LOCUS       sequence-469986-       11274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 11274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-MAP4K6
FEATURES             Location/Qualifiers
     source          1..11274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2179..2205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(6106..6680,6681..6683,6684..6692)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6273..6290)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6500..6519
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    7131..7719
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7184..7204)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7602..7613)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7791..8024
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8102..8223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8139..8158)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8193..8212
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8263..8398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8418..8437)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8419..8437)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8447..8464)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8447..8463)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8456..8478)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8605..9060
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8692..8711)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8902..8923
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9086..9190
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9191..9259,9260..10051)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9409..9428)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10222..10810
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10711..10730
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10964..10981
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11098..11119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11134..11139,11140..11157,11158..11164)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11172..11188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11177..11199
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11196..11212
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11196..11212
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11231..11251
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11233..11251
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccttacgac
     2161 gttcctgatt acgcgggata tccctacgac gtgccagatt acgcgggtag tggtggcgac
     2221 ccagcccccg cccgcagcct ggacgacatc gacctgtccg ccctgcggga ccctgctggg
     2281 atctttgagc ttgtggaggt ggtcggcaat ggaacctacg gacaggtgta caagggtcgg
     2341 catgtcaaga cggggcagct ggctgccatc aaggtcatgg atgtcacgga ggacgaggag
     2401 gaagagatca aacaggagat caacatgctg aaaaagtact ctcaccaccg caacatcgcc
     2461 acctactacg gagccttcat caagaagagc cccccgggaa acgatgacca gctctggctg
     2521 gtgatggagt tctgtggtgc tggttcagtg actgacctgg taaagaacac aaaaggcaac
     2581 gccctgaagg aggactgtat cgcctatatc tgcagggaga tcctcagggg tctggcccat
     2641 ctccatgccc acaaggtgat ccatcgagac atcaaggggc agaatgtgct gctgacagag
     2701 aatgctgagg tcaagctagt ggattttggg gtgagtgctc agctggaccg caccgtgggc
     2761 agacggaaca ctttcattgg gactccctac tggatggctc cagaggtcat cgcctgtgat
     2821 gagaaccctg atgccaccta tgattacagg agtgatattt ggtctctagg aatcacagcc
     2881 atcgagatgg cagagggagc cccccctctg tgtgacatgc accccatgcg agccctcttc
     2941 ctcattcctc ggaaccctcc gcccaggctc aagtccaaga agtggtctaa gaagttcatt
     3001 gacttcattg acacatgtct catcaagact tacctgagcc gcccacccac ggagcagcta
     3061 ctgaagtttc ccttcatccg ggaccagccc acggagcggc aggtccgcat ccagcttaag
     3121 gaccacattg accgatcccg gaagaagcgg ggtgagaaag aggagacaga atatgagtac
     3181 agcggcagcg aggaggaaga tgacagccat ggagaggaag gagagccaag ctccatcatg
     3241 aacgtgcctg gagagtcgac tctacgccgg gagtttctcc ggctccagca ggaaaataag
     3301 agcaactcag aggctttaaa acagcagcag cagctgcagc agcagcagcg agaccccgag
     3361 gcacacatca aacacctgct gcaccagcgg cagcggcgca tagaggagca gaaggaggag
     3421 cggcgccgcg tggaggagca acagcggcgg gagcgggagc agcggaagct gcaggagaag
     3481 gagcagcagc ggcggctgga ggacatgcag gctctgcggc gggaggagga gcggcggcag
     3541 gcggagcgcg agcaggaata caagcggaag cagctggagg agcagcggca gtcagaacgt
     3601 ctccagaggc agctgcagca ggagcatgcc tacctcaagt ccctgcagca gcagcaacag
     3661 cagcagcagc ttcagaaaca gcagcagcag cagctcctgc ctggggacag gaagcccctg
     3721 taccattatg gtcggggcat gaatcccgct gacaaaccag cctgggcccg agaggtagaa
     3781 gagagaacaa ggatgaacaa gcagcagaac tctcccttgg ccaagagcaa gccaggcagc
     3841 acggggcctg agccccccat cccccaggcc tccccagggc ccccaggacc cctttcccag
     3901 actcctccta tgcagaggcc ggtggagccc caggagggac cgcacaagag cctggtggca
     3961 caccgggtcc cactgaagcc atatgcagca cctgtacccc gatcccagtc cctgcaggac
     4021 cagcccaccc gaaacctggc tgccttccca gcctcccatg accccgaccc tgccatcccc
     4081 gcacccactg ccacgcccag tgcccgagga gctgtcatcc gccagaattc agaccccacc
     4141 tctgaaggac ctggccccag cccgaatccc ccagcctggg tccgcccaga taacgaggcc
     4201 ccacccaagg tgcctcagag gacctcatct atcgccactg cccttaacac cagtggggcc
     4261 ggagggtccc ggccagccca ggcagtccgt gccagtaacc ccgacctcag gaggagcgac
     4321 cctggctggg aacgctcgga cagcgtcctt ccagcctctc acgggcacct cccccaggct
     4381 ggctcactgg agcggaaccg cgtgggagtc tcctccaaac cggacagctc ccctgtgctc
     4441 tcccctggga ataaagccaa gcccgacgac caccgctcac ggccaggccg gcccgcagac
     4501 tttgtgttgc tgaaagagcg gactctggac gaggcccctc ggcctcccaa gaaggccatg
     4561 gactactcgt cgtccagcga ggaggtggaa agcagtgagg acgacgagga ggaaggcgaa
     4621 ggcgggccag cagaggggag cagagatacc cctgggggcc gcagcgatgg ggatacagac
     4681 agcgtcagca ccatggtggt ccacgacgtc gaggagatca ccgggaccca gcccccatac
     4741 gggggcggca ccatggtggt ccagcgcacc cctgaagagg agcggaacct gctgcatgct
     4801 gacagcaatg ggtacacaaa cctgcctgac gtggtccagc ccagccactc acccaccgag
     4861 aacagcaaag gccaaagccc accctcgaag gatgggagtg gtgactacca gtctcgtggg
     4921 ctggtaaagg cccctggcaa gagctcgttc acgatgtttg tggatctagg gatctaccag
     4981 cctggaggca gtggggacag catccccatc acagccctag tgggtggaga gggcactcgg
     5041 ctcgaccagc tgcagtacga cgtgaggaag ggttctgtgg tcaacgtgaa tcccaccaac
     5101 acccgggccc acagtgagac ccctgagatc cggaagtaca agaagcgatt caactccgag
     5161 atcctctgtg cagccctttg gggggtcaac ctgctggtgg gcacggagaa cgggctgatg
