LOCUS       sequence-469982-       16679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EcoHIV-mScarlet is a modification of pNL4-3-EcoHIV (Potash et al, 
            PNAS 2005). EcoHIV is rodent-infectable modification of HIV where 
            HIV env replaced for MLV-env.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16679)
  AUTHORS   Li H, Walker M, Ji H, Sikirzhytskaya A, Aksenova M, Shtutman M, 
            Sikirzhytski V, Mactutus CF, Booze RM
  TITLE     Identification of EcoHIV-Infected Cells in Microglia-Manipulated 
            Transgenic Mice.
  JOURNAL   J Vis Exp. 2024 Dec 20;(214). doi: 10.3791/67150.
  PUBMED    39760359
REFERENCE   2  (bases 1 to 16679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2024-12-20"; volume: "(214). doi"; pages: " 
            10.3791/67150"
COMMENT     Sequence Label: EcoHIV-mScarlet
FEATURES             Location/Qualifiers
     source          1..16679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(627..644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(798..1386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(878..897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1557..2348,2349..2417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2180..2199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2418..2522)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2590..2608
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2646..2668)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2768..2787
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             4480..4737
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             4893..6395
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             6188..9199
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    8884..9001
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    12417..12650
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12835..12879
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             13173..13199
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             13445..14140
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(13578..13596)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     13846..13865
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    14169..14742
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    14192..14744
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(14336..14353)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     14563..14582
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             15033..15666
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tatttttgag acagggtctc actctgtcac ctaggctgga gtgcagtggt gcaatcacag
       61 ctcactgcag cctcaacgtc gtaagctcaa gcgatcctcc cacctcagcc tgcctggtag
      121 ctgagactac aagcgacgcc ccagttaatt tttgtatttt tggtagaggc agcgttttgc
      181 cgtgtggccc tggctggtct cgaactcctg ggctcaagtg atccagcctc agcctcccaa
      241 agtgctggga caaccggggc cagtcactgc acctggccct aaaccataat ttctaatctt
      301 ttggctaatt tgttagtcct acaaaggcag tctagtcccc aggcaaaaag ggggtttgtt
      361 tcgggaaagg gctgttactg tctttgtttc aaactataaa ctaagttcct cctaaactta
      421 gttcggccta cacccaggaa tgaacaagga gagcttggag gttagaagca cgatggaatt
      481 ggttaggtca gatctctttc actgtctgag ttataatttt gcaatggtgg ttcaaagact
