LOCUS       sequence-469944-        9070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Clone MCUB into pLYS5 (Hygro resistance) with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Rai NK, Eberhardt DR, Balynas AM, MacEwen MJS, Bratt AR, Sancak Y, 
            Chaudhuri D
  TITLE     Mechanism of MCUB-Dependent Inhibition of Mitochondrial Calcium 
            Uptake.
  JOURNAL   J Cell Physiol. 2025 Apr;240(4):e70033. doi: 10.1002/jcp.70033.
  PUBMED    40227803
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Physiol."; date: "2025-04"; volume: "240(4):e70033. doi"; pages: " 
            10.1002/jcp.70033"
COMMENT     Sequence Label: MCUb-HA-pLYS5
FEATURES             Location/Qualifiers
     source          1..9070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    103..224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(114..133)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     168..187
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             296..529
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(906..1931)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(906..1931)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(1997..2016)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     misc_feature    2508..2625
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2676..2702)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3773..3976)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3802..3822)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(4265..4285)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(4583..4627)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4812..5045
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5538..5663
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5710..5890
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5891..6117)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(6145..6165)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6145..6163)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6184..6200)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6184..6200)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6197..6219)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6208..6224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6232..6238,6239..6256,6257..6262))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6277..6298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6415..6432)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6586..7174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6666..6685)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7345..8136,8137..8205))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7968..7987
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8206..8310)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8336..8791)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8473..8494)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8685..8704
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8918..8940
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8932..8949
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8933..8949
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8959..8978
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8959..8977
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      join(8998..9070,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggatagctag agccagacat gataagatac attgatgagt
      121 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      181 ctattgcttt atttgtaacc attataagct gcaataaaca agttcctctc actctctgat
      241 attcatttct ttgcaagtta taaatactga ataataagat gacatgaact actactgcta
      301 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
      361 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
      421 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
      481 caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
      541 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaagagctt
      601 ggctgcaggt cgacggatct gcacccaatc ggcaggcacg ggcggcgatc tccaatctgc
      661 gggatcagtc agatcacccg agtgcgtggg catgacaatc gtgccctggg gaccaacaca
      721 atccagaagg gcctgaatca ctgcgaccgg ccctcccgcg acccagccga gcgagcttag
      781 cgaactgtgg acgagaactg tgccaccaag cgtaaggccg ttctctcgca tttgccttgc
      841 taggctcgcg cgagttgctg gctgaggcgt tctcgaaatc agctcttgtt cggtcggcat
      901 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
      961 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     1021 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     1081 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     1141 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     1201 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     1261 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     1321 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     1381 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     1441 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     1501 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     1561 gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca
     1621 ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg
     1681 ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
     1741 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
     1801 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
     1861 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
     1921 ggctttttca tggtggcggg atgcaggtcg aaaggcccgg agatgaggaa gaggagaaca
     1981 gcgcggcaga cgtgcgcttt tgaagcgtgc agaatgccgg gcctccggag gaccttcggg
     2041 cgcccgcccc gcccctgagc ccgcccctga gcccgccccc ggacccaccc cttcccagcc
     2101 tctgagccca gaaagcgaag gagcaaagct gctattggcc gctgccccaa aggcctaccc
     2161 gcttccattg ctcagcggtg ctgtccatct gcacgagact agtgagacgt gcggcttccg
     2221 tttgtcacgt ccggcacgcc gcgaaccgca aggaaccttc ccgacttagg ggcggagcag
     2281 gaagcgtcgc cggggggccc acaagggtag cggcgaagat ccgggtgacg ctgcgaacgg
     2341 acgtgaagaa tgtgcgagac ccagggtcgg cgccgctgcg tttcccggaa ccacgcccag
     2401 agcagccgcg tccctgcgca aacccagggc tgccttggaa aaggcgcaac cccaaccccc
     2461 tcgagccgcg gccaaagtgg atctctgctg tccctgtaat aaacccgaaa attttgaatt
     2521 tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt
     2581 ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact
     2641 gccatttgtc tcgaggtcga gaattcggta ccttaagcgt aatctggaac atcgtatggg
     2701 taagatccac cagatccacc agatccacct ctagaattct tttcattgag ttcttctact
     2761 tgcatttgca aacagagaga atgacgcgcc tgtttcaggg attctttagc cttagcaagg
     2821 tcttctttta acttgttgta ttgctgcaca tcaaagtgct gttgctttga tttcttgtgg
     2881 aagaactgaa gaaattgcct actcttaaca gctgagtaag tataatcctg tcgagtgact
