LOCUS       sequence-469943-       11254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MCU-MCUB fusion in abaa pattern. Only the initial MCU has a 
            mitochondrial targeting sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Rai NK, Eberhardt DR, Balynas AM, MacEwen MJS, Bratt AR, Sancak Y, 
            Chaudhuri D
  TITLE     Mechanism of MCUB-Dependent Inhibition of Mitochondrial Calcium 
            Uptake.
  JOURNAL   J Cell Physiol. 2025 Apr;240(4):e70033. doi: 10.1002/jcp.70033.
  PUBMED    40227803
REFERENCE   2  (bases 1 to 11254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Physiol."; date: "2025-04"; volume: "240(4):e70033. doi"; pages: " 
            10.1002/jcp.70033"
COMMENT     Sequence Label: MCUabaa-Flag-PLYS1
FEATURES             Location/Qualifiers
     source          1..11254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2890..2910
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3199..3402
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3353..3373
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             complement(5807..5818)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7331..7354
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7405..7522
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7571..8081
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8000..8018
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8103..8702
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8103..8122)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8599..8619
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8830..9063
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9135..9256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9172..9191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9226..9245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9296..9431
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9451..9470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9452..9470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9480..9497)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9480..9496)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9489..9511)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9638..10093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9725..9744)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9935..9956
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10119..10223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10224..10292,10293..11084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10442..10461)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgcc
     2881 cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2941 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     3001 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     3061 gactatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3121 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3181 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3241 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3301 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3361 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     3421 atccgctagc atggcggccg ccgcaggtag atcgctcctg ctgctcctct cctctcgggg
     3481 cggcggcggc gggggcgccg gcggctgcgg ggcgctgact gccggctgct tccctgggct
     3541 gggcgtcagc cgccaccggc agcagcagca ccaccggacg gtacaccaga ggatcgcttc
     3601 ctggcagaat ttgggagctg tttattgcag cactgttgtg ccctctgatg atgttacagt
     3661 ggtttatcaa aatgggttac ctgtgatatc tgtgaggcta ccatcccggc gtgaacgctg
     3721 tcagttcaca ctcaagccta tctctgactc tgttggtgta tttttacgac aactgcaaga
     3781 agaggatcgg ggaattgaca gagttgctat ctattcacca gatggtgttc gcgttgctgc
     3841 ttcaacagga atagacctcc tcctccttga tgactttaag ctggtcatta atgacttaac
     3901 ataccacgta cgaccaccaa aaagagacct cttaagtcat gaaaatgcag caacgctgaa
     3961 tgatgtaaag acattggtcc agcaactata caccacactg tgcattgagc agcaccagtt
     4021 aaacaaggaa agggagctta ttgaaagact agaggatctc aaagagcagc tggctcccct
     4081 ggaaaaggta cgaattgaga ttagcagaaa agctgagaag aggaccactt tggtgctatg
     4141 gggtggcctt gcctacatgg ccacacagtt tggcattttg gcccggctta cctggtggga
     4201 atattcctgg gacatcatgg agccagtaac atacttcatc acttatggaa gtgccatggc
     4261 aatgtatgca tattttgtaa tgacacgcca ggaatatgtt tatccagaag ccagagacag
     4321 acaatactta ctatttttcc ataaaggagc caaaaagtca cgttttgacc tagagaaata
     4381 caatcaactc aaggatgcaa ttgctcaggc agaaatggac cttaagagac tgagagaccc
     4441 attacaagta catctgcctc tccgacaaat tggtgaaaaa gattctagtc tcgagctgcc
     4501 tacagctaat ggcgacggcg gagcactggc tctggattct ctgacagttc ttggcgctgg
     4561 cggcaacaac gccaacgatg tcgacactgt gatatacaga cacggtcttc ctcttgtgac
     4621 gttgactctc ccttcccgaa aggagcgctg tcagttcgtc gtaaagccta tgctgtctac
     4681 tgtcggaagt ttcttgcaag atttgcagaa tgaagataag gggatcaaaa cggcagcaat
     4741 tttcacggca gacggcaaca tgatctccgc atccaccttg atggacatct tgcttatgaa
     4801 tgactttaaa cttgttatca ataaaattgc ttacgatgtc caatgcccaa aacgcgagaa
     4861 gccatctaac gaacatacgg ctgaaatgga acacatgaaa agcctcgttc accgcttgtt
     4921 tactatcctt catctggagg aatcacagaa aaaacgagaa catcatctcc tggaaaagat
     4981 agaccatctc aaagagcaac ttcagccttt ggagcaagtg aaagcaggaa tagaggcgca
     5041 cagcgaagcg aaaacaagcg gcctcctgtg ggctggcttg gcattgttga gtatccaagg
     5101 gggggctctg gcttggttga cttggtgggt ttattcatgg gacataatgg agccagtcac
     5161 ctactttatc acattcgcta actctatggt attctttgcg tactttatag ttacaaggca
     5221 agattacaca tattccgcag taaagagtag gcagttcctt cagttctttc ataaaaagag
     5281 taagcagcag cacttcgatg tacaacagta taataaactc aaagaagacc tcgcgaaggc
     5341 taaagagtct ttgaagcagg cgcggcactc actttgtctt caaatgcagg ttgaggagct
     5401 caatgaaaaa aacggatccc ttcccaccgc aaatggtgat gggggagcct tggctctcga
     5461 ttccctgact gttcttggtg cgggcgggaa taatgcgaat gatggtaccg tgacagttgt
     5521 gtaccagaat ggcttgcctg tcatttcagt gcgattgcca agtcgcagag agcgatgcca
     5581 gttcactctg aagcctatca gtgactccgt cggtgtgttt ctccggcagc tgcaagaaga
     5641 ggaccggggg atcgaccggg tcgcaattta tagccctgat ggagttcgcg tagctgcgtc
     5701 tactggcatt gacctcttgt tgctggacga tttcaagctg gtaatcaatg atctgaccta
