LOCUS       sequence-469942-       11230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MCU-MCUB fusion in abbb pattern. Only the initial MCU has a 
            mitochondrial targeting sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Rai NK, Eberhardt DR, Balynas AM, MacEwen MJS, Bratt AR, Sancak Y, 
            Chaudhuri D
  TITLE     Mechanism of MCUB-Dependent Inhibition of Mitochondrial Calcium 
            Uptake.
  JOURNAL   J Cell Physiol. 2025 Apr;240(4):e70033. doi: 10.1002/jcp.70033.
  PUBMED    40227803
REFERENCE   2  (bases 1 to 11230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Physiol."; date: "2025-04"; volume: "240(4):e70033. doi"; pages: " 
            10.1002/jcp.70033"
COMMENT     Sequence Label: MCUabbb-Flag-PLYS1
FEATURES             Location/Qualifiers
     source          1..11230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2890..2910
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3199..3402
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3353..3373
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             7307..7330
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7381..7498
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7547..8057
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7976..7994
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8079..8678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8079..8098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8575..8595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8806..9039
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9111..9232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9148..9167)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9202..9221
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9272..9407
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9427..9446)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9428..9446)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9456..9473)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9456..9472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9465..9487)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9614..10069
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9701..9720)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9911..9932
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10095..10199
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10200..10268,10269..11060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10418..10437)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgcc
     2881 cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2941 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     3001 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     3061 gactatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3121 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3181 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3241 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3301 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3361 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     3421 atccgctagc atggcggccg ccgcaggtag atcgctcctg ctgctcctct cctctcgggg
     3481 cggcggcggc gggggcgccg gcggctgcgg ggcgctgact gccggctgct tccctgggct
     3541 gggcgtcagc cgccaccggc agcagcagca ccaccggacg gtacaccaga ggatcgcttc
     3601 ctggcagaat ttgggagctg tttattgcag cactgttgtg ccctctgatg atgttacagt
     3661 ggtttatcaa aatgggttac ctgtgatatc tgtgaggcta ccatcccggc gtgaacgctg
     3721 tcagttcaca ctcaagccta tctctgactc tgttggtgta tttttacgac aactgcaaga
     3781 agaggatcgg ggaattgaca gagttgctat ctattcacca gatggtgttc gcgttgctgc
     3841 ttcaacagga atagacctcc tcctccttga tgactttaag ctggtcatta atgacttaac
     3901 ataccacgta cgaccaccaa aaagagacct cttaagtcat gaaaatgcag caacgctgaa
     3961 tgatgtaaag acattggtcc agcaactata caccacactg tgcattgagc agcaccagtt
     4021 aaacaaggaa agggagctta ttgaaagact agaggatctc aaagagcagc tggctcccct
     4081 ggaaaaggta cgaattgaga ttagcagaaa agctgagaag aggaccactt tggtgctatg
     4141 gggtggcctt gcctacatgg ccacacagtt tggcattttg gcccggctta cctggtggga
     4201 atattcctgg gacatcatgg agccagtaac atacttcatc acttatggaa gtgccatggc
     4261 aatgtatgca tattttgtaa tgacacgcca ggaatatgtt tatccagaag ccagagacag
     4321 acaatactta ctatttttcc ataaaggagc caaaaagtca cgttttgacc tagagaaata
     4381 caatcaactc aaggatgcaa ttgctcaggc agaaatggac cttaagagac tgagagaccc
     4441 attacaagta catctgcctc tccgacaaat tggtgaaaaa gattctagtc tcgagctgcc
     4501 tacagctaat ggcgacggcg gagcactggc tctggattct ctgacagttc ttggcgctgg
     4561 cggcaacaac gccaacgatg tcgacactgt gatatacaga cacggtcttc ctcttgtgac
     4621 gttgactctc ccttcccgaa aggagcgctg tcagttcgtc gtaaagccta tgctgtctac
     4681 tgtcggaagt ttcttgcaag atttgcagaa tgaagataag gggatcaaaa cggcagcaat
     4741 tttcacggca gacggcaaca tgatctccgc atccaccttg atggacatct tgcttatgaa
     4801 tgactttaaa cttgttatca ataaaattgc ttacgatgtc caatgcccaa aacgcgagaa
     4861 gccatctaac gaacatacgg ctgaaatgga acacatgaaa agcctcgttc accgcttgtt
     4921 tactatcctt catctggagg aatcacagaa aaaacgagaa catcatctcc tggaaaagat
     4981 agaccatctc aaagagcaac ttcagccttt ggagcaagtg aaagcaggaa tagaggcgca
     5041 cagcgaagcg aaaacaagcg gcctcctgtg ggctggcttg gcattgttga gtatccaagg
     5101 gggggctctg gcttggttga cttggtgggt ttattcatgg gacataatgg agccagtcac
     5161 ctactttatc acattcgcta actctatggt attctttgcg tactttatag ttacaaggca
     5221 agattacaca tattccgcag taaagagtag gcagttcctt cagttctttc ataaaaagag
     5281 taagcagcag cacttcgatg tacaacagta taataaactc aaagaagacc tcgcgaaggc
     5341 taaagagtct ttgaagcagg cgcggcactc actttgtctt caaatgcagg ttgaggagct
     5401 caatgaaaaa aacggatccc ttcccaccgc aaatggtgat gggggagcct tggctctcga
     5461 ttccctgact gttcttggtg cgggcgggaa taatgcgaat gatggtacca ccgtgatcta
     5521 cagacacggc ctgcctctgg tcaccctgac actgcctagc cggaaagaac ggtgccagtt
     5581 cgtggtcaag cccatgctgt ctaccgtggg cagcttcctc caggacctcc agaatgagga
     5641 caagggcatc aagaccgccg ccatctttac cgccgacggc aacatgatca gcgccagcac
