LOCUS       sequence-470060-        8034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Library-scale IPTG-inducible gRNA expression for Type II dCas9 in 
            Synechococcus sp. PCC 7002, genomically integrated next to glpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Hren A, Lollini N, Carper DL, Abraham PE, Cameron JC, Fox JM, Eckert
            CA
  TITLE     High-density CRISPRi screens reveal diverse routes to improved 
            acclimation in cyanobacteria.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Mar 25;122(12):e2412625122. doi: 
            10.1073/pnas.2412625122. Epub 2025 Mar 21.
  PUBMED    40117303
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-03-25"; volume: "122(12):e2412625122. 
            doi"; pages: " 10.1073/pnas.2412625122. Epub 2025 Mar 21"
COMMENT     Sequence Label: pAPH07
FEATURES             Location/Qualifiers
     source          1..8034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     432..451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(670..774)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     842..860
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(898..920)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1020..1039
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1233..1255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1247..1264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1248..1264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1703..1714)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(2614..3696)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDFSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3658..3677
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3726..3760)
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     protein_bind    3787..3806
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3891..3907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_RNA        3953..4028
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4049..4078
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4094..4111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4167..4184)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(4480..4499)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4491..5306
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             4491..5306
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4564..4583)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(6682..6698)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6682..6698)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    6706..6722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6730..6736,6737..6754,6755..6760))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6775..6796
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6913..6930)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7084..7672)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7164..7183)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7843..8034,1..600,601..669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
       61 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      121 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      181 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      241 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      301 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      361 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      421 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      481 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      541 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      601 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      661 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      721 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      781 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
      841 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
      901 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
      961 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     1021 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     1081 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     1141 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1201 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1261 cagtgaattc gagctcggta cccgggggac ggctcccctc tgtcaaaccc tcagtgcggc
     1321 aggcaaagcg gcagaaattt acgataaaac gggcttggtg ttggatgctt atttttcggc
     1381 gacgaaactg aattggttat taagctgggc taaggaaaat agtgccatta accccaataa
     1441 tgtcctcgct ggaaccattg acacctgggc cttgtggaac ctcacgggag gcaaagtcca
     1501 cgccacagat cacagtaatg ccagccggac aatgttgctc aatctcaacc aaaaagattg
     1561 ggatccggat ttgttggatt tattcgatat tccccgccag atgatgccaa cggtgcagtc
     1621 gagcctaggg gaatttggca aaaccgatcc aagtttattg ggggcggcaa ttccgatcac
     1681 cgccatcttt ggggatcaac aggcggccct ctatgcccat ggttgcgatc gccccggctt
     1741 attaaaatgc acctatggca caggggcgtt tttggtggcg cacacgggcg acgagattaa
     1801 acgctccaaa cataagctcc taacgaccat cgcctgcacc caagcccatc acgatggcac
     1861 aaatctcgat attggctatg ccctcgaagg gagtatgttc accgctgggg cttgtatcca
     1921 atggttgcgc gatggcctac aaattatcga aacggcggcg gaaacgaatg atcttgccca
     1981 aggggtgaat gataatggcg gcgcttattt tgttccggct ttaagtggtc tgggagcgcc
     2041 ccactgggat atgagtgcca ggggcgcatt tctgggttta acgagaggcg tcaaaaaaga
     2101 acacatggta cgggctgtcc tagaggcgat cgcctaccaa gccaaagaag tagtcgaagc
     2161 gattaaccaa gattccggta cgccgatcca agaattaaaa gtcgatggcg gcgcgtgcaa
     2221 taacgacttt ctgatgcaat tccaagctga cgtgttgggt attcccgtgg aacgtcccgc
     2281 tgtcctcgat gccacggccc agggggcagc ctttgccgct ggattagctg tcggtttttg
     2341 ggatgattac caaaccctgg tgcaaaaccg caaaattgat tacgtcttta agcccagtgc
     2401 caacgcctcc caagcccaag cccatttcaa agtctggcaa aaagccgttg aacgagcgaa
     2461 aaactgggcc taaccccctc ttgcctacag catctccccc aggggagaat tcttcctgtt
     2521 tcaactcccc aagcttggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa
