LOCUS       sequence-469962-       10623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing ADAR1 p110.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Tang S, Stokasimov E, Cui Y, Pellman D
  TITLE     Breakage of cytoplasmic chromosomes by pathological DNA base 
            excision repair.
  JOURNAL   Nature. 2022 Jun;606(7916):930-936. doi: 10.1038/s41586-022-04767-1.
            Epub 2022 Apr 27.
  PUBMED    35477155
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04767-1"; journalName: "Nature"; date: 
            "2022-06"; volume: "606"; issue: "7916"; pages: "930-936"
COMMENT     Sequence Label: pHAGE Flag HA ADAR1 p110
FEATURES             Location/Qualifiers
     source          1..10623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1036..1041,1042..1059,1060..1066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1079..1101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1098..1114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1164..1183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3436..3553
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3605..3908
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3909..4112
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4063..4083
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4109..4133
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4209..4218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4218..4241
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4254..4280
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4296..4320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7153..7177)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7190..7757
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7207..7759
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7351..7368)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7578..7597
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7771..8370
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7771..7790)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8267..8287
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8387..8975
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8440..8460)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8858..8869)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9050..9283
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9402..9420)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9488..9592
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9593..9661,9662..10453)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9811..9830)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattaatt cacaaatggc
     3421 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     3481 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     3541 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga ctagtggagt
     3601 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3661 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3721 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3781 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     3841 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     3901 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     3961 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4021 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4081 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     4141 gacgccatcc acgctgtttt gacctccata gaagacaccg gcggccgcag atctctcgag
     4201 gttgatctgc caccatggac tacaaggatg acgatgacaa gctcgatgga ggatacccat
     4261 acgatgttcc agattacgct gctcgaggtt atcaaacaag tttgtacaaa aaagcaggct
     4321 ccgcggccgc ccccttcacc atggccgaga tcaaggagaa aatctgcgac tatctcttca
     4381 atgtgtctga ctcctctgcc ctgaatttgg ctaaaaatat tggccttacc aaggcccgag
     4441 atataaatgc tgtgctaatt gacatggaaa ggcaggggga tgtctataga caagggacaa
     4501 cccctcccat atggcatttg acagacaaga agcgagagag gatgcaaatc aagagaaata
     4561 cgaacagtgt tcctgaaacc gctccagctg caatccctga gaccaaaaga aacgcagagt
     4621 tcctcacctg taatataccc acatcaaatg cctcaaataa catggtaacc acagaaaaag
     4681 tggagaatgg gcaggaacct gtcataaagt tagaaaacag gcaagaggcc agaccagaac
     4741 cagcaagact gaaaccacct gttcattaca atggcccctc aaaagcaggg tatgttgact
     4801 ttgaaaatgg ccagtgggcc acagatgaca tcccagatga cttgaatagt atccgcgcag
     4861 caccaggtga gtttcgagcc atcatggaga tgccctcctt ctacagtcat ggcttgccac
     4921 ggtgttcacc ctacaagaaa ctgacagagt gccagctgaa gaaccccatc agcgggctgt
     4981 tagaatatgc ccagttcgct agtcaaacct gtgagttcaa catgatagag cagagtggac
     5041 caccccatga acctcgattt aaattccagg ttgtcatcaa tggccgagag tttcccccag
     5101 ctgaagctgg aagcaagaaa gtggccaagc aggatgcagc tatgaaagcc atgacaattc
     5161 tgctagagga agccaaagcc aaggacagtg gaaaatcaga agaatcatcc cactattcca
     5221 cagagaaaga atcagagaag actgcagagt cccagacccc caccccttca gccacatcct
     5281 tcttttctgg gaagagcccc gtcaccacac tgcttgagtg tatgcacaaa ttggggaact
     5341 cctgtgaatt ccgtctcctg tccaaagaag gccctgccca tgaacccaag ttccaatact
     5401 gtgttgcagt gggagcccaa actttcccca gtgtgagtgc tcccagcaag aaagtggcaa
     5461 agcagatggc cgcagaggaa gccatgaagg ccctgcatgg ggaggcgacc aactccatgg
     5521 cttctgataa ccagcctgaa ggtatgatct cagagtcact tgataacttg gaatccatga
     5581 tgcccaacaa ggtcaggaag attggcgagc tcgtgagata cctgaacacc aaccctgtgg
     5641 gtggcctttt ggagtacgcc cgctcccatg gctttgctgc tgaattcaag ttggtcgacc
     5701 agtccggacc tcctcacgag cccaagttcg tttaccaagc aaaagttggg ggtcgctggt
