LOCUS       sequence-470052-        9952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of Paracingulin (dog)  in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Paschoud S, Citi S
  TITLE     Inducible overexpression of cingulin in stably transfected MDCK 
            cells does not affect tight junction organization and gene 
            expression.
  JOURNAL   Mol Membr Biol. 2008 Jan;25(1):1-13. doi: 10.1080/09687680701474009.
  PUBMED    18097951
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Membr Biol"; date: "2008-01"; volume: "25"; issue: "1"; pages: 
            "1-13"
COMMENT     Sequence Label: YFP-cCGNL1-Myc
FEATURES             Location/Qualifiers
     source          1..9952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1140..1159
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1158..1213
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1259..1278
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     intron          1410..1982
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             complement(2022..2051)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5628..5639
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(join(5952..6662,6663..6665,6666..6668))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5994..6015)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6343..6362
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6602..6623
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6665..6674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6774..6798)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6795..6833)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(6866..6884,6885..6907,6908..6926,6927..6949,6950..6968,
                     6969..6991,6992..7010,7011..7033,7034..7052,7053..7075,
                     7076..7094,7095..7117,7118..7136)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        7150..7479
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7330..7465
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7488..8513
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7488..8513
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8630..8711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8667..8686)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8731..8749)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8817..8921
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8922..8990,8991..9782)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9140..9159)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagtcgag ggatctccat
      841 aagagaagag ggacagctat gactgggagt agtcaggaga ggaggaaaaa tctggctagt
      901 aaaacatgta aggaaaattt tagggatgtt aaagaaaaaa ataacacaaa acaaaatata
      961 aaaaaaatct aacctcaagt caaggctttt ctatggaata aggaatggac agcagggggc
     1021 tgtttcatat actgatgacc tctttatagc caacctttgt tcatggcagc cagcatatgg
     1081 gcatatgttg ccaaactcta aaccaaatac tcattctgat gttttaaatg atttgccctc
     1141 ccatatgtcc ttccgagtga gagacacaaa aaattccaac acactattgc aatgaaaata
     1201 aatttccttt attagccaga agtcagatgc tcaaggggct tcatgatgtc cccataattt
     1261 ttggcagagg gaaaaagatc tcagtggtat ttgtgagcca gggcattggc cacaccagcc
     1321 accaccttct gataggcagc ctgcacctga ggagtgaatt ctttgccaaa atgatgagac
     1381 agcacaacaa ccagcacgtt gcccaggagc tgtaggaaag agaagaaggc atgaacatgg
     1441 ttagcagagg ggcccggttt ggactcagag tattttatcc tcatctcaaa cagtgtatat
     1501 cattgtaacc ataaagagaa aggcaggatg atgaccaggg tgtagttgtt tctaccaata
     1561 agaatatttc cacgccagcc agaatttata tgcagaaata ttctacctta tcatttaatt
     1621 ataacaattg ttctctaaaa ctgtgctgaa gtacaatata atataccctg attgccttga
     1681 aaaaaaagtg attagagaaa gtacttacaa tctgacaaat aaacaaaagt gaatttaaaa
     1741 attcgttaca aatgcaagct aaagtttaac gaaaaagtta cagaaaatga aaagaaaata
     1801 agaggagaca atggttgtca acagagtaga aagtgaaaga aacaaaatta tcatgagggt
     1861 ccatggtgat acaagggaca tcttcccatt ctaaacaaca ccctgaaaac tttgccccct
     1921 ccatataaca tgaattttac aatagcgaaa aagaaagaac aatcaagggt ccccaaactc
     1981 accctgaagt tctcagctct agagatatcg tcgacctaat tcagatcctc ttctgagatg
     2041 agtttttgtt catcgacgat ctggctggtg gcagcgctgt ccttggggaa ggtgtagctc
     2101 agcggcgcct cgtggaggct ccccccgtcc gtactgaggt catcatcatc cgtgtcgtcc
     2161 agcactttgc tcggcagctt cttgagtctt aggtccttct tcatggaatt gagttgtccc
     2221 tgcagctgct cattcacatc catctgctcc tccagctccc tctgcagctt ctttttagaa