     2941 ataaagtatg caaaaaagac catagaattt gcaaatgtga tgaagtatgt aactggctcc
     3001 atgatatccc aggagtacac ccaccacgtg agccaggcca gtgccccacc ctgaatggac
     3061 agcagtgcca atccagccca caggagtcca ctggttttgg cttccgaatg agcttctatt
     3121 ccagctttca cctgttcaag gggctgcagc tgttccttca ggtggtcaat tttctccagt
     3181 aaatggtgct ctctcttttt ctgagactct tctaaatgca agattgtaaa tagtctgtga
     3241 accaaagatt tcatgtgttc catctcagca gtgtgctcat tacttggttt ttctctcttt
     3301 ggacactgca catcatatgc tattttatta atgacaagtt taaaatcatt cattagcaaa
     3361 atatccatca aggtagaagc tgaaatcatg ttgccatctg ctgtgaagat ggctgcagtt
     3421 ttgataccct tatcttcatt ttgtaggtcc tgaaggaatg aaccaactgt tgacaacatt
     3481 ggtttgacta cgaattgaca acgttctttt ctagatggca aggtaagtgt taccaaggga
     3541 aggccatgtc tataattaac tgttatttca tcaggtggca ccacggtact ataatggtgt
     3601 gactggtagt atttcacatt tccacacagc ttcacacgca aaacctgggg cggaggcggc
     3661 agcggccacg ggcgcgctgg gcgccaggtg ccaggggtcg gcagcagccg cgtgcgccac
     3721 ggccagaggc ccctctggag catgctagcc ggatctgacg gttcactaaa ccagctctgc
     3781 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     3841 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     3901 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     3961 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     4021 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     4081 cgtcaatagg gggcgtactt ggcatatgat agtccatttt aaaacataat tttaaaactg
     4141 caaactaccc aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt
     4201 ttacagtcaa attaattcca attatctctc taacagcctt gtatcgtata tgcaaatatg
     4261 aaggaatcat gggaaatagg ccctcggtga agggggcggc cgctcgaggc tagtctcgtg
     4321 atcgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct ctctctccac
     4381 cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa
     4441 acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta
     4501 ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat
     4561 ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt
     4621 cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat
     4681 tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt
     4741 tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac
     4801 cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct
     4861 ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc
     4921 tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca
     4981 gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag
     5041 ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg
     5101 ctactcctaa tggttcaatt tttactactt tatatttata taattcactt ctccaattgt
     5161 ccctcatatc tcctcctcca ggtctgaaga tcagcggccg cttgctgtgc ggtggtctta
     5221 cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt tatctctatc
     5281 ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat
     5341 cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct
     5401 aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt
     5461 aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt
     5521 ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc
     5581 aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca
     5641 agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc
     5701 gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     5761 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     5821 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     5881 agagagaccc gtttatgtat cgagctaggc acttaaatac aatatctctg caatgcggca
     5941 attcagtggt tcgtccaatc catgtcagac ccgtctgttg ccttcctaat aaggcacgat
     6001 cgtaccacct tacttccacc aatcggcatg cacggtgctt tttctctcct tgtaaggcat
     6061 gttgctaact catcgttacc atgttgcaag actacaagag tattgcataa gactacatta
     6121 agcttgcagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     6181 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6241 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6301 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6361 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6421 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6481 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6541 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6601 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6661 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6721 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6781 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6841 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6901 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6961 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7021 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7081 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7141 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7201 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7261 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7321 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7381 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7441 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7501 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7561 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7621 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7681 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7741 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7801 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7861 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7921 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7981 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8041 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8101 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8161 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8221 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8281 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     8341 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     8401 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     8461 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     8521 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     8581 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     8641 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     8701 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     8761 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     8821 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     8881 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     8941 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac gtccctaggc
     9001 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     9061 ctgcataaat
//