      541 gcccgcttct gacaccagtc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      601 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      781 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      841 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      901 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      961 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1021 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1081 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1141 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1201 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1261 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1321 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1381 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1441 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1501 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1561 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1621 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1681 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1741 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1801 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1861 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1921 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1981 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2041 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2101 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2161 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2221 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2281 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2341 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2401 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2461 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2521 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2581 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     2641 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     2701 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     2761 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     2821 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     2881 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccaggggag gcagagattg
     2941 cagtaagctg agatcgcagc actgcactcc agcctgggcg acagagtaag actctgtctc
     3001 aaaaataaaa taaataaatc aatcagatat tccaatcttt tcctttattt atttatttat
     3061 tttctatttt ggaaacacag tccttcctta ttccagaatt acacatatat tctatttttc
     3121 tttatatgct ccagtttttt ttagaccttc acctgaaatg tgtgtataca aaatctaggc
     3181 cagtccagca gagcctaaag gtaaaaaata aaataataaa aaataaataa aatctagctc
     3241 actccttcac atcaaaatgg agatacagct gttagcatta aataccaaat aacccatctt
     3301 gtcctcaata attttaagcg cctctctcca ccacatctaa ctcctgtcaa aggcatgtgc
     3361 cccttccggg cgctctgctg tgctgccaac caactggcat gtggactctg cagggtccct
     3421 aactgccaag ccccacagtg tgccctgagg ctgccccttc cttctagcgg ctgcccccac
     3481 tcggctttgc tttccctagt ttcagttact tgcgttcagc caaggtctga aactaggtgc
     3541 gcacagagcg gtaagactgc gagagaaaga gaccagcttt acagggggtt tatcacagtg
     3601 caccctgaca gtcgtcagcc tcacaggggg tttatcacat tgcaccctga cagtcgtcag
     3661 cctcacaggg ggtttatcac agtgcaccct tacaatcatt ccatttgatt cacaattttt
     3721 ttagtctcta ctgtgcctaa cttgtaagtt aaatttgatc agaggtgtgt tcccagaggg
     3781 gaaaacagta tatacagggt tcagtactat cgcatttcag gcctccacct gggtcttgga
     3841 atgtgtcccc cgaggggtga tgactacctc agttggatct ccacaggtca cagtgacaca
     3901 agataaccaa gacacctccc aaggctacca caatgggccg ccctccacgt gcacatggcc
     3961 ggaggaactg ccatgtcgga ggtgcaagca cacctgcgca tcagagtcct tggtgtggag
     4021 ggagggacca gcgcagcttc cagccatcca cctgatgaac agaacctagg gaaagcccca
     4081 gttctactta caccaggaaa ggctggaagg gctaatttgg tcccaaaaaa gacaagagat
     4141 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     4201 agggccaggg atcagatatc cactgacctt tggatggtgc ttcaagttag taccagttga
     4261 accagagcaa gtagaagagg ccaatgaagg agagaacaac agcttgttac accctatgag
     4321 ccagcatggg atggaggacc cggagggaga agtattagtg tggaagtttg acagcctcct
     4381 agcatttcgt cacatggccc gagagctgca tccggagtac tacaaagact gctgacatcg
     4441 agctttctac aagggacttt ccgctgggga ctttccaggg aggtgtggcc tgggcgggac
