LOCUS       sequence-469949-        8893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a fluorescent Golgi-localized APEX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Cunningham CN, Van Vranken JG, Larios J, Heyden K, Gygi SP, Rutter J
  TITLE     A dual-purification system to isolate mitochondrial subpopulations.
  JOURNAL   J Cell Sci. 2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693.
  PUBMED    40079232
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693."
COMMENT     Sequence Label: pQCXIP-EGFP-APEX2-V5-Giantin
FEATURES             Location/Qualifiers
     source          1..8893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(882..1078)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(890..1100)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      896..1031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1124..1144
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     1280..1302
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     LTR             1405..1830
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     CDS             complement(2204..2803)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2287..2307)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2784..2803
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2824..3323)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2872..2891)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3265..3288
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(3740..3781)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(3782..4528)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             complement(join(4535..5245,5246..5248,5249..5251))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLK
                     EFVTAAGITLGMDELYK"
     primer_bind     4926..4945
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5185..5206
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5369..5393)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5390..5593)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        5594..5897
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5934..6350)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5950..5966)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5982..6004)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6551..6750
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             6813..6988
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(6989..7192)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        7193..7572
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(7672..7690)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7758..7862
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7863..7931,7932..8723)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8081..8100)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtaagct ttttgcaaaa gcctaggcct
      901 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
      961 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     1021 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     1081 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     1141 ttccacagcc ggatctacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     1201 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     1261 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1321 aaacgcgcga ggcagccagc ttacctcccg gtggtgggtc ggtggtccct gggcaggggt
     1381 ctcccgatcc cggacgagcc cccaaatgaa agacccccgc tgacgggtag tcaatcactc
     1441 agaggagacc ctcccaagga acagcgagac cacaagtcgg atgcaactgc aagagggttt
     1501 attggataca cgggtacccg ggcgactcag tcaatcggag gactggcgcc ccgagtgagg
     1561 ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg
     1621 aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg gcaccctgga
     1681 aacatctgat ggttctctag ggtcgaccct gttccatctg ttcctgacct tgatctgaac
     1741 ttctctattc tcagttatgt atttttccat gccttgcaaa atggcgttac ttaagctagc
     1801 ttgccaaacc tacaggtggg gtctttcatt cccccctttt tctggagact aaataaaatc
     1861 ttttatttta tctatggctc gtactctata ggcttcagct ggtgatattg ttgagtcaaa
     1921 actagagcct ggaccactga tatcctgtct ttaacaaatt ggactaatcg ataagcttat
     1981 ctctacactg gctcgatgga gtcagcctag tgtccctcgg aaagccttga ggtccctttg
     2041 gagcagttct gcactcggcc tctgcagcgc tgcagccctc gactagagtc ggtgggcctc
     2101 gggggcgggt gcggggtcgg cggggccgcc ccgggtggct tcggtcggag ccatggggtc
     2161 gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc
     2221 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     2281 cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc
     2341 cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct
     2401 ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg
     2461 gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca
     2521 tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga
     2581 ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg
     2641 tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccggg tcgacggtgt
     2701 ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt
     2761 cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttgggctgca
     2821 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
     2881 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
     2941 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
     3001 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
     3061 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
     3121 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
     3181 accaaagaac ggagtcgctt ggcgcctaac gctggatgtg gaatgtgtgc gaggccagag
     3241 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
     3301 tgggaaaagc gcctccccta cccggttcac gggtttaaac ctatagatgg cccgtaaaac
     3361 acagaatgag caggacatga atcattagaa agtagatggc tgctagaagt ggcactcggg
     3421 tccgtgaatg acagagtgaa cgcaggactc gcttccatcc aacgccactc cgggtccttc
     3481 gacaactgtt gcttgtaaga tctattaaca gtgcctgctc ctgagtgcca caggagccaa
     3541 tgataggagt ccgggaagag tcccattcac tgtgctctaa ccgtctgatc tgctcctcgg
