LOCUS       sequence-469951-        9817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a fluorescent lysosome-localized APEX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Cunningham CN, Van Vranken JG, Larios J, Heyden K, Gygi SP, Rutter J
  TITLE     A dual-purification system to isolate mitochondrial subpopulations.
  JOURNAL   J Cell Sci. 2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693.
  PUBMED    40079232
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693."
COMMENT     Sequence Label: pQCXIP-LAMP1-EGFP-APEX2-V5
FEATURES             Location/Qualifiers
     source          1..9817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(882..1078)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(890..1100)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      896..1031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1124..1144
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     1280..1302
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     LTR             1405..1830
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     CDS             complement(2204..2803)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2287..2307)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2784..2803
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2824..3323)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2872..2891)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3265..3288
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(3344..3385)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(3386..4132)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             complement(join(4139..4849,4850..4852,4853..4855))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLK
                     EFVTAAGITLGMDELYK"
     primer_bind     4530..4549
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4789..4810
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4852..4861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(4925..6091,6092..6175))
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
     primer_bind     complement(6293..6317)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6314..6517)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        6518..6821
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6858..7274)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(6874..6890)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6906..6928)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    7475..7674
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             7737..7912
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(7913..8116)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        8117..8496
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(8596..8614)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8682..8786
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8787..8855,8856..9647)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9005..9024)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtaagct ttttgcaaaa gcctaggcct
      901 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
      961 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     1021 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     1081 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     1141 ttccacagcc ggatctacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     1201 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     1261 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1321 aaacgcgcga ggcagccagc ttacctcccg gtggtgggtc ggtggtccct gggcaggggt
     1381 ctcccgatcc cggacgagcc cccaaatgaa agacccccgc tgacgggtag tcaatcactc
     1441 agaggagacc ctcccaagga acagcgagac cacaagtcgg atgcaactgc aagagggttt
     1501 attggataca cgggtacccg ggcgactcag tcaatcggag gactggcgcc ccgagtgagg
     1561 ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg
     1621 aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg gcaccctgga
     1681 aacatctgat ggttctctag ggtcgaccct gttccatctg ttcctgacct tgatctgaac
     1741 ttctctattc tcagttatgt atttttccat gccttgcaaa atggcgttac ttaagctagc
     1801 ttgccaaacc tacaggtggg gtctttcatt cccccctttt tctggagact aaataaaatc
     1861 ttttatttta tctatggctc gtactctata ggcttcagct ggtgatattg ttgagtcaaa
     1921 actagagcct ggaccactga tatcctgtct ttaacaaatt ggactaatcg ataagcttat
     1981 ctctacactg gctcgatgga gtcagcctag tgtccctcgg aaagccttga ggtccctttg
     2041 gagcagttct gcactcggcc tctgcagcgc tgcagccctc gactagagtc ggtgggcctc
     2101 gggggcgggt gcggggtcgg cggggccgcc ccgggtggct tcggtcggag ccatggggtc
     2161 gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc
     2221 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     2281 cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc
     2341 cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct
     2401 ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg
     2461 gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca
     2521 tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga
     2581 ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg
     2641 tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccggg tcgacggtgt
     2701 ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt
     2761 cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttgggctgca
     2821 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
     2881 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
     2941 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
     3001 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
     3061 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
     3121 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
     3181 accaaagaac ggagtcgctt ggcgcctaac gctggatgtg gaatgtgtgc gaggccagag
     3241 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
     3301 tgggaaaagc gcctccccta cccggttcac gggtttaaac tcaggtgctg tccaggccca
     3361 gcagggggtt ggggatgggc ttgccggcat cagcaaaccc aagctcggaa agcttttggt
     3421 gagcctcagc gtaatcagca aagaaggcat cttcgtccgc tgcatatttg tcaacgagag
     3481 ggcggaatac agggtcagac aaaagagcct tgtcagaagg tagctgaagg agaccttcct
     3541 tctcaccact caacaactcc gtgaagtatg agttgtcgaa aataagagga ttagaggtcc
     3601 agggaccctc aaatccagaa cgctccttgt gtgcagctcc aatagtgtga cccccagata
     3661 gagcaacgat atcttggtca gtaagcccca tagctttgcc aaacacatct ctcaaatggt
     3721 cagaaccctt agtgggatcg ggcaagcgac cctctggtgg tggctcaggc ttgtcctctc
     3781 ttccagggtg gaatggaacc ttaggtccac ccgtgacctc aacggcaaca acgccagcca
     3841 actggtagaa atcggcgtag ctcaaaatag ggaactccgc cttgagtggc tccaaaagcc
     3901 taacagcgat gtcaagaccg ttgttagcgc tgtgagccag ttcggcaggg tgcttgatgg
