LOCUS       sequence-469969-       10519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral expression of C-term SETX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10519)
  AUTHORS   Tang S, Stokasimov E, Cui Y, Pellman D
  TITLE     Breakage of cytoplasmic chromosomes by pathological DNA base 
            excision repair.
  JOURNAL   Nature. 2022 Jun;606(7916):930-936. doi: 10.1038/s41586-022-04767-1.
            Epub 2022 Apr 27.
  PUBMED    35477155
REFERENCE   2  (bases 1 to 10519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04767-1"; journalName: "Nature"; date: 
            "2022-06"; volume: "606"; issue: "7916"; pages: "930-936"
COMMENT     Sequence Label: pLIX401 C-term SETX
FEATURES             Location/Qualifiers
     source          1..10519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2805..2922
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2976..3290
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2984..3002
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3020..3038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3055..3073
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3091..3109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3127..3145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3162..3180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3198..3216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3263..3287
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3311..3335
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6156..6180)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6198..6215
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        6225..6735
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6654..6672
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6745..7341
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6745..6764)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7241..7261
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7342..7395
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7396..8013,8014..8019,8020..8055,8056..8058,8059..8094,
                     8095..8097,8098..8133,8134..8142)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(8182..8202)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8600..8611)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8816..9049
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9121..9242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9158..9177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9212..9231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9282..9417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9417..9488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9489..9557,9558..10349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9707..9726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2821 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2881 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2941 agcagagatc cactttggcc gcgaatcgat atgtcgagtt tactccctat cagtgataga
     3001 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
     3061 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     3121 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
     3181 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
     3241 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagaattg
     3301 gctagcatca acaagtttgt acaaaaaagc aggctccgcg gccgccccct tcaccatggt
     3361 attgaatact tttgaaacag tggcacaaga atggctcaac tctccaaata gagagaattt
     3421 ctatcagttg caagtacgaa aatttcctgc cgattatata aaatactggg agtttgcagt
     3481 ttatctggaa gaatgtgaac tggctaaaca gctttatcca aaggaaaacg atttggtgtt
     3541 tttagctcct gagagaataa atgaagagaa gaaagataca gagagaaatg acatacaaga
     3601 tctccacgaa tatcattctg gttatgttca taaatttcgc cgcacgtcag tcatgcgtaa
     3661 tgggaaaact gagtgttacc tttccatcca gactcaagag aactttccgg ccaatttaaa
     3721 cgaacttgtg aattgtattg taatcagttc tctggtaact acacaaagga agttgaaagc
     3781 catgtctctg ttgggtagtc ggaaccaact ggctagagct gttctgaatc caaaccctat
     3841 ggacttctgt acaaaagatt tactgactac aacatctgag agaattattg cgtacttaag
     3901 agatttcaat gaagatcaaa agaaagcaat agaaactgca tatgctatgg tgaaacactc
     3961 accatcagtt gccaaaatct gcttgattca tggaccacct ggaacaggaa aatcaaaaac
     4021 tattgttggc ctcctctatc gtctactgac agagaaccag aggaaggggc attcagacga
     4081 aaactccaat gccaaaatca aacaaaaccg tgtcctcgtg tgtgcacctt ccaatgcagc
     4141 tgttgatgaa ctcatgaaaa aaattatcct tgaattcaaa gaaaaatgta aagacaagaa
     4201 gaatccttta ggaaactgtg gagatataaa tttagtacga ctgggtccag aaaagtctat
     4261 taatagtgag gttctaaagt tcagtttgga cagccaagta aaccacagaa tgaaaaaaga
     4321 gttaccttct catgttcagg cgatgcataa aagaaaggaa tttctagatt atcagctgga
     4381 tgagctttcc cggcagcgag ctctatgccg aggtggacgg gaaatacaga ggcaagaatt
     4441 agatgaaaac atttccaaag tttctaagga aaggcaggaa cttgcttcta aaattaaaga
     4501 ggttcaagga cgcccacaga aaacacagag tatcatcatc ttagagtccc atatcatctg
     4561 ctgcacgttg agcacaagtg gtggtttact acttgagtct gctttccgtg ggcaaggggg
     4621 tgtccccttc agctgtgtca ttgttgatga ggctggacag tcttgtgaaa ttgagactct
     4681 tactccactc atccatcgct gcaataagct catcctagta ggagatccta agcagctccc
     4741 tccgacagtc atctctatga aagcacagga gtatggctac gaccagtcaa tgatggctcg
     4801 cttctgcaga ctgctggaag agaatgtaga acacaacatg atcagcaggc tgcccattct
     4861 acagctcact gttcagtaca ggatgcatcc agacatatgc ctcttccctt ctaattatgt
     4921 ttataacaga aacttaaaaa caaatagaca gacagaagcc attcgatgtt catcagattg
     4981 gccatttcag ccataccttg tgtttgatgt tggagatggt tcagaaagac gggataatga
     5041 ctcatatata aatgttcaag aaataaaact ggtgatggaa ataattaagc ttattaaaga
     5101 caaaagaaag gatgttagtt ttcgaaacat tggcataata actcattaca aggcccagaa
