LOCUS       sequence-470049-        3381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRBV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3381)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRBV6-1
FEATURES             Location/Qualifiers
     source          1..3381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(373..389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(373..389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      428..459
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             1901..2560
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1967..1986)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2584..2611
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2623..3210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2703..2722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3232..3261)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     3334..3351
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3335..3351
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcacca tgagcatcgg gctcctgtgc tgtgtggcct tttctctcct gtgggcaagt
       61 ccagtgaatg ctggtgtcac tcagacccca aaattccagg tcctgaagac aggacagagc
      121 atgacactgc agtgtgccca ggatatgaac cataactcca tgtactggta tcgacaagac
      181 ccaggcatgg gactgaggct gatttattac tcagcttctg agggtaccac tgacaaagga
      241 gaagtcccca atggctacaa tgtctccaga ttaaacaaac gggagttctc gctcaggctg
      301 gagtcggctg ctccctccca gacatctgtg tacttctgtg tgagacgcag ggaagatggc
      361 cgcgaagaaa acgtcatagc tgtttcctga ctgaccattt aaatcatacc tgacctccat
      421 agcagaaagt caaaagcctc cgaccggagg cttttgactt gatcggcacg taagaggttc
      481 caactttcac cataatgaaa taagatcact accgggcgct gatgaatccc ctaatgattt
      541 tggtaaaaat cattaagtta aggtggatac acatcttgtc atatgatcaa atggtttcgc
      601 gaaaaatcaa taatcagaca acaagatgtg cgaactcgat attttacacg actctcttta
      661 ccaattctgc cccgaattac acttaaaacg actcaacagc ttaacgttgg cttgccacgc
      721 attacttgac tgtaaaactc tcactcttac cgaacttggc cgtaacctgc caaccaaagc
      781 gagaacaaaa cataacatca aacgaatcga ccgattgtta ggtaatcgtc acctccacaa
      841 agagcgactc gctgtatacc gttggcatgc tagctttatc tgttcgggca atacgatgcc
      901 cattgtactt gttgactggt ctgatattcg tgagcaaaaa cgacttatgg tattgcgagc
      961 ttcagtcgca ctacacggtc gttctgttac tctttatgag aaagcgttcc cgctttcaga
     1021 gcaatgttca aagaaagctc atgaccaatt tctagccgac cttgcgagca ttctaccgag
     1081 taacaccaca ccgctcattg tcagtgatgc tggctttaaa gtgccatggt ataaatccgt
     1141 tgagaagctg ggttggtact ggttaagtcg agtaagagga aaagtacaat atgcagacct
     1201 aggagcggaa aactggaaac ctatcagcaa cttacatgat atgtcatcta gtcactcaaa
     1261 gactttaggc tataagaggc tgactaaaag caatccaatc tcatgccaaa ttctattgta
     1321 taaatctcgc tctaaaggcc gaaaaaatca gcgctcgaca cggactcatt gtcaccaccc
     1381 gtcacctaaa atctactcag cgtcggcaaa ggagccatgg gttctagcaa ctaacttacc
     1441 tgttgaaatt cgaacaccca aacaacttgt taatatctat tcgaagcgaa tgcagattga
     1501 agaaaccttc cgagacttga aaagtcctgc ctacggacta ggcctacgcc atagccgaac
     1561 gagcagctca gagcgttttg atatcatgct gctaatcgcc ctgatgcttc aactaacatg
     1621 ttggcttgcg ggcgttcatg ctcagaaaca aggttgggac aagcacttcc aggctaacac
     1681 agtcagaaat cgaaacgtac tctcaacagt tcgcttaggc atggaagttt tgcggcattc
     1741 tggctacaca ataacaaggg aagacttact cgtggctgca accctactag ctcaaaattt
     1801 attcacacat ggttacgctt tggggaaatt atgaggggat ctctcagtac cgggcgtatt
     1861 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     1921 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1981 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     2041 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     2101 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     2161 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     2221 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     2281 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt ttgtctcagc
     2341 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     2401 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     2461 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     2521 attacaacag tactgcgatg agtggcaggg cggggcgtaa tgagggccca aatgtaatca
     2581 cctggctcac cttcgggtgg gcctttctgc gttgctggcg tttttccata ggctccgccc
     2641 ccctgacgag catcacaaaa atcgatgctc aagtcagagg tggcgaaacc cgacaggact
     2701 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2761 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2821 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2881 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2941 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3001 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3061 aagaacagta tttggtatct gcgctctgct gaagccagtt acctcggaaa aagagttggt
     3121 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3181 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgattttcta ccgaagaaag
     3241 gcccacccgt gaaggtgagc cagtgagttg attgcagtcc agttacgctg gagtctgagg
     3301 ctcgtcctga atgatatcaa gcttgaattc gtttgtaaaa cgacggccag tgctaaccgt
     3361 atcgcattgg ccatacctac a
//