     5761 tcatgtccgc cctcctaaga gggatttgct ttcccatgaa aacgcggcga ccctcaatga
     5821 cgtaaagacc cttgttcaac agctttacac gacgttgtgc atcgaacagc accaacttaa
     5881 taaggagcgg gaactcattg agagactgga ggatttgaag gaacaacttg ctccgttgga
     5941 aaaggtccgg atagaaattt cacgaaaggc agagaagcgg acgacgttgg tcctctgggg
     6001 aggactggca tatatggcca cacagttcgg tatactcgct aggttgacct ggtgggaata
     6061 ttcatgggat attatggagc ccgttacgta cttcataaca tacggcagcg caatggcaat
     6121 gtacgcttat tttgtaatga cgaggcagga atacgtatat ccggaagcgc gggaccgaca
     6181 atacttgctc ttcttccaca agggtgcgaa aaagagtagg tttgacctcg aaaagtataa
     6241 tcagcttaag gatgccattg cccaagcgga gatggacctt aaacgcttga gagatccctt
     6301 gcaagtacac ttgcctctcc ggcaaattgg agagaaggat accggtctgc caactgccaa
     6361 tggagatggc ggtgccctgg ccctggacag cctcaccgtg ctcggagccg gaggcaataa
     6421 cgctaatgat ctgcaggtaa ctgtggtata tcaaaacggt ctcccagtga tctcggtgcg
     6481 gctgccgagc cgccgagagc ggtgtcagtt cacgctcaag ccaataagcg attcggtagg
     6541 tgtattcttg cgacaactcc aggaagagga caggggaatt gatcgtgtgg ctatctactc
     6601 gcccgatggt gttcgtgtgg ctgccagtac aggcattgac ctgctcctcc tcgacgattt
     6661 taaactggtg attaacgatt tgacctacca tgtacgcccc cccaaacgtg atctcctgtc
     6721 gcacgagaat gcagcaacgt tgaacgatgt caagacattg gtacaacagc tgtacacgac
     6781 cttgtgcatt gagcaacacc agctcaataa agaaagggag ctcatcgaac gcttggaaga
     6841 cttgaaggaa cagctggctc cgttggagaa ggtgaggata gaaatcagcc ggaaagccga
     6901 aaagcggacg acactcgttc tgtggggtgg cctggcatat atggcgaccc agttcggtat
     6961 tctcgcacga ctgacgtggt gggaatatag ttgggatatc atggaacctg tgacgtactt
     7021 tatcacttat ggctccgcta tggctatgta tgcatacttt gtaatgacac gtcaggagta
     7081 cgtctatcct gaagcccgag acaggcaata tctgctcttt ttccacaaag gcgccaaaaa
     7141 gtcccggttc gatctcgaaa agtacaatca gttgaaagat gctatcgcgc aagcggagat
     7201 ggacctgaaa cggctccgcg atcctctcca ggtccacctg cccttgcggc agataggtga
     7261 gaaggattct agaggtggat ctggtggatc tggtggatct ggtggatctg gtggatctgg
     7321 tggatctatg gattacaagg atgacgatga caagtaaggt accgaattct cgacctcgag
     7381 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     7441 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     7501 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
     7561 gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
     7621 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
     7681 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
     7741 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
     7801 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag
     7861 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg
     7921 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg
     7981 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag
     8041 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta
     8101 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     8161 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     8221 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     8281 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     8341 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     8401 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     8461 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     8521 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     8581 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     8641 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     8701 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg
     8761 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8821 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     8881 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8941 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     9001 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9061 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     9121 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9181 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9241 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     9301 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9361 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     9421 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     9481 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     9541 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     9601 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     9661 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     9721 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9781 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9841 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9901 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9961 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    10021 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    10081 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    10141 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    10201 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    10261 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    10321 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    10381 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    10441 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
    10501 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    10561 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    10621 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    10681 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    10741 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    10801 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    10861 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    10921 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    10981 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    11041 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    11101 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    11161 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    11221 cgtcagaccc cgtagaaaag atcaaaggat cttc
//
