LOCUS       sequence-470035-        2022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRAV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2022)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRAV4
FEATURES             Location/Qualifiers
     source          1..2022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(356..372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(356..372)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      411..442
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        443..545
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             546..1205
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(612..631)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1229..1256
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1268..1851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1348..1367)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1873..1902)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1975..1992
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1976..1992
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcagat gaggcaagtg gcgagagtga tcgtgttcct gaccctgagt actttgagcc
       61 ttgctaagac cacccagccc atctccatgg actcatatga aggacaagaa gtgaacataa
      121 cctgtagcca caacaacatt gctacaaatg attatatcac gtggtaccaa cagtttccca
      181 gccaaggacc acgatttatt attcaaggat acaagacaaa agttacaaac gaagtggcct
      241 ccctgtttat ccctgccgac agaaagtcca gcactctgag cctgccccgg gtttccctga
      301 gcgacactgc tgtgtactac tgttgagacg cagggaagat ggccgcgaag aaaacgtcat
      361 agctgtttcc tgactgacca tttaaatcat acctgacctc catagcagaa agtcaaaagc
      421 ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt caccataatg
      481 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
      541 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
      601 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
      661 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
      721 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag
      781 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
      841 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
      901 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
      961 ttgagaatat gttttttgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     1021 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     1081 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     1141 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     1201 cgtaatgagg gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc
     1261 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga tgctcaagtc
     1321 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1381 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1441 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1501 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1561 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1621 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1681 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1741 cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1801 cggtggtttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1861 ttgattttct accgaagaaa ggcccacccg tgaaggtgag ccagtgagtt gattgcagtc
     1921 cagttacgct ggagtctgag gctcgtcctg aatgatatca agcttgaatt cgtttgtaaa
     1981 acgacggcca gtgctaaccg tatcgcattg gccataccta ca
//
