LOCUS       sequence-469954-        8605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a fluorescent Endoplasmic Reticulum-localized APEX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Cunningham CN, Van Vranken JG, Larios J, Heyden K, Gygi SP, Rutter J
  TITLE     A dual-purification system to isolate mitochondrial subpopulations.
  JOURNAL   J Cell Sci. 2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693.
  PUBMED    40079232
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693."
COMMENT     Sequence Label: pQCXIP-C1(1-29)-EGFP-APEX2-V5
FEATURES             Location/Qualifiers
     source          1..8605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(882..1078)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(890..1100)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      896..1031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1124..1144
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     1280..1302
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     LTR             1405..1830
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     CDS             complement(2204..2803)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2287..2307)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2784..2803
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2824..3323)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2872..2891)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3265..3288
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(3344..3385)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(3386..4132)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             complement(join(4139..4849,4850..4852,4853..4855))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4139..4849,4850..4852,4853..4855))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4181..4202)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4530..4549
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4789..4810
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4954..4963
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5081..5105)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5102..5305)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        5306..5609
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5646..6062)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5662..5678)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5694..5716)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6263..6462
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             6525..6700
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(6701..6904)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        6905..7284
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(7384..7402)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7470..7574
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7575..7643,7644..8435)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7793..7812)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtaagct ttttgcaaaa gcctaggcct
      901 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
      961 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     1021 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     1081 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     1141 ttccacagcc ggatctacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     1201 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     1261 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1321 aaacgcgcga ggcagccagc ttacctcccg gtggtgggtc ggtggtccct gggcaggggt
     1381 ctcccgatcc cggacgagcc cccaaatgaa agacccccgc tgacgggtag tcaatcactc
     1441 agaggagacc ctcccaagga acagcgagac cacaagtcgg atgcaactgc aagagggttt
     1501 attggataca cgggtacccg ggcgactcag tcaatcggag gactggcgcc ccgagtgagg
     1561 ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg
     1621 aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg gcaccctgga
     1681 aacatctgat ggttctctag ggtcgaccct gttccatctg ttcctgacct tgatctgaac
     1741 ttctctattc tcagttatgt atttttccat gccttgcaaa atggcgttac ttaagctagc
     1801 ttgccaaacc tacaggtggg gtctttcatt cccccctttt tctggagact aaataaaatc
     1861 ttttatttta tctatggctc gtactctata ggcttcagct ggtgatattg ttgagtcaaa
     1921 actagagcct ggaccactga tatcctgtct ttaacaaatt ggactaatcg ataagcttat
     1981 ctctacactg gctcgatgga gtcagcctag tgtccctcgg aaagccttga ggtccctttg
     2041 gagcagttct gcactcggcc tctgcagcgc tgcagccctc gactagagtc ggtgggcctc
     2101 gggggcgggt gcggggtcgg cggggccgcc ccgggtggct tcggtcggag ccatggggtc
     2161 gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc
     2221 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     2281 cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc
     2341 cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct
     2401 ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg
     2461 gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca
     2521 tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga
     2581 ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg
     2641 tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccggg tcgacggtgt
     2701 ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt
     2761 cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttgggctgca
     2821 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
     2881 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
     2941 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
     3001 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
     3061 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
     3121 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
     3181 accaaagaac ggagtcgctt ggcgcctaac gctggatgtg gaatgtgtgc gaggccagag
     3241 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
     3301 tgggaaaagc gcctccccta cccggttcac gggtttaaac tcaggtgctg tccaggccca