     2281 ctttctagtc tgtcgatctc ctcctcggcc tcctctactt gtcgtttcat tgctttcaag
     2341 cgcaagctaa gctggtcctt ctgatcagtc agagacaggt gctcatcgtc cacttgcatc
     2401 actagctcct tcaccttccg ctccagccgt cggttgctga gctggaggct ggcccggtcc
     2461 ctctcctcgt tttccagacg gtcctccagc tccgcgatcc tggcctccat ctgcaccacc
     2521 agcccctctt tgctggacct gtaggagcct tccagatgga taatccggct ctttaaatct
     2581 ttgttctgcc tctccaagga aatcttgtca cactccaagt cctgcttgag agctctttcc
     2641 tgcaggagct cgctcctcat ctgctccatc tgttccctgc tccgagtgat cctctccgag
     2701 agcaaatccg agttatttct ttcttcttcc agttccatct ccagctgaga caccttgtcc
     2761 tccatctgct tgacgagtct gcttcggtca tctttgaggt gacttttggc ctccagctcg
     2821 tattccaggt ccttgagtgt ctgctccaga agctgccttt ttgtgagcgc ctcatcctga
     2881 gctctctggt agtcccgtaa ctcctcctcc atcagacgca tctcatcctg cagtttcatg
     2941 gcagtcagac gtgacttttc agcctctagc gttttctcct tcaactgcct ttgcatttct
     3001 gcaagttccc tgcgattttt ctccttgtac tcatctagct ggttctggag ctccagtgtt
     3061 gatacacggg acacctcaac aatgtcagcc atctctttct gcaatttttc aatggtcttg
     3121 tccagatgcc atcgctcgtt ctccatctcg ttcttcagcc tccttaactg ctctttctgc
     3181 tcagtctcat ctttaagctt ctcagacaac tgcttctgct cctgggtggc ccgactcaga
     3241 tttctctgca cgtcttccag ctcaccctcc agggtccttc tgactgatgt ggcttctctt
     3301 tcttcctttc ttgcattcac aagggcctcc tctaattgcc ggttttctct ctcgcatttt
     3361 gtgagccttt cctttgcctt gacctcatcg tccttcatgt cagcaatctg cctctgcagc
     3421 cgagcgacgc tctgctccag ctcttcgatg ctttcccgca gcttctcatt ctcctcctgc
     3481 agagccttca cgtgcatttc agcccccagc tggctctgct ctgaagtgtt gctccgactg
     3541 gccaggacct cgacgttctt ggttgcttct tctacactct ccctcagggc ctgcagctcg
     3601 gcgtcatact gctccttcag cttatccatc tccaggtcgt ggctggacac ctcttccttc
     3661 agggctccct tcagggcggt cagttcacgc tcccgcttcc tcaagaggtc ctcctgctcc
     3721 tctttggcga tgagcagatc ctgaagatcc tgttttgcct gtaagagttc ctcaatcagg
     3781 gctccctttt ccctgtcctc agatcgttta gcactgtcca attcatcgtg catttctgag
     3841 agctggtcct ggagatccct gatctcagtc tggtgctgtt cccgttccat cttcacctgg
     3901 aacaattcct ccaggttctt gcggagctcc ccttcactct cttccaggcg ctgctgtagc
     3961 atggagttct cttccacttt actatcatgc tggccctgga gctcctccaa atttgctttc
     4021 attctctctc tctcttcttt gatgttctgc tgattcttga cttctagttg aagctgtctc
     4081 tgaagttcag ccacttctgt ggtcagcttg tttttctgtt ccagcagatc tctgacttca
     4141 gatgaagaat tagaagcttg attatttcct tgtgtgctcc ctgctgctcg agactttaaa
     4201 gtctggattt tctcaaagac caggttgact ttccgtttag tcgcatcatc attatcggtg
     4261 cttccttctt tgaggtaatt gtaaagtatc tgcttggccg tctcctcatt ggtttgttga
     4321 gtgagctctt gttggccttt caacagatct ggtgtggcct gagtgttgga ggcggaagta
     4381 aatgtcttca cagaaatctt cttggcacca gagtcgggcg aagtcgctag ggcccgattc
     4441 tgtaacatca gcgtcgccgt ggctgtcttg atttcgtcct cctttttatt ctgggctggg
     4501 ggggaggaca cacgcgtgtt tgtactctcc cgaccccctc tgtttgctgg agctttccca
     4561 tctttgctct gctgggcagg ccggaggtgg gccccctgca gcttggcgaa ggcgcggccc
     4621 acgctctgtc tcccgcggtg ctcctgggca ggtgaccgct gcgggcacac ctgcgacggg
     4681 cggaggaggt gttcgctgga cttgcccagg ttccggctaa attcgctgag gtactccgag
     4741 ttcccctgca gaccgaaagg aaaggcacta tccacgcttc tggacctctt cctgtcttct
     4801 ggattgattc tgttccgctt cccaggcctt cctctcctct gaagcccggg tttttggtca
     4861 aacttttcaa tcagttgatc cacaccagga attgatcccg tgtcaatatc ccgcccagtt
     4921 cctggcagga aggggatata ccgtctgttt tcgtggcgat tcacattgtc agcgtggatg
     4981 gcacattcct gatcatcaag cagaaatctg tacaaagagt tggccgacgt gggggttgcc
     5041 gacgaggaag aggacctccg ggaccgagct ccgtccagga caggtcccgc tgaatcttgt
     5101 cgccggaagg gaagcacgtc cggcctggct ttcttggctt caggatgggt gtgcgggctg
     5161 tgggcagtgg gggacctccc actaggggaa ggggcctccc ctaccagcgc ctccccggtg
     5221 cagctctcaa catttacgca cactgaggtc tgcttcttgg ggtcatcgat gaccacagag
     5281 ctgcacaaac ggatggccgt cacgccggat ttgcccgcat cttccaaagg agggctggta
     5341 ggctgggaac taccaggctt ggggaagcag gtccatgcct tcttattgtt ggtaccttct
     5401 tctgtcaatg tgtttagcca attatgctca ggaggctggt ggttttgcaa gttcgattca
     5461 ttcttttcag ggtcataggg ctgcagaagc tctggatgcc tctgaaagtt caacacgtgg
     5521 gatggcttca ttggcccctc tttgcgttct agaaccccgt tcttgccttc gaagagagag
     5581 gactgtttca ggtttcttac tgggctgggc tgggcatacg gattttctgg gagctgcaat
     5641 tcctctgcgc tgctctcctc cagcacggac ccgttgctgg aatgcagagg caggttactt
     5701 ctcattggag atggaaatgg tggcccattt tcaggaaaag atgtgcctgc cagacaccgc
     5761 tctgtgttat tcaggactat gtaggggtga ccatcgattc cctgcacccg aatactgaca