     3961 ttccgaaggg tccaccggtc ttcgtgccct tgtcaaaggt tccagcagag tggaatgcca
     4021 aacggagcat tagaggagcg catctcttct cagcgatgaa gcctctgagc ttcttcttcg
     4081 ccttctcaac ggcgtcctgg taatcagcac tcacagttgg gtaagacttt cctccggact
     4141 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc ttaaggacca
     4201 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
     4261 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     4321 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     4381 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     4441 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     4501 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     4561 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     4621 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     4681 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     4741 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     4801 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg
     4861 cgatggatcc cgggcccgcg gtaccgtcga ccgcgccggt ggagcctgtg gagccggtgg
     4921 agccgatagt ctggtagcct gcgtgactcc tcttcctgcc gacgaggtag gcgatgagga
     4981 cgatgaggac cagccccgcc agggcaccac ccacagcgat ggggatcagc atgctgttct
     5041 cgtccagcag acactcctcc acagagccaa actggccacc ttccaccttg aaagcctgga
     5101 cccacacttt gaatatattg actgaaaacg ccttcgtgac acggacgtgc tcctccgcgt
     5161 tgcacttgta ggaattgccg actgtggcct gcagcgctcg cagggagccg ttggcagctt
     5221 taaaggcggg gtctctggcg tcaggaagaa ttgtattcaa ctggattcct tgtaggaaaa
     5281 accggctaga acttgcattc atcccgaact ggaagagcag gacggtggtg ccctcgctgt
     5341 gcagctccag agtcaccagg tgggcgccgc agctcccgct ggccgaggtc ttgttggggt
     5401 tgatgttgag aagccttgtc accgtcgtgt tgtccttcct ctcataggtg aggttcagct
     5461 gcagccccat gctggccagc aggcaggtcc cgttggtgcc gctcacgttg tacttgtcca
     5521 cagaggggct cttgggcacg ggtgagggcg aggggctggg tggcgcaggg ggcgctgtgg
     5581 ttggggaagg cctgtcttgt tcacagcgtg tctctcccct gctgaagctg ctgttggaaa
     5641 ggtacgcctg gatggtggca tcatggagcg ttacggtcac gttgttcatg tggacctggg
     5701 tgccactaac acatctgtat tttttatcta tatctgccct gatgtcagtt atagattcca
     5761 cagtcttgat ttctttggag ctcgcattgg ggaaaaggtg tgtgtctgac aagttataaa
     5821 caaaactcat gagctggacg ctgtaacgtg ttgcatttct cgtgaaattg agagtgagtg
     5881 tatgtcctct tccaaaagca atcacgagac tggggtcaga agtgttctct tttccacagg
     5941 agctgcggtt gagcaccact gtggcatctg atggcaggtc aaaggtcatg ttcttagggc
     6001 cactcttggt gtcgtagttc actgagaagg cagcagagaa gttggccatt atgcacgcgg
     6061 tcccgttgcc atttttcacc ataaacattg ctgctgacgc acaatgcatg aggccgagca
     6121 gcagcaacag cagtagcagc agcaggggtc gccgggcgct gccgggggcc gccatgcggc
     6181 cgcggatcaa ttcctgcagg tccgttccaa tgcaccgttc ccggccgcgg aggctggatc
     6241 ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag gcgatctgac
     6301 ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
     6361 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
     6421 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
     6481 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
     6541 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
     6601 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
     6661 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
     6721 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     6781 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac ggactctaga ggatcgatcc
     6841 cccagatctt caattccggc gcctagagaa ggagtgaggg ctggataaag ggaggatcga
     6901 ggcggggtcg aacgaggagg ttcaaggggg agagacgggg cggatggagg aagaggaggc
     6961 ggaggcttag ggtgtacaaa gggcttgacc cagggagggg ggtcaaaagc caaggcttcc
     7021 caggtcacga tgtaggggac ctggtctggg tgtccatgcg ggccaggtga aaagaccttg
     7081 atcttaacct gggtgatgag gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta
     7141 aaggttggcc attctgcaga gcagaaggta acccaacgtc tcttcttgac atctaccgac
     7201 tggttgtgag cgatccgctc gacatctttc cagtgaccta aggtcaaact taagggagtg
     7261 gtaacagtct ggccctaatt ttcagacaaa tacagaaaca cagtcagaca gagacaacac
     7321 agaacgatgc tgcagcgctg cagcagacaa gacgcgcggc ttcggttcca aaccgaaagc
     7381 aaaaattcag acggaggcgg gaactgtttt aggttctcgt ctcctaccag aaccacatat
     7441 cctgacgggg tcggattcca catcgactcc cttcctcagg tcgggccaca aaaacggccc
     7501 ccaaagtccc tgggacgtct cccagggttg cggccgggtg ttcagaactc gtcagttcca
     7561 ccacgggtcc gccagataca gagctagtta gctaactagt acagacgcag gcgcataaca
     7621 tcaaacatag acactagaca atcggacaga cacagataag ttgctggcca gcttacctcc
     7681 cggtggtggg tcggtggtcc ctgggcaggg gtctccaaat cccggacgag cccccaaatg
     7741 aaagaccccc gtcgtgggta gtcaatcact cagaggagac cctcccaagg aacagcgaga
     7801 ccacgattcg gatgcaaaca gcaagaggct ttattgggaa tacgggtacc cgggcgacgc
     7861 agtctatcgg aagactggcg cgccgagtga ggggttgtgg gctcttttat tgagctctgc
     7921 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     7981 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     8041 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     8101 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     8161 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     8221 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     8281 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     8341 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     8401 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     8461 ttgattacta ttaataacta gtcaataatc aatgtcaaca tggcggtaat gttggacatg
     8521 agccaatata aatgtacata ttatgatatg gatacaacgt atgcaatggc caagctaatt
     8581 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     8641 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8701 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8761 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8821 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8881 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8941 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9001 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     9061 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9121 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9181 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9241 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9301 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
     9361 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9421 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9481 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9541 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9601 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9661 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9721 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9781 gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