     3361 gcagggggtt ggggatgggc ttgccggcat cagcaaaccc aagctcggaa agcttttggt
     3421 gagcctcagc gtaatcagca aagaaggcat cttcgtccgc tgcatatttg tcaacgagag
     3481 ggcggaatac agggtcagac aaaagagcct tgtcagaagg tagctgaagg agaccttcct
     3541 tctcaccact caacaactcc gtgaagtatg agttgtcgaa aataagagga ttagaggtcc
     3601 agggaccctc aaatccagaa cgctccttgt gtgcagctcc aatagtgtga cccccagata
     3661 gagcaacgat atcttggtca gtaagcccca tagctttgcc aaacacatct ctcaaatggt
     3721 cagaaccctt agtgggatcg ggcaagcgac cctctggtgg tggctcaggc ttgtcctctc
     3781 ttccagggtg gaatggaacc ttaggtccac ccgtgacctc aacggcaaca acgccagcca
     3841 actggtagaa atcggcgtag ctcaaaatag ggaactccgc cttgagtggc tccaaaagcc
     3901 taacagcgat gtcaagaccg ttgttagcgc tgtgagccag ttcggcaggg tgcttgatgg
     3961 ttccgaaggg tccaccggtc ttcgtgccct tgtcaaaggt tccagcagag tggaatgcca
     4021 aacggagcat tagaggagcg catctcttct cagcgatgaa gcctctgagc ttcttcttcg
     4081 ccttctcaac ggcgtcctgg taatcagcac tcacagttgg gtaagacttt cctccggact
     4141 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     4201 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
     4261 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     4321 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     4381 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     4441 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     4501 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     4561 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     4621 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     4681 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     4741 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     4801 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatgcgtc
     4861 cgccgcttcc tgatcccccg cctccgtagg actgcttcca caggctcagc agcagcagac
     4921 agctcaggca cagccccagc acgaccacag ggtccatggt ggcgcggccg cggatcaatt
     4981 cctgcaggtc cgttccaatg caccgttccc ggccgcggag gctggatcgg tcccggtgtc
     5041 ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac
     5101 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     5161 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     5221 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     5281 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     5341 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     5401 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     5461 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     5521 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     5581 gcgggccatt taccgtaagt tatgtaacgg actctagagg atcgatcccc cagatcttca
     5641 attccggcgc ctagagaagg agtgagggct ggataaaggg aggatcgagg cggggtcgaa
     5701 cgaggaggtt caagggggag agacggggcg gatggaggaa gaggaggcgg aggcttaggg
     5761 tgtacaaagg gcttgaccca gggagggggg tcaaaagcca aggcttccca ggtcacgatg
     5821 taggggacct ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat cttaacctgg
     5881 gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc cgacgttaaa ggttggccat
     5941 tctgcagagc agaaggtaac ccaacgtctc ttcttgacat ctaccgactg gttgtgagcg
     6001 atccgctcga catctttcca gtgacctaag gtcaaactta agggagtggt aacagtctgg
     6061 ccctaatttt cagacaaata cagaaacaca gtcagacaga gacaacacag aacgatgctg
     6121 cagcgctgca gcagacaaga cgcgcggctt cggttccaaa ccgaaagcaa aaattcagac
     6181 ggaggcggga actgttttag gttctcgtct cctaccagaa ccacatatcc tgacggggtc
     6241 ggattccaca tcgactccct tcctcaggtc gggccacaaa aacggccccc aaagtccctg
     6301 ggacgtctcc cagggttgcg gccgggtgtt cagaactcgt cagttccacc acgggtccgc
     6361 cagatacaga gctagttagc taactagtac agacgcaggc gcataacatc aaacatagac
     6421 actagacaat cggacagaca cagataagtt gctggccagc ttacctcccg gtggtgggtc
     6481 ggtggtccct gggcaggggt ctccaaatcc cggacgagcc cccaaatgaa agacccccgt
     6541 cgtgggtagt caatcactca gaggagaccc tcccaaggaa cagcgagacc acgattcgga
     6601 tgcaaacagc aagaggcttt attgggaata cgggtacccg ggcgacgcag tctatcggaa
     6661 gactggcgcg ccgagtgagg ggttgtgggc tcttttattg agctctgctt atatagacct
     6721 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
     6781 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
     6841 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
     6901 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     6961 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     7021 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     7081 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     7141 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     7201 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     7261 aataactagt caataatcaa tgtcaacatg gcggtaatgt tggacatgag ccaatataaa
     7321 tgtacatatt atgatatgga tacaacgtat gcaatggcca agctaattct tgaagacgaa
     7381 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7441 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7501 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7561 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7621 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7681 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7741 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7801 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7861 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7921 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7981 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8041 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8101 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     8161 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8221 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8281 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8341 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8401 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8461 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8521 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8581 ccgtagaaaa gatcaaagga tcttc
//
