LOCUS       sequence-469970-       12075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ADAR1 p150.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Tang S, Stokasimov E, Cui Y, Pellman D
  TITLE     Breakage of cytoplasmic chromosomes by pathological DNA base 
            excision repair.
  JOURNAL   Nature. 2022 Jun;606(7916):930-936. doi: 10.1038/s41586-022-04767-1.
            Epub 2022 Apr 27.
  PUBMED    35477155
REFERENCE   2  (bases 1 to 12075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04767-1"; journalName: "Nature"; date: 
            "2022-06"; volume: "606"; issue: "7916"; pages: "930-936"
COMMENT     Sequence Label: pHAGE Flag HA ADAR1 p150
FEATURES             Location/Qualifiers
     source          1..12075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1036..1041,1042..1059,1060..1066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1079..1101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1098..1114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1164..1183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3436..3553
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3605..3908
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3909..4112
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4063..4083
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4109..4133
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4209..4218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4218..4241
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4254..4280
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4296..4320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8605..8629)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8642..9209
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8659..9211
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8803..8820)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9030..9049
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9223..9822
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9223..9242)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9719..9739
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9839..10427
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9892..9912)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10310..10321)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10502..10735
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10854..10872)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10940..11044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11045..11113,11114..11905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11263..11282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattaatt cacaaatggc
     3421 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     3481 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     3541 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga ctagtggagt
     3601 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3661 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3721 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3781 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     3841 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     3901 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     3961 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4021 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4081 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     4141 gacgccatcc acgctgtttt gacctccata gaagacaccg gcggccgcag atctctcgag
     4201 gttgatctgc caccatggac tacaaggatg acgatgacaa gctcgatgga ggatacccat
     4261 acgatgttcc agattacgct gctcgaggtt atcaaacaag tttgtacaaa aaagcaggct
     4321 ccaccatgaa tccgcggcag gggtattccc tcagcggata ctacacccat ccatttcaag
     4381 gctatgagca cagacagctc agataccagc agcctgggcc aggatcttcc cccagtagtt
     4441 tcctgcttaa gcaaatagaa tttctcaagg ggcagctccc agaagcaccg gtgattggaa
     4501 agcagacacc gtcactgcca ccttccctcc caggactccg gccaaggttt ccagtactac
     4561 ttgcctccag taccagaggc aggcaagtgg acatcagggg tgtccccagg ggcgtgcatc
     4621 tcggaagtca ggggctccag agagggttcc agcatccttc accacgtggc aggagtctgc
     4681 cacagagagg tgttgattgc ctttcctcac atttccagga actgagtatc taccaagatc
     4741 aggaacaaag gatcttaaag ttcctggaag agcttgggga agggaaggcc accacagcac
     4801 atgatctgtc tgggaaactt gggactccga agaaagaaat caatcgagtt ttatactccc
     4861 tggcaaagaa gggcaagcta cagaaagagg caggaacacc ccctttgtgg aaaatcgcgg
     4921 tctccactca ggcttggaac cagcacagcg gagtggtaag accagacggt catagccaag
     4981 gagccccaaa ctcagacccg agtttggaac cggaagacag aaactccaca tctgtctcag
     5041 aagatcttct tgagcctttt attgcagtct cagctcaggc ttggaaccag cacagcggag
     5101 tggtaagacc agacagtcat agccaaggat ccccaaactc agacccaggt ttggaacctg
     5161 aagacagcaa ctccacatct gccttggaag atcctcttga gtttttagac atggccgaga
     5221 tcaaggagaa aatctgcgac tatctcttca atgtgtctga ctcctctgcc ctgaatttgg
     5281 ctaaaaatat tggccttacc aaggcccgag atataaatgc tgtgctaatt gacatggaaa
     5341 ggcaggggga tgtctataga caagggacaa cccctcccat atggcatttg acagcacatg
     5401 atctgtctgg gaaacttggg actccgaaga aagaaatcaa tcgagtttta tactccctgg
     5461 caaagaaggg caagctacag aaagaggcag gaacaccccc tttgtggaaa atcgcggtct
     5521 ccactcaggc ttggaaccag cacagcggag tggtaagacc agacggtcat agccaaggag
     5581 ccccaaactc agacccgagt ttggaaccgg aagacagaaa ctccacatct gtctcagaag
     5641 atcttcttga gccttttatt gcagtctcag ctcaggcttg gaaccagcac agcggagtgg
     5701 taagaccaga cagtcatagc caaggatccc caaactcaga cccaggtttg gaacctgaag
     5761 acagcaactc cacatctgcc ttggaagatc ctcttgagtt tttagacatg gccgagatca
     5821 aggagaaaat ctgcgactat ctcttcaatg tgtctgactc ctctgccctg aatttggcta
     5881 aaaatattgg ccttaccaag gcccgagata taaatgctgt gctaattgac atggaaaggc
     5941 agggggatgt ctatagacaa gggacaaccc ctcccatatg gcatttgaca gacaagaagc
     6001 gagagaggat gcaaatcaag agaaatacga acagtgttcc tgaaaccgct ccagctgcaa
     6061 tccctgagac caaaagaaac gcagagttcc tcacctgtaa tatacccaca tcaaatgcct
     6121 caaataacat ggtaaccaca gaaaaagtgg agaatgggca ggaacctgtc ataaagttag
     6181 aaaacaggca agaggccaga ccagaaccag caagactgaa accacctgtt cattacaatg
     6241 gcccctcaaa agcagggtat gttgactttg aaaatggcca gtgggccaca gatgacatcc
     6301 cagatgactt gaatagtatc cgcgcagcac caggtgagtt tcgagccatc atggagatgc
     6361 cctccttcta cagtcatggc ttgccacggt gttcacccta caagaaactg acagagtgcc
     6421 agctgaagaa ccccatcagc gggctgttag aatatgccca gttcgctagt caaacctgtg
