagc tggctcccct
     4081 ggaaaaggta cgaattgaga ttagcagaaa agctgagaag aggaccactt tggtgctatg
     4141 gggtggcctt gcctacatgg ccacacagtt tggcattttg gcccggctta cctggtggga
     4201 atattcctgg gacatcatgg agccagtaac atacttcatc acttatggaa gtgccatggc
     4261 aatgtatgca tattttgtaa tgacacgcca ggaatatgtt tatccagaag ccagagacag
     4321 acaatactta ctatttttcc ataaaggagc caaaaagtca cgttttgacc tagagaaata
     4381 caatcaactc aaggatgcaa ttgctcaggc agaaatggac cttaagagac tgagagaccc
     4441 attacaagta catctgcctc tccgacaaat tggtgaaaaa gattctagtc tcgagctgcc
     4501 tacagctaat ggcgacggcg gagcactggc tctggattct ctgacagttc ttggcgctgg
     4561 cggcaacaac gccaacgatg tcgacactgt gatatacaga cacggtcttc ctcttgtgac
     4621 gttgactctc ccttcccgaa aggagcgctg tcagttcgtc gtaaagccta tgctgtctac
     4681 tgtcggaagt ttcttgcaag atttgcagaa tgaagataag gggatcaaaa cggcagcaat
     4741 tttcacggca gacggcaaca tgatctccgc atccaccttg atggacatct tgcttatgaa
     4801 tgactttaaa cttgttatca ataaaattgc ttacgatgtc caatgcccaa aacgcgagaa
     4861 gccatctaac gaacatacgg ctgaaatgga acacatgaaa agcctcgttc accgcttgtt
     4921 tactatcctt catctggagg aatcacagaa aaaacgagaa catcatctcc tggaaaagat
     4981 agaccatctc aaagagcaac ttcagccttt ggagcaagtg aaagcaggaa tagaggcgca
     5041 cagcgaagcg aaaacaagcg gcctcctgtg ggctggcttg gcattgttga gtatccaagg
     5101 gggggctctg gcttggttga cttggtgggt ttattcatgg gacataatgg agccagtcac
     5161 ctactttatc acattcgcta actctatggt attctttgcg tactttatag ttacaaggca
     5221 agattacaca tattccgcag taaagagtag gcagttcctt cagttctttc ataaaaagag
     5281 taagcagcag cacttcgatg tacaacagta taataaactc aaagaagacc tcgcgaaggc
     5341 taaagagtct ttgaagcagg cgcggcactc actttgtctt caaatgcagg ttgaggagct
     5401 caatgaaaaa aacggatccc ttcccaccgc aaatggtgat gggggagcct tggctctcga
     5461 ttccctgact gttcttggtg cgggcgggaa taatgcgaat gatggtaccg tgacagttgt
     5521 gtaccagaat ggcttgcctg tcatttcagt gcgattgcca agtcgcagag agcgatgcca
     5581 gttcactctg aagcctatca gtgactccgt cggtgtgttt ctccggcagc tgcaagaaga
     5641 ggaccggggg atcgaccggg tcgcaattta tagccctgat ggagttcgcg tagctgcgtc
     5701 tactggcatt gacctcttgt tgctggacga tttcaagctg gtaatcaatg atctgaccta
     5761 tcatgtccgc cctcctaaga gggatttgct ttcccatgaa aacgcggcga ccctcaatga
     5821 cgtaaagacc cttgttcaac agctttacac gacgttgtgc atcgaacagc accaacttaa
     5881 taaggagcgg gaactcattg agagactgga ggatttgaag gaacaacttg ctccgttgga
     5941 aaaggtccgg atagaaattt cacgaaaggc agagaagcgg acgacgttgg tcctctgggg
     6001 aggactggca tatatggcca cacagttcgg tatactcgct aggttgacct ggtgggaata
     6061 ttcatgggat attatggagc ccgttacgta cttcataaca tacggcagcg caatggcaat
     6121 gtacgcttat tttgtaatga cgaggcagga atacgtatat ccggaagcgc gggaccgaca
     6181 atacttgctc ttcttccaca agggtgcgaa aaagagtagg tttgacctcg aaaagtataa
     6241 tcagcttaag gatgccattg cccaagcgga gatggacctt aaacgcttga gagatccctt
     6301 gcaagtacac ttgcctctcc ggcaaattgg agagaaggat accggtctgc caactgccaa
     6361 tggagatggc ggtgccctgg ccctggacag cctcaccgtg ctcggagccg gaggcaataa
     6421 cgctaatgat ctgcagacag taatttaccg gcatggcctg cccttggtga cactcaccct
     6481 cccgagccgc aaagaacgtt gtcagtttgt ggtaaaaccg atgctgagca cagtcggcag
     6541 ctttctccaa gacctgcaaa atgaagacaa gggcataaaa actgccgcga tcttcacggc
     6601 tgatggaaat atgataagtg cgtcgacttt gatggatatt ctcctgatga acgattttaa
     6661 attggttata aacaagatag catacgacgt ccaatgtcct aaaagggaga agccaagtaa
     6721 cgagcacact gccgaaatgg agcatatgaa atccctcgtc cacaggttgt ttaccatact
     6781 ccacctggag gaatcgcaga aaaaaaggga gcaccatttg ttggaaaaga tagaccatct
     6841 gaaagagcaa ctccaacctc tggagcaggt taaggccggt atagaggcac acagcgaggc
     6901 gaagacaagt ggcttgctct gggccggttt ggcgctgttg tcgatccagg gaggcgcgtt
     6961 ggcatggttg acgtggtggg tttatagctg ggacattatg gagcccgtga cctactttat
     7021 cacatttgca aatagtatgg ttttctttgc ctacttcatt gtaacacgtc aggattacac
     7081 ctacagtgca gtaaaaagta ggcagtttct gcaattcttt cataagaagt ccaagcagca
     7141 gcattttgac gtgcagcaat acaacaagct caaagaagat ttggcaaaag ccaaagaaag
     7201 cctcaagcag gcgcgtcact cgctgtgtct ccaaatgcag gtagaggaac tgaatgaaaa
     7261 gaattctaga ggtggatctg gtggatctgg tggatctggt ggatctatgg attacaagga
     7321 tgacgatgac aagtaaggta ccgaattctc gacctcgaga caaatggcag tattcatcca
     7381 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     7441 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     7501 tcgggtttat tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt
     7561 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     7621 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     7681 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     7741 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     7801 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     7861 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     7921 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     7981 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     8041 tcaccgacct ctctccccag ggggatccac cggagcttac catgaccgag tacaagccca
     8101 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     8161 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     8221 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     8281 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     8341 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     8401 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     8461 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     8521 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     8581 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     8641 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     8701 agcgcccgac cgaaaggagc gcacgacccc atgcatcggt acctttaaga ccaatgactt
     8761 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8821 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8881 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8941 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9001 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     9061 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     9121 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9181 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9241 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9301 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9361 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     9421 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     9481 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     9541 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     9601 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9661 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9721 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9781 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9841 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9901 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9961 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    10021 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    10081 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    10141 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    10201 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    10261 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    10321 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    10381 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    10441 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    10501 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    10561 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    10621 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10681 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10741 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10801 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10861 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10921 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10981 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    11041 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    11101 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    11161 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    11221 tcaaaggatc ttc
//
