LOCUS       sequence-470050-        2042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRBV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2042)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRBV6-4
FEATURES             Location/Qualifiers
     source          1..2042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(373..389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(373..389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      428..459
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        460..562
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             563..1222
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(629..648)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1245..1272
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1284..1871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1364..1383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1893..1922)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1995..2012
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1996..2012
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcacca tgagaatcag gctcctgtgc tgtgtggcct tttctctcct gtgggcaggt
       61 ccagtgattg ctgggatcac ccaggcacca acatctcaga tcctggcagc aggacggcgc
      121 atgacactga gatgtaccca ggatatgaga cataatgcca tgtactggta tagacaagat
      181 ctaggactgg ggctaaggct catccattat tcaaatactg caggtaccac tggcaaagga
      241 gaagtccctg atggttatag tgtctccaga gcaaacacag atgatttccc cctcacgttg
      301 gcgtctgctg taccctctca gacatctgtg tacttctgtg tgagacgcag ggaagatggc
      361 cgcgaagaaa acgtcatagc tgtttcctga ctgaccattt aaatcatacc tgacctccat
      421 agcagaaagt caaaagcctc cgaccggagg cttttgactt gatcggcacg taagaggttc
      481 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      541 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      601 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      661 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      721 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      781 tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      841 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      901 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      961 tttccctaaa gggtttattg agaatatgtt ttttgtctca gccaatccct gggtgagttt
     1021 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1081 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1141 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1201 tgagtggcag ggcggggcgt aatgagggcc caaagtaatc acctggctca ccttcgggtg
     1261 ggcctttctg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1321 aatcgatgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1381 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1441 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1501 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1561 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1621 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1681 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1741 tgcgctctgc tgaagccagt tacctcggaa aaagagttgg tagctcttga tccggcaaac
     1801 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1861 aaggatctca agaagatcct ttgattttct accgaagaaa ggcccacccg tgaaggtgag
     1921 ccagtgagtt gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca
     1981 agcttgaatt cgtttgtaaa acgacggcca gtgctaaccg tatcgcattg gccataccta
     2041 ca
//
