LOCUS       sequence-469950-       12739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a fluorescent endosome-localized APEX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Cunningham CN, Van Vranken JG, Larios J, Heyden K, Gygi SP, Rutter J
  TITLE     A dual-purification system to isolate mitochondrial subpopulations.
  JOURNAL   J Cell Sci. 2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693.
  PUBMED    40079232
REFERENCE   2  (bases 1 to 12739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693."
COMMENT     Sequence Label: pQCXIP-EGFP-APEX2-V5-EEA1
FEATURES             Location/Qualifiers
     source          1..12739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(882..1078)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(890..1100)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      896..1031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1124..1144
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     1280..1302
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     LTR             1405..1830
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     CDS             complement(2204..2803)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2287..2307)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2784..2803
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2824..3323)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2872..2891)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3265..3288
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(3341..7576)
                     /codon_start=1
                     /product="human early endosome antigen 1"
                     /label=EEA1
                     /translation="MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQ
                     CMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKW
                     YSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQ
                     KAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIED
                     VAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQ
                     EVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLH
                     QKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQ
                     QQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADL
                     QLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQ
                     IGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQ
                     VTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKE
                     KVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVT
                     AKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDL
                     QQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKQIL
                     KQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKN
                     SKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLK
                     LELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQK
                     TELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQD
                     LKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQD
                     IQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQ
                     LLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEK
                     EVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEAT
                     VQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQ
                     ALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVC
                     DACFNDLQG"
     CDS             complement(7586..7627)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(7628..8374)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             complement(join(8381..9091,9092..9094,9095..9097))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLK
                     EFVTAAGITLGMDELYK"
     primer_bind     8772..8791
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9031..9052
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9215..9239)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9236..9439)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        9440..9743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9780..10196)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(9796..9812)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9828..9850)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    10397..10596
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             10659..10834
