LOCUS       sequence-47003-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Seifert HS, Chen EY, So M, Heffron F
  TITLE     Shuttle mutagenesis: a method of transposon mutagenesis for 
            Saccharomyces cerevisiae.
  JOURNAL   Proc Natl Acad Sci U S A. 1986 Feb;83(3):735-9.
  PUBMED    3003748
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1986-02"; volume: "83(3)"; pages: "735-9"
COMMENT     Sequence Label: pHSS6
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(701..720)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
      241 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataagc tagcttcacg
      301 ctgccgcaag cactcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag
      361 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
      421 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
      481 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
      541 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
      601 caggggatca agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
      661 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
      721 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
      781 ggt
//