     5761 tcccagccgt ctgcgcacac agcaagaagc aaggcaagca ggaagcagca gatgcggctc
     5821 tccgtgtctt gattggggag aacgagaagg cagaacgcat gggtttcaca gaggtaaccc
     5881 cagtgacagg ggccagtctc agaagaacta tgctcctcct ctcaaggtcc ccagaagcac
     5941 agccaaagac actccctctc actggcagca ccttccatga ccagatagcc atgctgagcc
     6001 accggtgctt caacactctg actaacagct tccagccctc cttgctcggc cgcaagattc
     6061 tggccgccat cattatgaaa aaagactctg aggacatggg tgtcgtcgtc agcttgggaa
     6121 cagggaatcg ctgtgtaaaa ggagattctc tcagcctaaa aggagaaact gtcaatgact
     6181 gccatgcaga aataatctcc cggagaggct tcatcaggtt tctctacagt gagttaatga
     6241 aatacaactc ccagactgcg aaggatagta tatttgaacc tgctaaggga ggagaaaagc
     6301 tccaaataaa aaagactgtg tcattccatc tgtatatcag cactgctccg tgtggagatg
     6361 gcgccctctt tgacaagtcc tgcagcgacc gtgctatgga aagcacagaa tcccgccact
     6421 accctgtctt cgagaatccc aaacaaggaa agctccgcac caaggtggag aacggagaag
     6481 gcacaatccc tgtggaatcc agtgacattg tgcctacgtg ggatggcatt cggctcgggg
     6541 agagactccg taccatgtcc tgtagtgaca aaatcctacg ctggaacgtg ctgggcctgc
     6601 aaggggcact gttgacccac ttcctgcagc ccatttatct caaatctgtc acattgggtt
     6661 accttttcag ccaagggcat ctgacccgtg ctatttgctg tcgtgtgaca agagatggga
     6721 gtgcatttga ggatggacta cgacatccct ttattgtcaa ccaccccaag gttggcagag
     6781 tcagcatata tgattccaaa aggcaatccg ggaagactaa ggagacaagc gtcaactggt
     6841 gtctggctga tggctatgac ctggagatcc tggacggtac cagaggcact gtggatgggc
     6901 cacggaatga attgtcccgg gtctccaaaa agaacatttt tcttctattt aagaagctct
     6961 gctccttccg ttaccgcagg gatctactga gactctccta tggtgaggcc aagaaagctg
     7021 cccgtgacta cgagacggcc aagaactact tcaaaaaagg cctgaaggat atgggctatg
     7081 ggaactggat tagcaaaccc caggaggaaa agaactttta tctctgccca gtatagaagg
     7141 gtgggcgcgc cgacccagct ttcttgtaca aagtggttcg ataacgaatt ccgccccccc
     7201 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     7261 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     7321 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     7381 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     7441 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     7501 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     7561 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     7621 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     7681 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     7741 gaaaaacacg atgataatat ggccacaacc atgaccgagt acaagcccac ggtgcgcctc
     7801 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     7861 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     7921 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     7981 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     8041 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     8101 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     8161 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     8221 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     8281 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     8341 tggtgcatga cccgcaagcc cggtgcctga tgcatcgatg gatcctaatc aacctctgga
     8401 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     8461 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     8521 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     8581 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     8641 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     8701 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     8761 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     8821 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     8881 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     8941 gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact
     9001 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     9061 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     9121 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9181 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9241 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     9301 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg
     9361 ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt
     9421 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     9481 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9541 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9601 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9661 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9721 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9781 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     9841 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9901 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9961 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    10021 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    10081 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    10141 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    10201 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    10261 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    10321 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    10381 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10441 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10501 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10561 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10621 ttc
//