     5821 ccataggagc ctgccttaga gtgttgggaa ttccttggct ttttgctatc atcgcttgcg
     5881 agtctcagat ggacgccata ttcttgctgt acgtgttggt attcgccgaa atacagctcc
     5941 atgcggccgc tcttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
     6001 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactggtag
     6061 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
     6121 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
     6181 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
     6241 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
     6301 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
     6361 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
     6421 tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact gcaggccgta gccgaaggtg
     6481 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
     6541 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
     6601 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
     6661 ctcaccatgg tggcggatcc ccgggtaccg agctcgaatt cggggccgcg gaggctggat
     6721 cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga
     6781 cggttcacta aacgagctct gcttatatag gcctcccacc gtacacgcct acctcgaccc
     6841 gggtaccgag ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
     6901 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
     6961 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
     7021 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
     7081 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     7141 taaactcgag tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7201 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7261 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7321 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7381 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7441 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttccatg aaaaagcctg
     7501 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     7561 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     7621 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     7681 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     7741 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     7801 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     7861 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     7921 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     7981 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     8041 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     8101 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     8161 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     8221 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     8281 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     8341 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     8401 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     8461 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagttcta
     8521 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     8581 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     8641 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8701 tttttcactg cctcgagtga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     8761 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     8821 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8881 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8941 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9001 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9061 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9121 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     9181 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9241 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9301 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9361 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9421 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9481 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9541 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9601 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9661 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9721 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9781 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9841 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9901 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