     6481 agttcaacat gatagagcag agtggaccac cccatgaacc tcgatttaaa ttccaggttg
     6541 tcatcaatgg ccgagagttt cccccagctg aagctggaag caagaaagtg gccaagcagg
     6601 atgcagctat gaaagccatg acaattctgc tagaggaagc caaagccaag gacagtggaa
     6661 aatcagaaga atcatcccac tattccacag agaaagaatc agagaagact gcagagtccc
     6721 agacccccac cccttcagcc acatccttct tttctgggaa gagccccgtc accacactgc
     6781 ttgagtgtat gcacaaattg gggaactcct gtgaattccg tctcctgtcc aaagaaggcc
     6841 ctgcccatga acccaagttc caatactgtg ttgcagtggg agcccaaact ttccccagtg
     6901 tgagtgctcc cagcaagaaa gtggcaaagc agatggccgc agaggaagcc atgaaggccc
     6961 tgcatgggga ggcgaccaac tccatggctt ctgataacca gcctgaaggt atgatctcag
     7021 agtcacttga taacttggaa tccatgatgc ccaacaaggt caggaagatt ggcgagctcg
     7081 tgagatacct gaacaccaac cctgtgggtg gccttttgga gtacgcccgc tcccatggct
     7141 ttgctgctga attcaagttg gtcgaccagt ccggacctcc tcacgagccc aagttcgttt
     7201 accaagcaaa agttgggggt cgctggttcc cagccgtctg cgcacacagc aagaagcaag
     7261 gcaagcagga agcagcagat gcggctctcc gtgtcttgat tggggagaac gagaaggcag
     7321 aacgcatggg tttcacagag gtaaccccag tgacaggggc cagtctcaga agaactatgc
     7381 tcctcctctc aaggtcccca gaagcacagc caaagacact ccctctcact ggcagcacct
     7441 tccatgacca gatagccatg ctgagccacc ggtgcttcaa cactctgact aacagcttcc
     7501 agccctcctt gctcggccgc aagattctgg ccgccatcat tatgaaaaaa gactctgagg
     7561 acatgggtgt cgtcgtcagc ttgggaacag ggaatcgctg tgtaaaagga gattctctca
     7621 gcctaaaagg agaaactgtc aatgactgcc atgcagaaat aatctcccgg agaggcttca
     7681 tcaggtttct ctacagtgag ttaatgaaat acaactccca gactgcgaag gatagtatat
     7741 ttgaacctgc taagggagga gaaaagctcc aaataaaaaa gactgtgtca ttccatctgt
     7801 atatcagcac tgctccgtgt ggagatggcg ccctctttga caagtcctgc agcgaccgtg
     7861 ctatggaaag cacagaatcc cgccactacc ctgtcttcga gaatcccaaa caaggaaagc
     7921 tccgcaccaa ggtggagaac ggagaaggca caatccctgt ggaatccagt gacattgtgc
     7981 ctacgtggga tggcattcgg ctcggggaga gactccgtac catgtcctgt agtgacaaaa
     8041 tcctacgctg gaacgtgctg ggcctgcaag gggcactgtt gacccacttc ctgcagccca
     8101 tttatctcaa atctgtcaca ttgggttacc ttttcagcca agggcatctg acccgtgcta
     8161 tttgctgtcg tgtgacaaga gatgggagtg catttgagga tggactacga catcccttta
     8221 ttgtcaacca ccccaaggtt ggcagagtca gcatatatga ttccaaaagg caatccggga
     8281 agactaagga gacaagcgtc aactggtgtc tggctgatgg ctatgacctg gagatcctgg
     8341 acggtaccag aggcactgtg gatgggccac ggaatgaatt gtcccgggtc tccaaaaaga
     8401 acatttttct tctatttaag aagctctgct ccttccgtta ccgcagggat ctactgagac
     8461 tctcctatgg tgaggccaag aaagctgccc gtgactacga gacggccaag aactacttca
     8521 aaaaaggcct gaaggatatg ggctatggga actggattag caaaccccag gaggaaaaga
     8581 acttttatct ctgcccagta taggacccag ctttcttgta caaagtggtt cgataacgaa
     8641 ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     8701 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     8761 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     8821 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     8881 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     8941 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     9001 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     9061 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     9121 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     9181 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgaccga gtacaagccc
     9241 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     9301 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     9361 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     9421 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     9481 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     9541 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     9601 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     9661 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     9721 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     9781 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gatgcatcga tggatcctaa
     9841 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     9901 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     9961 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
    10021 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
    10081 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
    10141 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
    10201 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
    10261 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
    10321 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
    10381 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
    10441 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
    10501 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
    10561 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    10621 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    10681 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
    10741 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
    10801 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
    10861 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
    10921 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    10981 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    11041 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    11101 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    11161 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    11221 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    11281 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    11341 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    11401 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    11461 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    11521 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    11581 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    11641 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    11701 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    11761 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    11821 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    11881 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    11941 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    12001 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    12061 gatcaaagga tcttc
//
