LOCUS       sequence-469972-        9894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ADAR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Tang S, Stokasimov E, Cui Y, Pellman D
  TITLE     Breakage of cytoplasmic chromosomes by pathological DNA base 
            excision repair.
  JOURNAL   Nature. 2022 Jun;606(7916):930-936. doi: 10.1038/s41586-022-04767-1.
            Epub 2022 Apr 27.
  PUBMED    35477155
REFERENCE   2  (bases 1 to 9894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04767-1"; journalName: "Nature"; date: 
            "2022-06"; volume: "606"; issue: "7916"; pages: "930-936"
COMMENT     Sequence Label: pHAGE Flag HA ADAR2
FEATURES             Location/Qualifiers
     source          1..9894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1036..1041,1042..1059,1060..1066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1079..1101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1098..1114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1164..1183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3436..3553
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3605..3908
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3909..4112
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4063..4083
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4109..4133
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4209..4218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4218..4241
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4254..4280
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4296..4320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6424..6448)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6461..7028
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6478..7030
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6622..6639)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6849..6868
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7042..7641
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7042..7061)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7538..7558
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7658..8246
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7711..7731)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8129..8140)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8321..8554
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8673..8691)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8759..8863
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8864..8932,8933..9724)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9082..9101)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattaatt cacaaatggc
     3421 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     3481 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     3541 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga ctagtggagt
     3601 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3661 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3721 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3781 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     3841 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     3901 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     3961 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4021 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4081 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     4141 gacgccatcc acgctgtttt gacctccata gaagacaccg gcggccgcag atctctcgag
     4201 gttgatctgc caccatggac tacaaggatg acgatgacaa gctcgatgga ggatacccat
     4261 acgatgttcc agattacgct gctcgaggtt atcaaacaag tttgtacaaa aaagttggca
     4321 tggatataga agatgaagaa aacatgagtt ccagcagcac tgatgtgaag gaaaaccgca
     4381 atctggacaa cgtgtccccc aaggatggca gcacacctgg gcctggcgag ggctctcagc
     4441 tctccaatgg gggtggtggt ggccccggca gaaagcggcc cctggaggag ggcagcaatg
     4501 gccactccaa gtaccgcctg aagaaaagga ggaaaacacc agggcccgtc ctccccaaga
     4561 acgccctgat gcagctgaat gagatcaagc ctggtttgca gtacacactc ctgtcccaga
     4621 ctgggcccgt gcacgcgcct ttgtttgtca tgtctgtgga ggtgaatggc caggtttttg
     4681 agggctctgg tcccacaaag aaaaaggcaa aactccatgc tgctgagaag gccttgaggt
     4741 ctttcgttca gtttcctaat gcctctgagg cccacctggc catggggagg accctgtctg
     4801 tcaacacgga cttcacatct gaccaggccg acttccctga cacgctcttc aatggttttg
     4861 aaactcctga caaggcggag cctccctttt acgtgggctc caatggggat gactccttca
     4921 gttccagcgg ggacctcagc ttgtctgctt ccccggtgcc tgccagccta gcccagcctc
     4981 ctctccctgc cttaccacca ttcccacccc cgagtgggaa gaatcccgtg atgatcttga
     5041 acgaactgcg cccaggactc aagtatgact tcctctccga gagcggggag agccatgcca
     5101 agagcttcgt catgtctgtg gtcgtggatg gtcagttctt tgaaggctcg gggagaaaca
     5161 agaagcttgc caaggcccgg gctgcgcagt ctgccctggc cgccattttt aacttgcact
     5221 tggatcagac gccatctcgc cagcctattc ccagtgaggg tcttcagctg catttaccgc
     5281 aggttttagc tgacgctgtc tcacgcctgg tcctgggtaa gtttggtgac ctgaccgaca
     5341 acttctcctc ccctcacgct cgcagaaaag tgctggctgg agtcgtcatg acaacaggca
     5401 cagatgttaa agatgccaag gtgataagtg tttctacagg aacaaaatgt attaatggtg
     5461 aatacatgag tgatcgtggc cttgcattaa atgactgcca tgcagaaata atatctcgga
     5521 gatccttgct cagatttctt tatacacaac ttgagcttta cttaaataac aaagatgatc
     5581 aaaaaagatc catctttcag aaatcagagc gaggggggtt taggctgaag gagaatgtcc
     5641 agtttcatct gtacatcagc acctctccct gtggagatgc cagaatcttc tcaccacatg
     5701 agccaatcct ggaagaacca gcagatagac acccaaatcg taaagcaaga ggacagctac
     5761 ggaccaaaat agagtctggt gaggggacga ttccagtgcg ctccaatgcg agcatccaaa
     5821 cgtgggacgg ggtgctgcaa ggggagcggc tgctcaccat gtcctgcagt gacaagattg
     5881 cacgctggaa cgtggtgggc atccagggat ccctgctcag cattttcgtg gagcccattt
     5941 acttctcgag catcatcctg ggcagccttt accacgggga ccacctttcc agggccatgt
     6001 accagcggat ctccaacata gaggacctgc cacctctcta caccctcaac aagcctttgc
     6061 tcagtggcat cagcaatgca gaagcacggc agccagggaa ggcccccaac ttcagtgtca
     6121 actggacggt aggcgactcc gctattgagg tcatcaacgc cacgactggg aaggatgagc
     6181 tgggccgcgc gtcccgcctg tgtaagcacg cgttgtactg tcgctggatg cgtgtgcacg
     6241 gcaaggttcc ctcccactta ctacgctcca agattaccaa acccaacgtg taccatgagt
     6301 ccaagctggc ggcaaaggag taccaggccg ccaaggcgcg tctgttcaca gccttcatca
     6361 aggcggggct gggggcctgg gtggagaagc ccaccgagca ggaccagttc tcactcacgc
     6421 cctgcccaac tttcttgtac aaagtggttc gataacgaat tccgcccccc ccccctaacg
     6481 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     6541 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     6601 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     6661 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     6721 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     6781 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     6841 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     6901 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     6961 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     7021 gatgataata tggccacaac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     7081 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7141 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     7201 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7261 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7321 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7381 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7441 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7501 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7561 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7621 acccgcaagc ccggtgcctg atgcatcgat ggatcctaat caacctctgg attacaaaat
     7681 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7741 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7801 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7861 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7921 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7981 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8041 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     8101 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8161 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8221 gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca
     8281 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     8341 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     8401 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8461 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8521 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     8581 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag
     8641 gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     8701 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     8761 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8821 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8881 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8941 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9001 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9061 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     9121 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9181 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9241 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9301 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9361 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9421 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9481 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9541 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9601 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9661 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9721 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9781 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9841 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