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(10835..11038)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        11039..11418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(11518..11536)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11604..11708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11709..11777,11778..12569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11927..11946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtaagct ttttgcaaaa gcctaggcct
      901 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
      961 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     1021 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     1081 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     1141 ttccacagcc ggatctacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     1201 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     1261 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1321 aaacgcgcga ggcagccagc ttacctcccg gtggtgggtc ggtggtccct gggcaggggt
     1381 ctcccgatcc cggacgagcc cccaaatgaa agacccccgc tgacgggtag tcaatcactc
     1441 agaggagacc ctcccaagga acagcgagac cacaagtcgg atgcaactgc aagagggttt
     1501 attggataca cgggtacccg ggcgactcag tcaatcggag gactggcgcc ccgagtgagg
     1561 ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg
     1621 aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg gcaccctgga
     1681 aacatctgat ggttctctag ggtcgaccct gttccatctg ttcctgacct tgatctgaac
     1741 ttctctattc tcagttatgt atttttccat gccttgcaaa atggcgttac ttaagctagc
     1801 ttgccaaacc tacaggtggg gtctttcatt cccccctttt tctggagact aaataaaatc
     1861 ttttatttta tctatggctc gtactctata ggcttcagct ggtgatattg ttgagtcaaa
     1921 actagagcct ggaccactga tatcctgtct ttaacaaatt ggactaatcg ataagcttat
     1981 ctctacactg gctcgatgga gtcagcctag tgtccctcgg aaagccttga ggtccctttg
     2041 gagcagttct gcactcggcc tctgcagcgc tgcagccctc gactagagtc ggtgggcctc
     2101 gggggcgggt gcggggtcgg cggggccgcc ccgggtggct tcggtcggag ccatggggtc
     2161 gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc
     2221 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     2281 cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc
     2341 cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct
     2401 ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg
     2461 gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca
     2521 tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga
     2581 ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg
     2641 tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccggg tcgacggtgt
     2701 ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt
     2761 cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttgggctgca
     2821 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
     2881 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
     2941 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
     3001 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
     3061 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
     3121 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
     3181 accaaagaac ggagtcgctt ggcgcctaac gctggatgtg gaatgtgtgc gaggccagag
     3241 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
     3301 tgggaaaagc gcctccccta cccggttcac gggtttaaac ttatccttgc aagtcattga
     3361 aacatgcatc acagacacga acaggcttct tggaggaagg agttaaggca tttttggctg
     3421 aacattcagc acagaagata tttccacact gtcggcagtg atgccgtctc actgttactg
     3481 aaaagccttt cccacaggcc atacagtttt gtacttcatt gtcttcggcc cactttctat
     3541 tcaacgcttg tgtatgtttg atctgaagtg attggttttc tctgcccagc tcctgcactg
     3601 ctgcagttgt attatccagc tttctttgca attctaatac tttggtttga agcttttcta
     3661 tttcaccttc tcctttaaga catctttcca gtagtgctct cctttcatct tgattattct
     3721 gaaccgttgc ttctaatact gcaatttcac cccgtaagtc atccgtttgt ttctcaagct
     3781 cactaactct ccgttgacta gattgccact ccttcttcac agtgcctaag ttttcattta
     3841 atgctgtaat ctgcatggta agtttagcct cattttcttc atgcttcttc attcctactt
     3901 ccttttcttt tatttcggaa tgcaacttag cttctttctc aataaattct ttcttcagct
     3961 cctcttcttc ctttttcacc tggtctttta gtatctgctg atttctcttc tcctgttcaa
     4021 cagctgcctt cagggagtcc gcttttcctt gaagttctaa tttctgctga attagaagct
     4081 gtttagcatc tttaagattt gttatctcct ttatgctttc tagtttgtgg gacttgagtt
     4141 cttcgtttag tttagtgatt tctttttctt gtctacattt aatttcttct atctctgcca
     4201 atttagactt ctcatttacc agttcttttt ctttcagtga cttttctttc tgaatgtctt
     4261 gtagtgcttt acatcgctcc tgcaattgct gttctttctt tgctgaatcc tgctccaatg
     4321 tagccttggc agtcttcagt tcttgaatca atttattttg atttccaatt tgatttctgt
     4381 ttgaaatcaa gtcctcctgt gctagagaaa gcttctcttc tacagactta agatcttgcc
     4441 tggtggctag aagttcagat tccctcccat agaaatcaga ttgaagctgt ttgaaagttt
     4501 cctgactttt ttcatagttg ttttgtaata ctgatatttt ctctttctct gctgcaagtt
     4561 cctgggctgc ctgtgttaac tgctgctgta gtttattctc aagctctgtc ttctgtaaaa
     4621 cagcaatttt aagctctcct tggagtgctt caatttgttt tttcttctgt tcacttgatt
