LOCUS       sequence-469956-        2778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCerulean N-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3b_030_mCerulean
FEATURES             Location/Qualifiers
     source          1..2778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      746..803
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(827..844)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(998..1586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1078..1097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1769..1863)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1884..2543)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2458..2477
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2705..2723
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2727..2770)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ggtctcacca tggtttctaa aggtgaagaa ttattcactg gtgttgtacc aattttagtt
       61 gaattagatg gtgatgttaa cggtcacaaa ttctctgttt caggtgaagg tgaaggtgat
      121 gctacatacg gtaaattaac attaaaattc atttgtacta caggtaaatt accagtacca
      181 tggccaacat tagttacaac tttaacttgg ggtgtacaat gtttcgctcg ttacccagat
      241 cacatgaaac aacacgattt tttcaaatca gctatgcctg aaggttacgt acaagaacgt
      301 actatttttt tcaaagacga tggtaactac aaaacacgtg ctgaagttaa attcgaaggt
      361 gatacattag taaaccgtat tgaattaaaa ggtattgact tcaaagaaga tggtaacatt
      421 ttaggtcaca aattagaata caacgctatt tctgataacg tttacattac agctgacaaa
      481 caaaaaaacg gtattaaagc taacttcaaa attcgtcaca acattgaaga tggttcagtt
      541 caattagctg atcactacca acaaaacact ccaattggtg atggtccagt attattacct
      601 gataaccact acttatctac tcaatcaaaa ttatctaaag atccaaacga aaaacgtgat
      661 cacatggtat tattagaatt cgtaactgct gctggtatta ctttaggtat ggatgaatta
      721 tacaaaggaa tgcgagaccc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc
      781 ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact
      841 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      901 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      961 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg
     1021 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1081 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1141 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1201 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1261 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1321 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1381 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1441 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1501 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1621 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1681 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1741 aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac
     1801 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     1861 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     1921 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     1981 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     2041 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     2101 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     2161 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     2221 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     2281 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     2341 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     2401 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     2461 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     2521 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     2581 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     2641 ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     2701 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     2761 ggatgatttc tggaattc
//
