LOCUS       sequence-47000-e        2320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Seifert HS, Chen EY, So M, Heffron F
  TITLE     Shuttle mutagenesis: a method of transposon mutagenesis for 
            Saccharomyces cerevisiae.
  JOURNAL   Proc Natl Acad Sci U S A. 1986 Feb;83(3):735-9.
  PUBMED    3003748
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1986-02"; volume: "83(3)"; pages: "735-9"
COMMENT     Sequence Label: pHSS6
FEATURES             Location/Qualifiers
     source          1..2320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(294..1088)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(405..424)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1015..1034
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(1514..1531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1685..2272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1765..1784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgcgg aattctcatg tttgacagct tatcatcgat aagcttctag agatcttcca
       61 tacctaccag ttctccgcct gcagcaatgg caacaacgtt gcccggatcc gcggccgccc
      121 ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
      181 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
      241 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
      301 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
      361 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
      421 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
      481 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
      541 tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
      601 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
      661 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
      721 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
      781 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
      841 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
      901 tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
      961 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     1021 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     1081 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat
     1141 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
     1201 gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc
     1261 tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt
     1321 gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct
     1381 ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa
     1441 gctagcttat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     1501 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1561 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1621 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1681 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1741 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1801 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1861 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1921 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1981 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2041 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2101 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2161 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2221 gcggtttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2281 tttgatcttt tcttactgaa cggtgatccc caccggaatt
//
