LOCUS       sequence-469980-       10011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-MAP4K3
FEATURES             Location/Qualifiers
     source          1..10011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2179..2205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4843..5417,5418..5420,5421..5429)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5010..5027)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5237..5256
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    5868..6456
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5921..5941)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6339..6350)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6528..6761
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6839..6960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6876..6895)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6930..6949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7000..7135
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7155..7174)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7156..7174)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7184..7201)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7184..7200)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7193..7215)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7342..7797
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7429..7448)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7639..7660
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7823..7927
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7928..7996,7997..8788)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8146..8165)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8959..9547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9448..9467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9701..9718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9835..9856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9871..9876,9877..9894,9895..9901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9909..9925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9914..9936
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9933..9949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9933..9949
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9968..9988
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9970..9988
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccttacgac
     2161 gttcctgatt acgcgggata tccctacgac gtgccagatt acgcgggtag tggtaacccc
     2221 ggcttcgatt tgtcccgccg gaacccgcag gaggacttcg agctgattca gcgcatcggc
     2281 agcggcacct acggcgacgt ctacaaggca cggaatgtta acactggtga attagcagca
     2341 attaaagtaa taaaattgga accaggagaa gactttgcag ttgtgcagca agaaattatc
     2401 atgatgaaag actgtaaaca cccaaatatt gttgcttatt ttggaagcta tctcaggcga
     2461 gataagcttt ggatttgcat ggagttttgt ggaggtggtt ctttacagga tatttatcac
     2521 gtaactggac ctctgtcaga actgcaaatt gcatatgtta gcagagaaac actgcaggga
     2581 ttatattatc ttcacagtaa aggaaaaatg cacagagata taaagggagc taacattcta
     2641 ttaacggata atggtcatgt gaaattggct gattttggag tatctgcaca gataacagct
     2701 acaattgcca aacggaagtc tttcattggc acaccatatt ggatggctcc agaagttgca
     2761 gctgttgaga ggaagggggg ttacaatcaa ctctgtgatc tctgggcagt gggaatcact
     2821 gccatagaac ttgcagagct tcagcctcct atgtttgact tacacccaat gagagcatta
     2881 tttctaatga caaaaagcaa ttttcagcct cctaaactaa aggataaaat gaaatggtca
     2941 aatagttttc atcactttgt gaaaatggca cttaccaaaa atccgaaaaa aagacctact
     3001 gctgaaaaat tattacagca tccttttgta acacaacatt tgacacggtc tttggcaatc
     3061 gagctgttgg ataaagtaaa taatccagat cattccactt accatgattt cgatgatgat
     3121 gatcctgagc ctcttgttgc tgtaccacat agaattcact caacaagtag aaacgtgaga
     3181 gaagaaaaaa cacgctcaga gataaccttt ggccaagtga aatttgatcc acccttaaga
     3241 aaggagacag aaccacatca tgaacttgat ctgcaactgg aatatggaca aggacaccaa
     3301 ggtggttact ttttaggtgc aaacaagagt cttctcaagt ctgttgaaga agaattgcat
     3361 cagcgaggac acgtcgcaca tttagaagat gatgaaggag atgatgatga atctaaacac
     3421 tcaactctga aagcaaaaat tccacctcct ttgccaccaa agcctaagtc tatcttcata
     3481 ccacaggaaa tgcattctac tgaggatgaa aatcaaggaa caatcaagag atgtcccatg
     3541 tcagggagcc cagcaaagcc atcccaagtt ccacctagac caccacctcc cagattaccc
     3601 ccacacaaac ctgttgcctt aggaaatgga atgagctcct tccagttaaa tggtgaacga
     3661 gatggctcat tatgtcaaca acagaatgaa catagaggca caaacctttc aagaaaagaa
     3721 aagaaagatg taccaaagcc tattagtaat ggtcttcctc caacacctaa agtgcatatg
     3781 ggtgcatgtt tttcaaaagt ttttaatggg tgtcccttga aaattcactg tgcatcatca
     3841 tggataaacc cagatactag agatcagtac ttgatatttg gtgccgaaga agggatttat
     3901 accctcaatc ttaatgaact tcatgaaaca tcaatggaac agctattccc tcgaaggtgt
     3961 acatggttgt atgtaatgaa caattgcttg ctatcaatat ctggtaaagc ttctcagctt
     4021 tattcccata atttaccagg gctttttgat tatgcaagac aaatgcaaaa gttacctgtt
     4081 gctattccag cacacaaact ccctgacaga atactgccaa ggaaattttc tgtatcagca
     4141 aaaatccctg aaaccaaatg gtgccagaag tgttgtgttg taagaaatcc ttacacgggc
     4201 cataaatacc tatgtggagc acttcagact agcattgttc tattagaatg ggttgaacca
     4261 atgcagaaat ttatgttaat taagcacata gattttccta taccatgtcc acttagaatg
     4321 tttgaaatgc tggtagttcc tgaacaggag taccctttag tttgtgttgg tgtcagtaga
     4381 ggtagagact tcaaccaagt ggttcgattt gagacggtca atccaaattc tacctcttca
     4441 tggtttacag aatcagatac cccacagaca aatgttactc atgtaaccca actggagaga
     4501 gataccatcc ttgtatgctt ggactgttgt ataaaaatag taaatctcca aggaagatta
     4561 aaatctagca ggaaattgtc atcagaactc acctttgatt tccagattga atcaatagtg
     4621 tgcctacaag acagtgtgct agctttctgg aaacatggaa tgcaaggtag aagttttaga
     4681 tctaatgagg taacacaaga aatttcagat agcacaagaa ttttcaggct gcttggatct
     4741 gacagggtcg tggttttgga aagtaggcca actgataacc ccacagcaaa tagcaatttg
     4801 tacatcctgg cgggtcatga aaacagttac tgagcaattc cgcccctctc cctccccccc
     4861 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     4921 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     4981 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     5041 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     5101 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     5161 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     5221 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     5281 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     5341 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     5401 aaaaacacga tgataatatg gccacaacca tgaagacctt caacatctct cagcaggacc
     5461 ttgagctggt ggaagttgcc actgagaaaa tcaccatgct ctatgaggac aacaagcacc
     5521 atgttggtgc tgccatcagg accaagacag gagaaatcat ctctgctgtc cacattgaag
     5581 cctacattgg cagggtcact gtgtgtgcag aggccattgc cattgggtct gcagtctcca
     5641 atgggcagaa ggactttgac accattgtgg ctgtgaggca cccctacagt gatgaggtgg
     5701 acaggtccat cagagtggtg tccccctgtg gcatgtgcag ggagctcatc tcagactatg
     5761 cccctgattg ctttgttctg attgagatga atggcaagct ggtcaagaca accattgagg
     5821 agctgattcc actgaaatac accagaaact aaacgcgtta agtcgacaat caacctctgg
     5881 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5941 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6001 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6061 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6121 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6181 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6241 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6301 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6361 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6421 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     6481 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6541 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6601 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6661 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6721 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     6781 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     6841 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6901 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6961 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     7021 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7081 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     7141 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     7201 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7261 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7321 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7381 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7441 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7501 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7561 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7621 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7681 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7741 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     7801 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7861 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7921 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7981 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8041 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8101 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8161 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8221 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8281 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8341 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8401 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8461 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8521 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8581 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8641 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8701 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8761 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8821 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8881 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8941 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9001 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9061 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     9661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     9961 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag c
//