     5221 ttgctggacc gaagtgggca gggcaaggtg tatggactca ttgggcggcg acgcttccag
     5281 cagatggatg tgctggaggg gctcaacctg ctcatcacca tctcagggaa aaggaacaaa
     5341 ctgcgggtgt attacctgtc ctggctccgg aacaagattc tgcacaatga cccagaagtg
     5401 gagaagaagc agggctggac caccgtgggg gacatggagg gctgcgggca ctaccgtgtt
     5461 gtgaaatacg agcggattaa gttcctggtc atcgccctca agagctccgt ggaggtgtat
     5521 gcctgggccc ccaaacccta ccacaaattc atggccttca agtcctttgc cgacctcccc
     5581 caccgccctc tgctggtcga cctgacagta gaggaggggc agcggctcaa ggtcatctat
     5641 ggctccagtg ctggcttcca tgctgtggat gtcgactcgg ggaacagcta tgacatctac
     5701 atccctgtgc acatccagag ccagatcacg ccccatgcca tcatcttcct ccccaacacc
     5761 gacggcatgg agatgctgct gtgctacgag gacgagggtg tctacgtcaa cacgtacggg
     5821 cgcatcatta aggatgtggt gctgcagtgg ggggagatgc ctacttctgt ggcctacatc
     5881 tgctccaacc agataatggg ctggggtgag aaagccattg agatccgctc tgtggagacg
     5941 ggccacctcg acggggtctt catgcacaaa cgagctcaga ggctcaagtt cctgtgtgag
     6001 cggaatgaca aggtgttttt tgcctcagtc cgctctgggg gcagcagcca agtttacttc
     6061 atgactctga accgtaactg catcatgaac tggtgagcaa ttccgcccct ctccctcccc
     6121 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     6181 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     6241 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     6301 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     6361 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     6421 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     6481 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     6541 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     6601 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     6661 ttgaaaaaca cgatgataat atggccacaa ccatgaagac cttcaacatc tctcagcagg
     6721 accttgagct ggtggaagtt gccactgaga aaatcaccat gctctatgag gacaacaagc
     6781 accatgttgg tgctgccatc aggaccaaga caggagaaat catctctgct gtccacattg
     6841 aagcctacat tggcagggtc actgtgtgtg cagaggccat tgccattggg tctgcagtct
     6901 ccaatgggca gaaggacttt gacaccattg tggctgtgag gcacccctac agtgatgagg
     6961 tggacaggtc catcagagtg gtgtccccct gtggcatgtg cagggagctc atctcagact
     7021 atgcccctga ttgctttgtt ctgattgaga tgaatggcaa gctggtcaag acaaccattg
     7081 aggagctgat tccactgaaa tacaccagaa actaaacgcg ttaagtcgac aatcaacctc
     7141 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7201 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7261 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7321 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7381 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7441 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7501 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7561 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7621 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7681 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     7741 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7801 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     7861 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7921 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7981 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     8041 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8101 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8161 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8221 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     8281 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8341 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8401 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     8461 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8521 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8581 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8641 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8701 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8761 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8821 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8881 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     8941 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     9001 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     9061 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9121 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9181 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     9241 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9301 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9361 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9421 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9481 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9541 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9601 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9661 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9721 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9781 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9841 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9901 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9961 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    10021 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10081 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10141 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10201 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    10261 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10321 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    10381 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10441 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10501 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10561 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10621 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10681 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10741 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10801 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10861 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10921 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10981 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    11041 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    11101 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    11161 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    11221 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagc
//