     4501 tggggagtgg cgagccctca gatgctacat ataagcagct gctttttgcc tgtactgggt
     4561 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4621 ttaagcctca ataaagcttg ccttgagtgc tcaaagtagt gtgtgcccgt ctgttgtgtg
     4681 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     4741 gcgcccgaac agggacttga aagcgaaagt aaagccagag gagatctctc gacgcaggac
     4801 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     4861 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtcg gtattaagcg
     4921 ggggagaatt agataaatgg gaaaaaattc ggttaaggcc agggggaaag aaacaatata
     4981 aactaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     5041 ttttagagac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     5101 caggatcaga agaacttaga tcattatata atacaatagc agtcctctat tgtgtgcatc
     5161 aaaggataga tgtaaaagac accaaggaag ccttagataa gatagaggaa gagcaaaaca
     5221 aaagtaagaa aaaggcacag caagcagcag ctgacacagg aaacaacagc caggtcagcc
     5281 aaaattaccc tatagtgcag aacctccagg ggcaaatggt acatcaggcc atatcaccta
     5341 gaactttaaa tgcatgggta aaagtagtag aagagaaggc tttcagccca gaagtaatac
     5401 ccatgttttc agcattatca gaaggagcca ccccacaaga tttaaatacc atgctaaaca
     5461 cagtgggggg acatcaagca gccatgcaaa tgttaaaaga gaccatcaat gaggaagctg
     5521 cagaatggga tagattgcat ccagtgcatg cagggcctat tgcaccaggc cagatgagag
     5581 aaccaagggg aagtgacata gcaggaacta ctagtaccct tcaggaacaa ataggatgga
     5641 tgacacataa tccacctatc ccagtaggag aaatctataa aagatggata atcctgggat
     5701 taaataaaat agtaagaatg tatagcccta ccagcattct ggacataaga caaggaccaa
     5761 aggaaccctt tagagactat gtagaccgat tctataaaac tctaagagcc gagcaagctt
     5821 cacaagaggt aaaaaattgg atgacagaaa ccttgttggt ccaaaatgcg aacccagatt
     5881 gtaagactat tttaaaagca ttgggaccag gagcgacact agaagaaatg atgacagcat
     5941 gtcagggagt ggggggaccc ggccataaag caagagtttt ggctgaagca atgagccaag
     6001 taacaaatcc agctaccata atgatacaga aaggcaattt taggaaccaa agaaagactg
     6061 ttaagtgttt caattgtggc aaagaagggc acatagccaa aaattgcagg gcccctagga
     6121 aaaagggctg ttggaaatgt ggaaaggaag gacaccaaat gaaagattgt actgagagac
     6181 aggctaattt tttagggaag atctggcctt cccacaaggg aaggccaggg aattttcttc
     6241 agagcagacc agagccaaca gccccaccag aagagagctt caggtttggg gaagagacaa
     6301 caactccctc tcagaagcag gagccgatag acaaggaact gtatccttta gcttccctca
     6361 gatcactctt tggcagcgac ccctcgtcac aataaagata ggggggcaat taaaggaagc
     6421 tctattagat acaggagcag atgatacagt attagaagaa atgaatttgc caggaagatg
     6481 gaaaccaaaa atgatagggg gaattggagg ttttatcaaa gtaagacagt atgatcagat
     6541 actcatagaa atctgcggac ataaagctat aggtacagta ttagtaggac ctacacctgt
     6601 caacataatt ggaagaaatc tgttgactca gattggctgc actttaaatt ttcccattag
     6661 tcctattgag actgtaccag taaaattaaa gccaggaatg gatggcccaa aagttaaaca
     6721 atggccattg acagaagaaa aaataaaagc attagtagaa atttgtacag aaatggaaaa
     6781 ggaaggaaaa atttcaaaaa ttgggcctga aaatccatac aatactccag tatttgccat
     6841 aaagaaaaaa gacagtacta aatggagaaa attagtagat ttcagagaac ttaataagag
     6901 aactcaagat ttctgggaag ttcaattagg aataccacat cctgcagggt taaaacagaa
     6961 aaaatcagta acagtactgg atgtgggcga tgcatatttt tcagttccct tagataaaga
     7021 cttcaggaag tatactgcat ttaccatacc tagtataaac aatgagacac cagggattag
     7081 atatcagtac aatgtgcttc cacagggatg gaaaggatca ccagcaatat tccagtgtag
     7141 catgacaaaa atcttagagc cttttagaaa acaaaatcca gacatagtca tctatcaata
     7201 catggatgat ttgtatgtag gatctgactt agaaataggg cagcatagaa caaaaataga
     7261 ggaactgaga caacatctgt tgaggtgggg atttaccaca ccagacaaaa aacatcagaa
     7321 agaacctcca ttcctttgga tgggttatga actccatcct gataaatgga cagtacagcc
     7381 tatagtgctg ccagaaaagg acagctggac tgtcaatgac atacagaaat tagtgggaaa
     7441 attgaattgg gcaagtcaga tttatgcagg gattaaagta aggcaattat gtaaacttct
