LOCUS       sequence-469941-        9095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MCUB tagged with mVenus for FRET / MW change for stoichiometry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Rai NK, Eberhardt DR, Balynas AM, MacEwen MJS, Bratt AR, Sancak Y, 
            Chaudhuri D
  TITLE     Mechanism of MCUB-Dependent Inhibition of Mitochondrial Calcium 
            Uptake.
  JOURNAL   J Cell Physiol. 2025 Apr;240(4):e70033. doi: 10.1002/jcp.70033.
  PUBMED    40227803
REFERENCE   2  (bases 1 to 9095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Physiol."; date: "2025-04"; volume: "240(4):e70033. doi"; pages: " 
            10.1002/jcp.70033"
COMMENT     Sequence Label: MCUb-mVenus-pLJM
FEATURES             Location/Qualifiers
     source          1..9095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1417..2130
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(1456..1477)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1717..1736)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2064..2085
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2178..2295
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2344..2854
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2773..2791
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2876..3475
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2876..2895)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3372..3392
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3603..3836
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3908..4029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3945..3964)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3999..4018
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4069..4204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4224..4243)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4225..4243)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4253..4270)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4253..4269)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4262..4284)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4411..4866
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4498..4517)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4708..4729
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4892..4996
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4997..5065,5066..5857)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5215..5234)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6028..6616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6517..6536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6770..6787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6904..6925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6940..6945,6946..6963,6964..6970)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6978..6994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6983..7005
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7002..7018
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7002..7018
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7037..7057
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7039..7057
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7085..7311
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7312..7492
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7539..7664
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8157..8390
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8575..8619
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8917..8937
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccggct
      361 agcatgctcc agaggggcct ctggccgtgg cgcacgcggc tgctgccgac ccctggcacc
      421 tggcgcccag cgcgcccgtg gccgctgccg cctccgcccc aggttttgcg tgtgaagctg
      481 tgtggaaatg tgaaatacta ccagtcacac cattatagta ccgtggtgcc acctgatgaa
      541 ataacagtta attatagaca tggccttccc ttggtaacac ttaccttgcc atctagaaaa
      601 gaacgttgtc aattcgtagt caaaccaatg ttgtcaacag ttggttcatt ccttcaggac
      661 ctacaaaatg aagataaggg tatcaaaact gcagccatct tcacagcaga tggcaacatg
      721 atttcagctt ctaccttgat ggatattttg ctaatgaatg attttaaact tgtcattaat
      781 aaaatagcat atgatgtgca gtgtccaaag agagaaaaac caagtaatga gcacactgct
      841 gagatggaac acatgaaatc tttggttcac agactattta caatcttgca tttagaagag
      901 tctcagaaaa agagagagca ccatttactg gagaaaattg accacctgaa ggaacagctg
      961 cagccccttg aacaggtgaa agctggaata gaagctcatt cggaagccaa aaccagtgga
     1021 ctcctgtggg ctggattggc actgctgtcc attcagggtg gggcactggc ctggctcacg
     1081 tggtgggtgt actcctggga tatcatggag ccagttacat acttcatcac atttgcaaat
     1141 tctatggtct tttttgcata ctttatagtc actcgacagg attatactta ctcagctgtt
     1201 aagagtaggc aatttcttca gttcttccac aagaaatcaa agcaacagca ctttgatgtg
     1261 cagcaataca acaagttaaa agaagacctt gctaaggcta aagaatccct gaaacaggcg
     1321 cgtcattctc tctgtttgca aatgcaagta gaagaactca atgaaaagaa ttctttaaac
     1381 attggtggga tatcaaattc tgcagtcgac ggtaccagca agggcgagga gctgttcacc
     1441 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1501 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct catctgcacc
     1561 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctcggcta cggcctgcag
     1621 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1681 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1741 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1801 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1861 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     1921 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1981 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa
     2041 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2101 actctcggca tggacgagct gtacaagtaa ggtaccgaat tctcgacctc gagacaaatg
     2161 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2221 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2281 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc
     2341 gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
     2401 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
     2461 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
     2521 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
     2581 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
     2641 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
     2701 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
     2761 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
     2821 cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac
     2881 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     2941 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     3001 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     3061 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     3121 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     3181 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     3241 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     3301 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     3361 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     3421 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     3481 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt
     3541 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     3601 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     3661 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     3721 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     3781 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     3841 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     3901 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3961 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4021 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     4081 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4141 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4201 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     4261 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4321 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4381 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     4441 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4501 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4561 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4621 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4681 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4741 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4801 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4861 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4921 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4981 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5041 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5101 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5161 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5221 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5281 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5341 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5401 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5461 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5521 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5581 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5641 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5701 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5761 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5821 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5881 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5941 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6001 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6061 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6121 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6181 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6241 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6301 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6361 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6421 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6481 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6541 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6601 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6661 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6721 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6781 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6841 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6901 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     6961 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     7021 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     7081 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     7141 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     7201 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     7261 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     7321 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7381 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7441 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     7501 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     7561 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     7621 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     7681 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     7741 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     7801 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     7861 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     7921 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     7981 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     8041 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     8101 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     8161 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     8221 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     8281 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     8341 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     8401 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     8461 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     8521 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     8581 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     8641 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     8701 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     8761 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     8821 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
     8881 tcacgagact agcctcgagc ggccgccccc ttcaccgagg gcctatttcc catgattcct
     8941 tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta
     9001 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     9061 gcagttttaa aattatgttt taaaatggac tatca
//