     4681 gctttagctc atttatgttc ccctgaagtt gttgctcttc tttttcattt tgttttaaag
     4741 tattctgggc ctgtataagt tgttcctgca ttgaattgag ttccaatttc aactgatgag
     4801 aagcctcctt ctctttttca agtgactttt tcagttcctt ctgttcctta agtgtgtttt
     4861 ccatctgcac ttgaagttga tgctttaatt ctttgcaagt tttttccaag tctaatatag
     4921 cggcttttcc tttctgattc tccttttcaa attcactttt agagtttttc aaagaatcag
     4981 aaacttttga tagtttgtcc tttactgtag aaagctctgt cattaaagct tctttctcca
     5041 ttttcacttt ttgtagttct gttactgttg tttgaattct gttattcaat tcctcatgct
     5101 gaatctttgt ttcttgactt aaagtttcaa aatcttgttt caggatttgt ttttcttcct
     5161 cttgcttggt aagcttttgc ttgatacttt caagggcttc agattttttc tgtagatcca
     5221 atcttgtact ggatactatt tccttctcca tttcaagttg tttactcaat tctgtggctc
     5281 tgagctctaa atctgtgttc agctgtcttt gctgttgtag atcttgcaaa gcctgctcct
     5341 tgcttgcttt aacttcaaga ctatcagctt ctagtttctt aatttgacct tctagctctt
     5401 cggttttctg ttctaaagag aggtatttct ctttatattc tttaagatga ctttccagct
     5461 gactgcaatg ttcttgcttg tcttgtaact ttgcagtgac ctgatccaac tgagtagtaa
     5521 tcttatttaa ctcctgctgt ttatcttgta atgcattttg agctgtgtcc aaatgattct
     5581 gaagatcagc tctttgagca gtttttgctg cttctgctga tagtaatagt tcggttttgg
     5641 ctttaatctg tatgtcaagc tgggagacct tctccttgct ttcatttaat tgactattta
     5701 attcattgac actagtttct agggaaagga cacggtcttg tgcagctcta agatgtgcct
     5761 tctgctcttg tacctggtca tgcaaattct cctgggcttg tttatgactt tctgactgat
     5821 tcttcagctt ctctgttagt tgagttactt gctcctgtag tgtatggttt ttttcttgta
     5881 actggttaag aacagcagtc tctccttcac cagcctgaat ttttgcataa agatcttctc
     5941 tttctttttc tagtaatgaa atattttctt tactcttctg taataaagct tcaaggttct
     6001 ggatcttttg gtccttgtcg ccaatttgac gtagaacttg ttccaaatca ttctgagctt
     6061 ctcgaagttt tgccgtggtg ctttgctgaa gagcctgttg ttcttgatgc tgttgctttg
     6121 ttttatctaa ttgatgctgc aattctgtag aatttgtaac tttttccttc aactgctctt
     6181 ctaaccgaga aagtttgagt tgtaaatcag ccacttgttg ttctttatcc atcaactttt
     6241 cacttgaaag ctgtctctgt tccttcagcc taccatgagc ttcccctagt tggcgctctg
     6301 tctctagaag tttgctatgt aattgattaa tttcactttg gagttgtaac ccatgctgct
     6361 ccttttcttc tctctgttgt tgtagctgct taaactccgc ctttagatgc tggtacttgg
     6421 tctctacctc agataattct tctttgagct tttgagtagc ttctcctttt tcacttagtt
     6481 ctacatgtat tctatgcagt gaggtttcag atgcagacaa tcttgactga agctgttgac
     6541 aatctaggtc tttttgatga agggttgcct gaatattctt tttactcaca gattcttcat
     6601 tatgtttctc ctctaactta gtatagtctt gttctttttt cagcaagttt tctgtcaagg
     6661 tctgattttt ttgtgttaat tcattaactg aacttttcag tttttgtagt tcctgtacat
     6721 atacagcaac ttcctggggg cctttggcaa gttcactcct tagctggctt attgtggcct
     6781 ctgagctagc atattgtgac tgcaattttt tgcattcatc tttgagtttt tcagattctc
     6841 gctcacgttc caaggtcatg ttatccatta gtgtttgaac ttggaccagt tctttcttta
     6901 gcacggcaac atcttctata ccaggtctct gaagcagttc cgtcttcaga tcttgaatta
     6961 cagttgcctc tttgtttaat tcttctgtca gacgtgtcac tttttgttca gcagcttctc
     7021 gaagactcct ttcttcatca tactttgact ttatatctgc aatttcagta gcaagttggg
     7081 ctgctttctg ttcaaataag tctttcattt gcttaatatt aaaattttct gtttgggctt
     7141 cttctaattg ctgttccaaa gactgtagtt ctgctgatga atcagtcacc aacccatcag
     7201 gtttggcctc ttgctgctgc agcccttgat atttttctaa ttccttcttt aattcttccg
     7261 agtaccattt ttcttcctta agtgaagcct gtaggtcttg gacctcttgt ctgagcagtg
     7321 ttacatcatc tcgcttcaaa gcaagattag actctcctcc atgacctgag tcattaccag
     7381 catcatgaac agcttcataa tgtttgaaaa gttcatcagc agatccaaga gatttcatac
     7441 actggggaca tatgaaacct tctgaagagc tttcattatt gacgtccact gtgtttatag
     7501 gagttgctga tgaatctaaa tcagaacctt gagagccaac tctcccagga gtcctctgta
     7561 aaatcctcct taacatttct cgagaggtgc tgtccaggcc cagcaggggg ttggggatgg
     7621 gcttgccggc atcagcaaac ccaagctcgg aaagcttttg gtgagcctca gcgtaatcag
     7681 caaagaaggc atcttcgtcc gctgcatatt tgtcaacgag agggcggaat acagggtcag
     7741 acaaaagagc cttgtcagaa ggtagctgaa ggagaccttc cttctcacca ctcaacaact
     7801 ccgtgaagta tgagttgtcg aaaataagag gattagaggt ccagggaccc tcaaatccag
     7861 aacgctcctt gtgtgcagct ccaatagtgt gacccccaga tagagcaacg atatcttggt
     7921 cagtaagccc catagctttg ccaaacacat ctctcaaatg gtcagaaccc ttagtgggat
     7981 cgggcaagcg accctctggt ggtggctcag gcttgtcctc tcttccaggg tggaatggaa
     8041 ccttaggtcc acccgtgacc tcaacggcaa caacgccagc caactggtag aaatcggcgt
     8101 agctcaaaat agggaactcc gccttgagtg gctccaaaag cctaacagcg atgtcaagac
     8161 cgttgttagc gctgtgagcc agttcggcag ggtgcttgat ggttccgaag ggtccaccgg
     8221 tcttcgtgcc cttgtcaaag gttccagcag agtggaatgc caaacggagc attagaggag
     8281 cgcatctctt ctcagcgatg aagcctctga gcttcttctt cgccttctca acggcgtcct
     8341 ggtaatcagc actcacagtt gggtaagact ttcctccgga cttgtacagc tcgtccatgc
     8401 cgagagtgat cccggcggcg gtcacgaact ccttaaggac catgtgatcg cgcttctcgt
