LOCUS       sequence-469955-        2757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet-I N-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3b_029_mScarlet-I
FEATURES             Location/Qualifiers
     source          1..2757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      725..782
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(806..823)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(977..1565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1057..1076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1748..1842)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1863..2522)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2437..2456
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2684..2702
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2706..2749)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ggtctcacca tggtttcaaa aggtgaagca gtaattaaag aatttatgcg tttcaaagtt
       61 catatggaag gttctatgaa tggtcacgaa tttgaaattg aaggtgaagg tgaaggtcgt
      121 ccatatgaag gtacacaaac tgctaaatta aaagtaacaa aaggtggtcc attacctttt
      181 agttgggata ttttatctcc acaatttatg tatggttcac gtgcatttac aaaacaccca
      241 gcagatattc ctgactatta caaacaaagt tttccagaag gttttaaatg ggaacgtgta
      301 atgaattttg aagatggtgg tgctgttaca gtaacacaag atacatcatt agaagatggt
      361 actttaattt ataaagttaa attacgtggt acaaatttcc ctccagatgg tccagtaatg
      421 caaaaaaaaa caatgggttg ggaagcttca actgaacgtt tatatccaga agatggtgtt
      481 ttaaaaggtg acattaaaat ggctttacgt ttaaaagatg gtggtcgtta tttagcagat
      541 ttcaaaacta catacaaagc taaaaaacct gttcaaatgc caggtgctta caatgttgac
      601 cgtaaattag atattacaag tcataacgaa gattacacag ttgtagaaca atacgaacgt
      661 tcagaaggtc gtcattcaac tggtggtatg gatgaattat ataaaggaat gcgagaccct
      721 gcagtccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa
      781 gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      961 ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac
     1021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1381 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1621 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1681 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga
     1741 ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     1801 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
     1861 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     1921 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     1981 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     2041 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     2101 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     2161 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     2221 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     2281 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     2341 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     2401 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     2461 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     2521 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     2581 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc
     2641 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     2701 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattc
//