     3601 ccacagagag agcattctca gcagccactc tttggtctcg ttcttcttcc agcagcttcc
     3661 ttaattcatt cacttcctgt ctggtgttgc atagctgctg ctgagcttca gaaaagcttt
     3721 gctgcggttc tcctcgagag gtgctgtcca ggcccagcag ggggttgggg atgggcttgc
     3781 cggcatcagc aaacccaagc tcggaaagct tttggtgagc ctcagcgtaa tcagcaaaga
     3841 aggcatcttc gtccgctgca tatttgtcaa cgagagggcg gaatacaggg tcagacaaaa
     3901 gagccttgtc agaaggtagc tgaaggagac cttccttctc accactcaac aactccgtga
     3961 agtatgagtt gtcgaaaata agaggattag aggtccaggg accctcaaat ccagaacgct
     4021 ccttgtgtgc agctccaata gtgtgacccc cagatagagc aacgatatct tggtcagtaa
     4081 gccccatagc tttgccaaac acatctctca aatggtcaga acccttagtg ggatcgggca
     4141 agcgaccctc tggtggtggc tcaggcttgt cctctcttcc agggtggaat ggaaccttag
     4201 gtccacccgt gacctcaacg gcaacaacgc cagccaactg gtagaaatcg gcgtagctca
     4261 aaatagggaa ctccgccttg agtggctcca aaagcctaac agcgatgtca agaccgttgt
     4321 tagcgctgtg agccagttcg gcagggtgct tgatggttcc gaagggtcca ccggtcttcg
     4381 tgcccttgtc aaaggttcca gcagagtgga atgccaaacg gagcattaga ggagcgcatc
     4441 tcttctcagc gatgaagcct ctgagcttct tcttcgcctt ctcaacggcg tcctggtaat
     4501 cagcactcac agttgggtaa gactttcctc cggacttgta cagctcgtcc atgccgagag
     4561 tgatcccggc ggcggtcacg aactccttaa ggaccatgtg atcgcgcttc tcgttggggt
     4621 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     4681 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
     4741 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
     4801 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     4861 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     4921 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     4981 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
     5041 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     5101 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     5161 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     5221 cggtgaacag ctcctcgccc ttgctcacca tgcggccgcg gatcaattcc tgcaggtccg
     5281 ttccaatgca ccgttcccgg ccgcggaggc tggatcggtc ccggtgtctt ctatggaggt
     5341 caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga gctctgctta
     5401 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     5461 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     5521 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     5581 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     5641 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     5701 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     5761 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     5821 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     5881 ccgtaagtta tgtaacggac tctagaggat cgatccccca gatcttcaat tccggcgcct
     5941 agagaaggag tgagggctgg ataaagggag gatcgaggcg gggtcgaacg aggaggttca
     6001 agggggagag acggggcgga tggaggaaga ggaggcggag gcttagggtg tacaaagggc
     6061 ttgacccagg gaggggggtc aaaagccaag gcttcccagg tcacgatgta ggggacctgg
     6121 tctgggtgtc catgcgggcc aggtgaaaag accttgatct taacctgggt gatgaggtct
     6181 cggttaaagg tgccgtctcg cggccatccg acgttaaagg ttggccattc tgcagagcag
     6241 aaggtaaccc aacgtctctt cttgacatct accgactggt tgtgagcgat ccgctcgaca
     6301 tctttccagt gacctaaggt caaacttaag ggagtggtaa cagtctggcc ctaattttca
     6361 gacaaataca gaaacacagt cagacagaga caacacagaa cgatgctgca gcgctgcagc
     6421 agacaagacg cgcggcttcg gttccaaacc gaaagcaaaa attcagacgg aggcgggaac
     6481 tgttttaggt tctcgtctcc taccagaacc acatatcctg acggggtcgg attccacatc
     6541 gactcccttc ctcaggtcgg gccacaaaaa cggcccccaa agtccctggg acgtctccca
     6601 gggttgcggc cgggtgttca gaactcgtca gttccaccac gggtccgcca gatacagagc
     6661 tagttagcta actagtacag acgcaggcgc ataacatcaa acatagacac tagacaatcg
     6721 gacagacaca gataagttgc tggccagctt acctcccggt ggtgggtcgg tggtccctgg
     6781 gcaggggtct ccaaatcccg gacgagcccc caaatgaaag acccccgtcg tgggtagtca
     6841 atcactcaga ggagaccctc ccaaggaaca gcgagaccac gattcggatg caaacagcaa
     6901 gaggctttat tgggaatacg ggtacccggg cgacgcagtc tatcggaaga ctggcgcgcc
     6961 gagtgagggg ttgtgggctc ttttattgag ctctgcttat atagacctcc caccgtacac
     7021 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     7081 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     7141 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     7201 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     7261 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     7321 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     7381 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     7441 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     7501 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
     7561 ataatcaatg tcaacatggc ggtaatgttg gacatgagcc aatataaatg tacatattat
     7621 gatatggata caacgtatgc aatggccaag ctaattcttg aagacgaaag ggcctcgtga
     7681 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7741 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7801 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7861 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7921 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7981 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8041 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8101 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8161 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8221 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8281 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8341 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8401 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8461 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8521 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8581 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8641 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8701 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8761 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8821 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8881 tcaaaggatc ttc
//