     8461 tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
     8521 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
     8581 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
     8641 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
     8701 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
     8761 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
     8821 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
     8881 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
     8941 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
     9001 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
     9061 ccaccccggt gaacagctcc tcgcccttgc tcaccatgcg gccgcggatc aattcctgca
     9121 ggtccgttcc aatgcaccgt tcccggccgc ggaggctgga tcggtcccgg tgtcttctat
     9181 ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc
     9241 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     9301 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     9361 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     9421 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     9481 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     9541 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     9601 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     9661 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     9721 catttaccgt aagttatgta acggactcta gaggatcgat cccccagatc ttcaattccg
     9781 gcgcctagag aaggagtgag ggctggataa agggaggatc gaggcggggt cgaacgagga
     9841 ggttcaaggg ggagagacgg ggcggatgga ggaagaggag gcggaggctt agggtgtaca
     9901 aagggcttga cccagggagg ggggtcaaaa gccaaggctt cccaggtcac gatgtagggg
     9961 acctggtctg ggtgtccatg cgggccaggt gaaaagacct tgatcttaac ctgggtgatg
    10021 aggtctcggt taaaggtgcc gtctcgcggc catccgacgt taaaggttgg ccattctgca
    10081 gagcagaagg taacccaacg tctcttcttg acatctaccg actggttgtg agcgatccgc
    10141 tcgacatctt tccagtgacc taaggtcaaa cttaagggag tggtaacagt ctggccctaa
    10201 ttttcagaca aatacagaaa cacagtcaga cagagacaac acagaacgat gctgcagcgc
    10261 tgcagcagac aagacgcgcg gcttcggttc caaaccgaaa gcaaaaattc agacggaggc
    10321 gggaactgtt ttaggttctc gtctcctacc agaaccacat atcctgacgg ggtcggattc
    10381 cacatcgact cccttcctca ggtcgggcca caaaaacggc ccccaaagtc cctgggacgt
    10441 ctcccagggt tgcggccggg tgttcagaac tcgtcagttc caccacgggt ccgccagata
    10501 cagagctagt tagctaacta gtacagacgc aggcgcataa catcaaacat agacactaga
    10561 caatcggaca gacacagata agttgctggc cagcttacct cccggtggtg ggtcggtggt
    10621 ccctgggcag gggtctccaa atcccggacg agcccccaaa tgaaagaccc ccgtcgtggg
    10681 tagtcaatca ctcagaggag accctcccaa ggaacagcga gaccacgatt cggatgcaaa
    10741 cagcaagagg ctttattggg aatacgggta cccgggcgac gcagtctatc ggaagactgg
    10801 cgcgccgagt gaggggttgt gggctctttt attgagctct gcttatatag acctcccacc
    10861 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
    10921 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
    10981 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
    11041 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
    11101 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
    11161 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
    11221 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
    11281 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
    11341 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
    11401 tagtcaataa tcaatgtcaa catggcggta atgttggaca tgagccaata taaatgtaca
    11461 tattatgata tggatacaac gtatgcaatg gccaagctaa ttcttgaaga cgaaagggcc
    11521 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    11581 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    11641 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    11701 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    11761 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    11821 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    11881 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    11941 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    12001 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    12061 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    12121 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    12181 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    12241 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    12301 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    12361 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    12421 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    12481 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    12541 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    12601 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    12661 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    12721 aaaagatcaa aggatcttc
//