     5701 actgatggac atcctgctga tgaacgactt caagctggtc atcaacaaga tcgcctacga
     5761 cgtgcagtgc cccaagagag agaagcccag caacgagcac accgccgaga tggaacacat
     5821 gaagtccctg gtgcaccggc tgttcaccat cctgcacctg gaagagtccc agaagaagag
     5881 agagcaccat ctgctggaaa agatcgacca cctgaaagag cagctccagc ctctggaaca
     5941 agtgaaggcc ggaatcgagg cccacagcga ggccaaaaca tctggactgc tgtgggctgg
     6001 actggccctg ctgagtattc aaggcggagc actggcttgg ctgacttggt gggtgtacag
     6061 ctgggacatc atggaacccg tgacctactt catcaccttc gccaacagca tggtgttctt
     6121 cgcctacttt atcgtgaccc ggcaggacta cacctacagc gccgtgaagt ccagacagtt
     6181 cctccagttc ttccacaaga agtccaagca gcagcacttc gatgtgcagc agtacaacaa
     6241 gctgaaagag gacctggcca aggccaaaga gagcctgaaa caggccagac acagcctgtg
     6301 cctccagatg caggtcgagg aactgaacga gaagaacacc ggtctgccaa ctgccaatgg
     6361 tgatggggga gccttggccc tggacagcct caccgtgctc ggagccggag gcaataacgc
     6421 taatgatctg cagacagtaa tttaccggca tggcctgccc ttggtgacac tcaccctccc
     6481 gagccgcaaa gaacgttgtc agtttgtggt aaaaccgatg ctgagcacag tcggcagctt
     6541 tctccaagac ctgcaaaatg aagacaaggg cataaaaact gccgcgatct tcacggctga
     6601 tggaaatatg ataagtgcgt cgactttgat ggatattctc ctgatgaacg attttaaatt
     6661 ggttataaac aagatagcat acgacgtcca atgtcctaaa agggagaagc caagtaacga
     6721 gcacactgcc gaaatggagc atatgaaatc cctcgtccac aggttgttta ccatactcca
     6781 cctggaggaa tcgcagaaaa aaagggagca ccatttgttg gaaaagatag accatctgaa
     6841 agagcaactc caacctctgg agcaggttaa ggccggtata gaggcacaca gcgaggcgaa
     6901 gacaagtggc ttgctctggg ccggtttggc gctgttgtcg atccagggag gcgcgttggc
     6961 atggttgacg tggtgggttt atagctggga cattatggag cccgtgacct actttatcac
     7021 atttgcaaat agtatggttt tctttgccta cttcattgta acacgtcagg attacaccta
     7081 cagtgcagta aaaagtaggc agtttctgca attctttcat aagaagtcca agcagcagca
     7141 ttttgacgtg cagcaataca acaagctcaa agaagatttg gcaaaagcca aagaaagcct
     7201 caagcaggcg cgtcactcgc tgtgtctcca aatgcaggta gaggaactga atgaaaagaa
     7261 ttctagaggt ggatctggtg gatctggtgg atctggtgga tctatggatt acaaggatga
     7321 cgatgacaag taaggtaccg aattctcgac ctcgagacaa atggcagtat tcatccacaa
     7381 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     7441 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     7501 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
     7561 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     7621 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     7681 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     7741 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     7801 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     7861 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     7921 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     7981 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     8041 ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac aagcccacgg
     8101 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     8161 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     8221 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     8281 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     8341 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     8401 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     8461 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     8521 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     8581 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     8641 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     8701 gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca atgacttaca
     8761 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8821 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8881 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8941 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9001 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     9061 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     9121 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9181 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9241 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9301 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9361 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     9421 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     9481 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9541 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9601 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9661 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9721 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9781 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9841 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9901 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9961 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    10021 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
    10081 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10141 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    10201 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    10261 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10321 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10381 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10441 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10501 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10561 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10621 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10681 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10741 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10801 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10861 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10921 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10981 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    11041 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    11101 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    11161 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11221 aaggatcttc
//
