LOCUS       sequence-470004-        2065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRAV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2065)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRAV23/DV6
FEATURES             Location/Qualifiers
     source          1..2065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(395..411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(395..411)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      450..481
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        482..584
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             585..1244
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(651..670)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1268..1295
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1307..1894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1387..1406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1916..1945)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     2018..2035
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2019..2035
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcagat ggacaagatc ttaggagcat catttttagt tctgtggctt caactatgct
       61 gggtgagtgg ccaacagaag gagaaaagtg accagcagca ggtgaaacaa agtcctcaat
      121 ctttgatagt ccagaaagga gggatttcaa ttataaactg tgcttatgag aacactgcgt
      181 ttgactactt tccatggtac caacaattcc ctgggaaagg ccctgcatta ttgatagcca
      241 tacgtccaga tgtgagtgaa aagaaagaag gaagattcac aatctccttc aataaaagtg
      301 ccaagcagtt ctcattgcat atcatggatt cccagcctgg agactcagcc acctacttct
      361 gttgagacgc agggaagatg gccgcgaaga aaacgtcata gctgtttcct gactgaccat
      421 ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac
      481 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
      541 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
      601 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      661 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
      721 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
      781 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
      841 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
      901 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
      961 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg ttttttgtct
     1021 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1081 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     1141 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     1201 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatgaggg cccaaatgta
     1261 atcacctggc tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc cataggctcc
     1321 gcccccctga cgagcatcac aaaaatcgat gctcaagtca gaggtggcga aacccgacag
     1381 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1441 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1501 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1561 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1621 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1681 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1741 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttacctcg gaaaaagagt
     1801 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1861 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgattt tctaccgaag
     1921 aaaggcccac ccgtgaaggt gagccagtga gttgattgca gtccagttac gctggagtct
     1981 gaggctcgtc ctgaatgata tcaagcttga attcgtttgt aaaacgacgg ccagtgctaa
     2041 ccgtatcgca ttggccatac ctaca
//