     7501 taggggaacc aaagcactaa cagaagtagt accactaaca gaagaagcag agctagaact
     7561 ggcagaaaac agggagattc taaaagaacc ggtacatgga gtgtattatg acccatcaaa
     7621 agacttaata gcagaaatac agaagcaggg gcaaggccaa tggacatatc aaatttatca
     7681 agagccattt aaaaatctga aaacaggaaa gtatgcaaga atgaagggtg cccacactaa
     7741 tgatgtgaaa caattaacag aggcagtaca aaaaatagcc acagaaagca tagtaatatg
     7801 gggaaagact cctaaattta aattacccat acaaaaggaa acatgggaag catggtggac
     7861 agagtattgg caagccacct ggattcctga gtgggagttt gtcaataccc ctcccttagt
     7921 gaagttatgg taccagttag agaaagaacc cataatagga gcagaaactt tctatgtaga
     7981 tggggcagcc aatagggaaa ctaaattagg aaaagcagga tatgtaactg acagaggaag
     8041 acaaaaagtt gtccccctaa cggacacaac aaatcagaag actgagttac aagcaattca
     8101 tctagctttg caggattcgg gattagaagt aaacatagtg acagactcac aatatgcatt
     8161 gggaatcatt caagcacaac cagataagag tgaatcagag ttagtcagtc aaataataga
     8221 gcagttaata aaaaaggaaa aagtctacct ggcatgggta ccagcacaca aaggaattgg
     8281 aggaaatgaa caagtagata aattggtcag tgctggaatc aggaaagtac tatttttaga
     8341 tggaatagat aaggcccaag aagaacatga gaaatatcac agtaattgga gagcaatggc
     8401 tagtgatttt aacctaccac ctgtagtagc aaaagaaata gtagccagct gtgataaatg
     8461 tcagctaaaa ggggaagcca tgcatggaca agtagactgt agcccaggaa tatggcagct
     8521 agattgtaca catttagaag gaaaagttat cttggtagca gttcatgtag ccagtggata
     8581 tatagaagca gaagtaattc cagcagagac agggcaagaa acagcatact tcctcttaaa
     8641 attagcagga agatggccag taaaaacagt acatacagac aatggcagca atttcaccag
     8701 tactacagtt aaggccgcct gttggtgggc ggggatcaag caggaatttg gcattcccta
     8761 caatccccaa agtcaaggag taatagaatc tatgaataaa gaattaaaga aaattatagg
     8821 acaggtaaga gatcaggctg aacatcttaa gacagcagta caaatggcag tattcatcca
     8881 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     8941 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     9001 tcgggtttat tacagggaca gcagagatcc agtttggaaa ggaccagcaa agctcctctg
     9061 gaaaggtgaa ggggcagtag taatacaaga taatagtgac ataaaagtag tgccaagaag
     9121 aaaagcaaag atcatcaggg attatggaaa acagatggca ggtgatgatt gtgtggcaag
     9181 tagacaggat gaggattaac acatggaaaa gattagtaaa acaccatatg tatatttcaa
     9241 ggaaagctaa ggactggttt tatagacatc actatgaaag tactaatcca aaaataagtt
     9301 cagaagtaca catcccacta ggggatgcta aattagtaat aacaacatat tggggtctgc
     9361 atacaggaga aagagactgg catttgggtc agggagtctc catagaatgg aggaaaaaga
     9421 gatatagcac acaagtagac cctgacctag cagaccaact aattcatctg cactattttg
     9481 attgtttttc agaatctgct ataagaaata ccatattagg acgtatagtt agtcctaggt
     9541 gtgaatatca agcaggacat aacaaggtag gatctctaca gtacttggca ctagcagcat
     9601 taataaaacc aaaacagata aagccacctt tgcctagtgt taggaaactg acagaggaca
     9661 gatggaacaa gccccagaag accaagggcc acagagggag ccatacaatg aatggacact
     9721 agagctttta gaggaactta agagtgaagc tgttagacat tttcctagga tatggctcca
     9781 taacttagga caacatatct atgaaactta cggggatact tgggcaggag tggaagccat
     9841 aataagaatt ctgcaacaac tgctgtttat ccatttcaga attgggtgtc gacatagcag
     9901 aataggcgtt actcgacaga ggagagcaag aaatggagcc agtagatcct agactagagc
     9961 cctggaagca tccaggaagt cagcctaaaa ctgcttgtac caattgctat tgtaaaaagt
    10021 gttgctttca ttgccaagtt tgtttcatga caaaagcctt aggcatctcc tatggcagga
    10081 agaagcggag acagcgacga agagctcatc agaacagtca gactcatcaa gcttctctat
    10141 caaagcagta agtagtacat gtaatgcaac ctataatagt agcaatagta gcattagtag
    10201 tagcaataat aatagcaata gttgtgtggt ccatagtaat catagaatat aggaaaatat
    10261 taagacaaag aaaaatagac aggttaattg atagactaat agaaagagca gaagacagtg
    10321 gcaatgagag tgaaggagaa gtatcagcac ttgtggagat gggggtggaa atggggcacc
    10381 atgctccttg ggatattgat gatctgtagt gctgcgcgtt caacgctctc aaaacccctt
    10441 aaaaataagg ttaacccgcg aggcccccta atccccttaa ttcttctgat gctcagaggg