     2581 tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2641 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2701 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
     2761 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
     2821 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
     2881 tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
     2941 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
     3001 catttgcatg gtttgttgaa aaccggacat ggcactaaag tcgccttccc gttccgctat
     3061 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
     3121 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
     3181 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
     3241 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
     3301 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
     3361 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
     3421 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
     3481 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
     3541 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
     3601 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
     3661 atactctgcg acatcgtata acgttactgg tttcacattc accaccatga attgaccctc
     3721 ctccggctag cattgtacct aggactgagc tagctgtaaa gactcccctc tggacatctc
     3781 caaacgaatt gtgagcgctc acaattcgga attcttaaca aaaaagcagg aataaaatta
     3841 acaagatgta acagacataa gtcccatcac cgttgtataa atgtgtggaa ttgtgagcgg
     3901 ataacaattt cacacatgaa gagccgtgat ggcacttaga actggctctt cagttttaga
     3961 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     4021 gtcggtgctt tttttgaagc ttgggcccga acaaaaactc atctcagaag aggatctgaa
     4081 tagcgccgtc gaccatcatc atcatcatca ttgagtttaa acggtctcca gcttggctgt
     4141 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     4201 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     4261 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     4321 gggaactgcc aggcatcaaa taaaacgaaa gactgggcct ttcgttttat ctgttgtttg
     4381 tcggtgaact acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4441 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     4501 ttcaacggga aacgtcttgc tctaggccgc gattaaattc caacatggat gctgatttat
     4561 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
     4621 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     4681 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     4741 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggga
     4801 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     4861 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     4921 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     4981 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     5041 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     5101 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     5161 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     5221 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     5281 atttgatgct cgatgagttt ttctaagaat taattcatga gcggatacat atttgaatgt
     5341 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaatc taacgtaaac
     5401 ccattgaatt taaaaaagac tttatgactg ctttactgct ccatgaccaa cattattccc
     5461 ttgatcatga agcctttctc tcaaccctca gcaacacaga aaatttactc attattcaag
     5521 atctagatgg cgtttgcatg gggttagtca aagacccctt aacccgcaaa attgatcctg
     5581 actatatccg cgccacacgc aagtttagag accacttctt tgtcctcacc aacggtgaac
     5641 atgaaggcag aaggggagta aatcgcatcg ttgaacgggc atttcgcaat gttgaagcca
     5701 aagaggaaac aagctattta cctggtttag cagcaggggg tgtgcaatgg cagacagata
     5761 atggccaaat ttcccatccc ggtgttagcc aagcagaact cgatttcctt gccacagtgc
     5821 cagatttaat tggtcaaagt ttaggacaat tttttactaa atatgttgat atttttcccg
     5881 ctgagcttca acctgagctg atccatgctt ctgttttaga taatctcgtt tcaccgacgg
     5941 caaatttaaa cgtcctggcc gaatatttag gcgatcgcct tgagatttac caagacctcc
     6001 agcgcaccat ggaaaccctg atgaatgatt tgctagaaaa agctggccaa cagggtttag
     6061 acaatagttt tttcgtgcac tatgcgccca atttaggcag agataatgtg gggaaagaaa
     6121 ttgtccgctt tgccacagcc aaggattctg gcaccacaga ttttcagttt atggtgtgtg
     6181 gtgccgtcaa agaagcgggg gttttagtgc tgctgaatta ttactatgcc caacgcacgg
     6241 gccactatcc cctaggagaa acctttaacg cccgccaagc gccccaaaac cacgaggaac
     6301 tattgcaact ggtgcaagac aatttcgatc cgcaattgat gcctttaatc gttggcgtcg
     6361 gcgatacggt cacaagtcac accgaaggcg atcaagttcg acgggggggg agcgatcgcc
     6421 tattcctgca gctcgtccaa gacattggta aatgggcaaa gagcggcaat ctcgtcgtgt
     6481 atatcgacag ctcccaggga gaattaaaaa atcggattcc cttaaaactg gggacaatca
     6541 atggtcaaac ccaagtgatt gagggcatta ctgatccggc tgatccgtta caaattaacg
     6601 ttgcttttcc gggcgggttt gaacaataca ccgcccttct ctagagtcga cctgcaggca
     6661 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6721 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6781 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6841 gtgccagctg cattaatgaa tcgtccaacg cgcggggaga ggcggtttgc gtattgggcg
     6901 gtcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6961 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7021 gaacatgtga gcaaaaggcc agcaaaaggc cagtaaccgt aaaaaggccg cgttgctggc
     7081 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7141 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7201 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7261 aatcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7321 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7381 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7441 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7501 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7561 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7621 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7681 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7741 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7801 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7861 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7921 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7981 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccgg
//
