LOCUS       sequence-469948-        9860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a fluorescent lipid droplet-localized APEX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Cunningham CN, Van Vranken JG, Larios J, Heyden K, Gygi SP, Rutter J
  TITLE     A dual-purification system to isolate mitochondrial subpopulations.
  JOURNAL   J Cell Sci. 2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693.
  PUBMED    40079232
REFERENCE   2  (bases 1 to 9860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693."
COMMENT     Sequence Label: pQCXIP-EGFP-ADRP-APEX2-V5
FEATURES             Location/Qualifiers
     source          1..9860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(882..1078)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(890..1100)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      896..1031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1124..1144
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     1280..1302
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     LTR             1405..1830
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     CDS             complement(2204..2803)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2287..2307)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2784..2803
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2824..3323)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2872..2891)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3265..3288
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(3348..3389)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(3390..4136)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             complement(join(5493..6203,6204..6206,6207..6209))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5493..6203,6204..6206,6207..6209))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5535..5556)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5884..5903
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6143..6164
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6206..6215
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6336..6360)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6357..6560)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        6561..6864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6901..7317)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(6917..6933)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6949..6971)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    7518..7717
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             7780..7955
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(7956..8159)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        8160..8539
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(8639..8657)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8725..8829
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8830..8898,8899..9690)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9048..9067)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtaagct ttttgcaaaa gcctaggcct
      901 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
      961 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     1021 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     1081 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     1141 ttccacagcc ggatctacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     1201 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     1261 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1321 aaacgcgcga ggcagccagc ttacctcccg gtggtgggtc ggtggtccct gggcaggggt
     1381 ctcccgatcc cggacgagcc cccaaatgaa agacccccgc tgacgggtag tcaatcactc
     1441 agaggagacc ctcccaagga acagcgagac cacaagtcgg atgcaactgc aagagggttt
     1501 attggataca cgggtacccg ggcgactcag tcaatcggag gactggcgcc ccgagtgagg
     1561 ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg
     1621 aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg gcaccctgga
     1681 aacatctgat ggttctctag ggtcgaccct gttccatctg ttcctgacct tgatctgaac
     1741 ttctctattc tcagttatgt atttttccat gccttgcaaa atggcgttac ttaagctagc
     1801 ttgccaaacc tacaggtggg gtctttcatt cccccctttt tctggagact aaataaaatc
     1861 ttttatttta tctatggctc gtactctata ggcttcagct ggtgatattg ttgagtcaaa
     1921 actagagcct ggaccactga tatcctgtct ttaacaaatt ggactaatcg ataagcttat
     1981 ctctacactg gctcgatgga gtcagcctag tgtccctcgg aaagccttga ggtccctttg
     2041 gagcagttct gcactcggcc tctgcagcgc tgcagccctc gactagagtc ggtgggcctc
     2101 gggggcgggt gcggggtcgg cggggccgcc ccgggtggct tcggtcggag ccatggggtc
     2161 gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc
     2221 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     2281 cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc
     2341 cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct
     2401 ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg
     2461 gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca
     2521 tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga
     2581 ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg
     2641 tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccggg tcgacggtgt
     2701 ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt
     2761 cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttgggctgca
     2821 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
     2881 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
     2941 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
     3001 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
     3061 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
     3121 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
     3181 accaaagaac ggagtcgctt ggcgcctaac gctggatgtg gaatgtgtgc gaggccagag
     3241 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
     3301 tgggaaaagc gcctccccta cccggttcac gggtttgttt aaactcaggt gctgtccagg
     3361 cccagcaggg ggttggggat gggcttgccg gcatcagcaa acccaagctc ggaaagcttt
     3421 tggtgagcct cagcgtaatc agcaaagaag gcatcttcgt ccgctgcata tttgtcaacg
     3481 agagggcgga atacagggtc agacaaaaga gccttgtcag aaggtagctg aaggagacct
     3541 tccttctcac cactcaacaa ctccgtgaag tatgagttgt cgaaaataag aggattagag
     3601 gtccagggac cctcaaatcc agaacgctcc ttgtgtgcag ctccaatagt gtgaccccca
     3661 gatagagcaa cgatatcttg gtcagtaagc cccatagctt tgccaaacac atctctcaaa
     3721 tggtcagaac ccttagtggg atcgggcaag cgaccctctg gtggtggctc aggcttgtcc
     3781 tctcttccag ggtggaatgg aaccttaggt ccacccgtga cctcaacggc aacaacgcca
     3841 gccaactggt agaaatcggc gtagctcaaa atagggaact ccgccttgag tggctccaaa
     3901 agcctaacag cgatgtcaag accgttgtta gcgctgtgag ccagttcggc agggtgcttg