    10501 gtcagtactg cttcgcccgg ctccagtcct catcaagtct ataatatcac ctgggaggta
    10561 accaatggag atcgggagac ggtatgggca acttctggca accaccctct gtggacctgg
    10621 tggcctgacc ttaccccaga tttatgtatg ttagcccacc atggaccatc ttattggggg
    10681 ctagaatatc aatccccttt ttcttctccc ccggggcccc cttgttgctc agggggcagc
    10741 agcccaggct gttccagaga ctgcgaagaa cctttaacct ccctcacccc tcggtgcaac
    10801 actgcctgga acagactcaa gctagaccag acaactcata aatcaaatga gggattttat
    10861 gtttgccccg ggccccaccg cccccgagaa tccaagtcat gtgggggtcc agactccttc
    10921 tactgtgcct attggggctg tgagacaacc ggtagagctt actggaagcc ctcctcatca
    10981 tgggatttca tcacagtaaa caacaatctc acctctgacc aggctgtcca ggtatgcaaa
    11041 gataataagt ggtgcaaccc cttagttatt cggtttacag acgccgggag acgggttact
    11101 tcctggacca caggacatta ctggggctta cgtttgtatg tctccggaca agatccaggg
    11161 cttacatttg ggatccgact cagataccaa aatctaggac cccgcgtccc aatagggcca
    11221 aaccccgttc tggcagacca acagccactc tccaagccca aacctgttaa gtcgccttca
    11281 gtcaccaaac cacccagtgg gactcctctc tcccctaccc aacttccacc ggcgggaacg
    11341 gaaaataggc tgctaaactt agtagacgga gcctaccaag ccctcaacct caccagtcct
    11401 gacaaaaccc aagagtgctg gttgtgtcta gtagcgggac ccccctacta cgaaggggtt
    11461 gccgtcctgg gtacctactc caaccatacc tctgctccag ccaactgctc cgtggcctcc
    11521 caacacaagt tgaccctgtc cgaagtgacc ggacagggac tctgcatagg agcagttccc
    11581 aaaacacatc aggccctatg taataccacc cagacaagca gtcgagggtc ctattatcta
    11641 gttgccccta caggtaccat gtgggcttgt agtaccgggc ttactccatg catctccacc
    11701 accatactga accttaccac tgattattgt gttcttgtcg aactctggcc aagagtcacc
    11761 tatcattccc ccagctatgt ttacggcctg tttgagagat ccaaccgaca caaaagagaa
    11821 ccggtgtcgt taaccctggc cctattattg ggtggactaa ccatgggggg aattgccgct
    11881 ggaataggaa cagggactac tgctctaatg gccactcagc aattccagca gctccaagcc
    11941 gcagtacagg atgatctcag ggaggttgaa aaatcaatct ctaacctaga aaagtctctc
    12001 acttccctgt ctgaagttgt cctacagaat cgaaggggcc tagacttgtt atttctaaaa
    12061 gaaggagggc tgtgtgctgc tctaaaagaa gaatgttgct tctatgcgga ccacacagga
    12121 ctagtgagag acagcatggc caaattgaga gagaggctta atcagagaca gaaactgttt
    12181 gagtcaactc aaggatggtt tgagggactg tttaacagat ccccttggtt taccaccttg
    12241 atatctacca ttatgggacc cctcattgta ctcctaatga ttttgctctt cggaccctac
    12301 attcttaatc gattagtcca atttgttaaa gacaggatat cagtggtcca ggctctagtt
    12361 ttgactcaac aatatcacca gctgaagcct atagagtacg agccataggt gggaatagga
    12421 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    12481 ctgacggtac aggccagaca attattgtct gatatagtgc agcagcagaa caatttgctg
    12541 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caaacagctc
    12601 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    12661 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    12721 aaatctctgg aacagatttg gaataacatg acctggatgg agtgggacag agaaattaac
    12781 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    12841 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    12901 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    12961 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    13021 tttcagaccc acctcccaat cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    13081 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcctt agcacttatc
    13141 tgggacgatc tgcggagcct gtgcctcttc agctaccacc gcttgagaga cttactcttg
    13201 attgtaacga ggattgtgga acttctggga cgcagggggt gggaagccct caaatattgg
    13261 tggaatctcc tacagtattg gagtcaggaa ctaaagaata gtgctgttaa cttgctcaat
    13321 gccacagcca tagcagtagc tgaggggaca gatagggtta tagaagtatt acaagcagct
    13381 tatagagcta ttcgccacat acctagaaga ataagacagg gcttggaaag gattttgcta
    13441 taagatggtg agcaagggcg aggcagtgat caaggagttc atgcggttca aggtgcacat
    13501 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