     5161 gacgatgatt cagaaggatt tggacaaaga gttcgataga aaaggaccag cagaagtaga
     5221 cactgtggat gcattccagg gtcggcagaa ggattgtgtt attgttacgt gtgtcagagc
     5281 aaatagcatc caaggttcaa ttggattcct ggcaagtttg cagagattga atgtcaccat
     5341 cacacgagcc aagtacagcc tcttcatcct cggacatttg aggaccctga tggaaaacca
     5401 gcattggaat cagctgattc aggatgctca gaagcgtggt gccattatta agacctgtga
     5461 caaaaactat agacatgatg cagtgaagat tctgaaactc aagcctgtgc tgcagagaag
     5521 tctcactcac cctcctacca tagccccaga ggggtccaga ccccagggtg gtttgcccag
     5581 cagcaagcta gacagtggat ttgccaagac atctgttgct gcttctctat accacacacc
     5641 ctctgactcc aaggaaatta ctcttactgt tacttcaaag gaccctgaaa gacctcctgt
     5701 tcatgaccaa cttcaggacc cacgactgct gaagaggatg ggcattgagg tcaaaggagg
     5761 aatattcctt tgggatccac aaccctcgag cccccagcat cctggagcaa cacctcctac
     5821 gggcgagccg ggcttccctg tcgttcacca ggacctgagc catatacagc agcccgctgc
     5881 tgtagtggct gctctgagca gccacaaacc tcccgtgcgg ggcgaacctc cagctgccag
     5941 tcccgaggct tccacgtgtc agagcaaatg tgatgacccg gaagaggagc tctgtcacag
     6001 gagagaggcc agggctttca gtgaagggga gcaggagaag tgtggttccg agacccatca
     6061 caccaggagg aactctaggt gggacaagag gacactggag caggaggaca gcagttccaa
     6121 gaaaagaaag cttttataga agggtgggcg cgccgaccca gctttcttgt acaaagtggt
     6181 ttagtaatga accggtccac caccaccacc accactaagg atccggggtt ggggttgcgc
     6241 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     6301 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     6361 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     6421 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     6481 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     6541 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     6601 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     6661 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     6721 gacctctctc cccagcaatt caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     6781 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6841 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6901 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6961 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7021 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7081 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7141 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7201 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7261 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7321 atgacccgca agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa
     7381 gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg agctctggaa
     7441 ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     7501 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     7561 ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg
     7621 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
     7681 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     7741 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     7801 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
     7861 gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca agcaattgag
     7921 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
     7981 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
     8041 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
     8101 gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc
     8161 aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     8221 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     8281 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8341 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8401 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8461 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8521 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8581 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8641 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     8701 ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca
     8761 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8821 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     8881 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8941 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     9001 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     9061 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     9121 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9181 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9241 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     9301 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     9361 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt
     9421 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9481 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9541 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9601 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9661 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9721 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9781 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9841 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9901 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9961 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10021 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10081 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10141 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10201 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10261 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10321 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10381 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10441 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10501 aaaagatcaa aggatcttc
//