     3961 atggttccga agggtccacc ggtcttcgtg cccttgtcaa aggttccagc agagtggaat
     4021 gccaaacgga gcattagagg agcgcatctc ttctcagcga tgaagcctct gagcttcttc
     4081 ttcgccttct caacggcgtc ctggtaatca gcactcacag ttgggtaaga ctttccaatc
     4141 cggagacgct cgagggggta atgagtttta tgctcagatc gctgggtctc ctggctgctc
     4201 ttgtccatct ctgcaccttg gtcctgagca ttctgagact cagtcagctg aggataaaag
     4261 ggacctacca gccagttgag gggcgtgttg ttaacaagat aatccatcac gtcatctaaa
     4321 gattccttca ttttctgcag ctgccccttg ctagaagtga ggaggctgtc agacacttct
     4381 ttaaaggagg cagcattgcg gaacactgag tagatgtcgc ctgccatcac ccccatgtgc
     4441 ttggcttgat cttggatgtt ctgtggtaca ccttggatgt tggacaggag ggtgtggcac
     4501 gtggtctgga gctgctgagt caggttgcgg gcaattgcaa gagtacgtga ctcaatgtgc
     4561 tcagcacagt gggactcatc agtatcatca tatccaatgc tccttttcca ctctacccat
     4621 gagaggtaga gcttatcctg agcatcctga attttctgat tggcactata cacattcttc
     4681 ctggcaaatt caatcaggtg aacagtagaa tggagctgag aaatggtctg ttggcttttt
     4741 tgcttagctt ctttaaccct gctgagagcc tgctggtagg cacgggagtg aagcttggta
     4801 gacagggatc ccagtctaac ataataactt ggcttctgaa ccagatcaaa tccttcaact
     4861 ttttttgctt ctttttctag ttcttcctca gtgagaggga ggtactgttc taccaacagc
     4921 tctgatttgg tgagtgcatt ttctacgcca ctgctcacga gctgcatcat ccgactcccc
     4981 aagactgtgt taatgctgcc actgaccaca gacttggtct tctccacact gccagtcact
     5041 gcccctttgg tcttgtccat cacccctgtg atcgtgctgg ccacagaatc cttggcccca
     5101 gtcacagtag tcgtcacagc atcttttgcc ccagtcacag cgcctttggc attggcaaca
     5161 atctgagttg atggctgatt cagaataggc agtctctcct caatcctgtc tagcccctta
     5221 caggcatagg tattggcaac tgcaatttgc ggctctagct tctggatgat gggcagagca
     5281 ctggtcatgg ccacggaggt gatggtcttc acaccgttct ctgccatctc acacacagac
     5341 ttcaggtagg gatactggtc ctttgtactg agataggctg aggacatgag gtcatacgtg
     5401 gagctcacca agggcaggtt gaccacccga gtcaccacac tcggttgtgg atcaactgca
     5461 acggatgcca tagctcgaga tctgagtccg gacttgtaca gctcgtccat gccgagagtg
     5521 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     5581 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     5641 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
     5701 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
     5761 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     5821 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     5881 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     5941 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
     6001 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     6061 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
     6121 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     6181 gtgaacagct cctcgccctt gctcaccatg gtggcgacgc ggccgcggat caattcctgc
     6241 aggtccgttc caatgcaccg ttcccggccg cggaggctgg atcggtcccg gtgtcttcta
     6301 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct
     6361 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
     6421 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
     6481 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
     6541 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
     6601 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
     6661 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
     6721 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
     6781 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
     6841 ccatttaccg taagttatgt aacggactct agaggatcga tcccccagat cttcaattcc
     6901 ggcgcctaga gaaggagtga gggctggata aagggaggat cgaggcgggg tcgaacgagg
     6961 aggttcaagg gggagagacg gggcggatgg aggaagagga ggcggaggct tagggtgtac
     7021 aaagggcttg acccagggag gggggtcaaa agccaaggct tcccaggtca cgatgtaggg
     7081 gacctggtct gggtgtccat gcgggccagg tgaaaagacc ttgatcttaa cctgggtgat
     7141 gaggtctcgg ttaaaggtgc cgtctcgcgg ccatccgacg ttaaaggttg gccattctgc
     7201 agagcagaag gtaacccaac gtctcttctt gacatctacc gactggttgt gagcgatccg
     7261 ctcgacatct ttccagtgac ctaaggtcaa acttaaggga gtggtaacag tctggcccta
     7321 attttcagac aaatacagaa acacagtcag acagagacaa cacagaacga tgctgcagcg
     7381 ctgcagcaga caagacgcgc ggcttcggtt ccaaaccgaa agcaaaaatt cagacggagg
     7441 cgggaactgt tttaggttct cgtctcctac cagaaccaca tatcctgacg gggtcggatt
     7501 ccacatcgac tcccttcctc aggtcgggcc acaaaaacgg cccccaaagt ccctgggacg
     7561 tctcccaggg ttgcggccgg gtgttcagaa ctcgtcagtt ccaccacggg tccgccagat
     7621 acagagctag ttagctaact agtacagacg caggcgcata acatcaaaca tagacactag
     7681 acaatcggac agacacagat aagttgctgg ccagcttacc tcccggtggt gggtcggtgg
     7741 tccctgggca ggggtctcca aatcccggac gagcccccaa atgaaagacc cccgtcgtgg
     7801 gtagtcaatc actcagagga gaccctccca aggaacagcg agaccacgat tcggatgcaa
     7861 acagcaagag gctttattgg gaatacgggt acccgggcga cgcagtctat cggaagactg
     7921 gcgcgccgag tgaggggttg tgggctcttt tattgagctc tgcttatata gacctcccac
     7981 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
     8041 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
     8101 aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc
     8161 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
     8221 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
     8281 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
     8341 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
     8401 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
     8461 acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa
     8521 ctagtcaata atcaatgtca acatggcggt aatgttggac atgagccaat ataaatgtac
     8581 atattatgat atggatacaa cgtatgcaat ggccaagcta attcttgaag acgaaagggc
     8641 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     8701 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8761 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8821 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8881 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8941 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9001 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9061 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9121 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9181 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9241 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9301 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9361 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9421 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9481 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9541 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9601 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9661 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9721 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9781 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9841 gaaaagatca aaggatcttc
//