    13561 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttctcctg
    13621 ggacatcctg tcccctcagt tcatgtacgg ctccagggcc ttcaccaagc accccgccga
    13681 catccccgac tactataagc agtccttccc cgagggcttc aagtgggagc gcgtgatgaa
    13741 cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc tccctggagg acggcaccct
    13801 gatctacaag gtgaagctcc gcggcaccaa cttccctcct gacggccccg taatgcagaa
    13861 gaagacaatg ggctgggaag cgtccaccga gcggttgtac cccgaggacg gcgtgctgaa
    13921 gggcgacatt aagatggccc tgcgcctgaa ggacggcggc cggtacctgg cggacttcaa
    13981 gaccacctac aaggccaaga agcccgtgca gatgcccggc gcctacaacg tcgaccgcaa
    14041 gttggacatc acctcccaca acgaggacta caccgtggtg gaacagtacg aacgctccga
    14101 gggccgccac tccaccggcg gcatggacga gctgtacaag taaagcggcc gcactagtga
    14161 taattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
    14221 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
    14281 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
    14341 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
    14401 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
    14461 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
    14521 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
    14581 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
    14641 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
    14701 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatgggtg gcaagtggtc
    14761 aaaaagtagt gtgattggat ggcctgctgt aagggaaaga atgagacgag ctgagccagc
    14821 agcagatggg gtgggagcag tatctcgaga cctagaaaaa catggagcaa tcacaagtag
    14881 caatacagca gctaacaatg ctgcttgtgc ctggctagaa gcacaagagg aggaagaggt
    14941 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
    15001 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaaagaag
    15061 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
    15121 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
    15181 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
    15241 ccctgtgagc ctgcatggaa tggatgaccc tgagagagaa gtgttagagt ggaggtttga
    15301 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
    15361 ctgacatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
    15421 gggcgggact ggggagtggc gagccctcag atgctgcata taagcagctg ctttttgcct
    15481 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    15541 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    15601 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    15661 ctagcaccca ggaggtagag gttgcagtga gccaagatcg cgccactgca ttccagcctg
    15721 ggcaagaaaa caagactgtc taaaataata ataataagtt aagggtatta aatatattta
    15781 tacatggagg tcataaaaat atatatattt gggctgggcg cagtggctca cacctgcgcc
    15841 cggccctttg ggaggccgag gcaggtggat cacctgagtt tgggagttcc agaccagcct
    15901 gaccaacatg gagaaacccc ttctctgtgt atttttagta gattttattt tatgtgtatt
    15961 ttattcacag gtatttctgg aaaactgaaa ctgtttttcc tctactctga taccacaaga
    16021 atcatcagca cagaggaaga cttctgtgat caaatgtggt gggagaggga ggttttcacc
    16081 agcacatgag cagtcagttc tgccgcagac tcggcgggtg tccttcggtt cagttccaac
    16141 accgcctgcc tggagagagg tcagaccaca gggtgagggc tcagtcccca agacataaac
    16201 acccaagaca taaacaccca acaggtccac cccgcctgct gcccaggcag agccgattca
    16261 ccaagacggg aattaggata gagaaagagt aagtcacaca gagccggctg tgcgggagaa
    16321 cggagttcta ttatgactca aatcagtctc cccaagcatt cggggatcag agtttttaag
    16381 gataacttag tgtgtagggg gccagtgagt tggagatgaa agcgtaggga gtcgaaggtg
    16441 tccttttgcg ccgagtcagt tcctgggtgg gggccacaag atcggatgag ccagtttatc
    16501 aatccggggg tgccagctga tccatggagt gcagggtctg caaaatatct caagcactga
    16561 ttgatcttag gttttacaat agtgatgtta ccccaggaac aatttgggga aggtcagaat
    16621 cttgtagcct gtagctgcat gactcctaaa ccataatttc ttttttgttt ttttttttt
//
