LOCUS       sequence-470086-        8420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for sgRNA-S1b and eCas12f1i.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Park SJ, Ju S, Jung WJ, Jeong TY, Yoon DE, Lee JH, Yang J, Lee H, 
            Choi J, Kim HS, Kim K
  TITLE     Robust genome editing activity and the applications of enhanced 
            miniature CRISPR-Cas12f1.
  JOURNAL   Nat Commun. 2025 Jan 15;16(1):677. doi: 10.1038/s41467-025-56048-w.
  PUBMED    39809780
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-01-15"; volume: "16(1):677. doi"; pages: " 
            10.1038/s41467-025-56048-w"
COMMENT     Sequence Label: pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i
FEATURES             Location/Qualifiers
     source          1..8420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        738..842
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(843..911,912..1703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1061..1080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1874..2462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2363..2382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2524..2772
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2524..2544
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2695..2714
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     CDS             complement(3120..3443)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     3338..3360
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3352..3369
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3353..3369
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3370..3426
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3439..3455)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3439..3455)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3452..3474)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3463..3479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3487..3493,3494..3511,3512..3517))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     enhancer        3572..3857
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          4106..4333
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             4387..4407
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6919..7104
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             7117..7164
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             7174..7230
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7231..7233,7234..7236,7237..7950)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7231..7233,7234..7236,7237..7947)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7276..7297)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7537..7556)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7884..7905
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7984..8001)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7990..8197
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8206..8346
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8206..8335
                     /label=AAV2 ITR
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
      361 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
      481 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
      541 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      601 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
      661 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      721 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      781 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      841 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      901 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      961 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1021 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1081 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1141 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1201 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1261 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1321 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1381 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1441 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1501 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggaa
     1561 gccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1621 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1681 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1741 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1801 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1861 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1921 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1981 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2041 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2101 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2161 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2221 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2281 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2341 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2401 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2461 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2521 tgtgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     2581 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     2641 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     2701 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     2761 ggacgaaaca ccgaccgctg gacttcgagt ccaggtgggc tgcttgcatc agcctaatgt
     2821 cgagaagtgc tttcttcgga aagtaaccct cgaaacaaag aaaggaatgc aacggagacc
     2881 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     2941 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     3001 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3061 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     3121 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     3181 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     3241 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3301 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3361 acggccagtg aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
     3421 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3481 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatagatccg
     3541 gtctcctttt attttttgtt ttagaggtac ccgttacata acttacggta aatggcccgc
     3601 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt
     3661 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3721 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3781 attgtgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3841 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     3901 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     3961 gggcgggggg ggggggcgcg cgccaggcgg ggcgaggcgg agaggtgcgg cggcagccaa
     4021 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
     4081 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg
     4141 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     4201 gagcgggcgg gacggccctt ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt
     4261 taagggatgg ttggttggtg gggtattaat gtttaattac ctggagcacc tgcctgaaat
     4321 cacttttttt caggttggac cggtgccacc atgaaacgga cagccgacgg aagcgagttc
     4381 gagtcaccaa agaagaagcg gaaagtcggc cctatggaga tatacaagac agtgtctgca
     4441 tggaagagac agccagtgcg ggtactgagc ctcttcagaa acatcgacaa ggtactaaag
     4501 agtttgggct tcttggaaag cggttctggt tctgggggag gaacgctgaa gtacgtggaa
     4561 gatgtcacaa atgtcgtgag gagagacgtg gagaaatggg gcccctttga cctggtgtac
     4621 ggctcgacgc agcccctagg cagctcttgt gatcgctgtc ccggctggta catgttccag
     4681 ttccaccgga tcctgcagta tgcgctgcct cgccaggaga gtcagcggcc cttcttctgg
     4741 atattcatgg acaatctgct gctgactgag gatgaccaag agacaactac ccgcttcctt
     4801 cagacagagg ctgtgaccct ccaggatgtc cgtggcagag actaccagaa tgctatgcgg
     4861 gtgtggagca acattccagg gctgaagagc aagcatgcgc ccctgacccc aaaggaagaa
     4921 gagtatctgc aagcccaagt cagaagcagg agcaagctgg acgccccgaa agttgacctc
     4981 ctggtgaaga actgccttct cccgctgaga gagtacttca agtatttttc tcaaaactca
     5041 cttcctcttg gagggccgag ctctggcgca cccccaccaa gtggagggtc ccctgccggg
     5101 tccccaacat ctactgaaga aggcaccagc gaatccgcaa cgcccgagtc aggccctggt
     5161 acctccacag aaccatctga aggtagtgcg cctggttccc cagctggaag ccctacttcc
     5221 accgaagaag gcacgtcaac cgaaccaagt gaaggatctg cccctgggac cagcactgaa
     5281 ccatctgagg ccaagaacac aatcaccaag acactgaagc tgagaatcgt gcggccttac
     5341 aacagcgccg aggtggaaaa aatcgtggcc gatgagaaga acaacagaga gaaaatcgct
     5401 ctggaaaaaa ataaggacaa ggttaaagaa gcctgctcca agcacctgaa agtggctgcc
     5461 tactgcacca cccaggtcga gagaaacgcc tgtctgttct gcaaggccag aaagctggac
     5521 gacaagttct accaaaagct gagaggccag ttccccgacg ccgtgttctg gcaggagatt
     5581 agcgagattt tcagacagct gcagaagcag gccgccgaga tctacaacca gagcctgatc
     5641 gagctgtact acgagatctt tatcaaaggc aaaggaattg ccaacgcttc tagcgtggaa
     5701 cactacctga gcagagtgtg ctaccggaga gccgccgagc tgttcaagaa tgccgctatc
     5761 gccagcggac tgcggagcaa gatcaagtct aacttccggc tgaaggaact caagaatatg
     5821 aaatcccggc tgccaacaac aaagagcgac aactttccaa tccccctggt gaagcagaaa
     5881 ggaggccagt ataccggctt tgagatcagc aaccacaact ctgatttcat catcaagatc
     5941 ccattcggca gatggcaagt gaagaaggaa atcgacaagt acagaccttg ggagaagttc
     6001 gacttcgagc aggtgcagaa gagtcctaag cctatcagcc tgctgctgtc tacccagaga
     6061 cggaaacgga acaaaggctg gtccaaggac gagggcaccg aagccgaaat caagaaggtg
     6121 atgaacggcg actaccagac cagctacatc gaagtgaaga ggggcagcaa gatcggcgag
     6181 aagtctgcct ggatgctgaa cctgagcatc gatgttccta agatcgacaa aggagtggac
     6241 cccagcatca tcggcggaat tgccgtgggc gtgagaagcc ctctggtctg tgccatcaac
     6301 aatgctttta gcagatacag catctccgat aacgacctgt ttcactttaa caagaagatg
     6361 ttcgccaggc ggagaatcct gcttaaaaag aaccgccaca agagagccgg ccacggcgcc
     6421 aagaacaagc tcaaacctat caccatcctg accgagaaga gcgagcggtt ccggaaaaag
     6481 ctgatcgaga gatgggcctg tgaaatcgcc gacttcttca tcaaaaataa ggtgggcaca
     6541 gtgcagatgg aaaacctgga aggcatgaag agaaaggaag atagctattt caacatccgg
     6601 ctgagaggct tctggcctta cgccgagatg cagaacaaga tcgagttcaa gctgaagcaa
     6661 tacggcatcg agatcagaaa ggtggctcct aacaacacca gcaaaacctg tagcaagtgc
     6721 ggccatctga acaactactt caacttcgag tacagaaaga agaacaagtt cccccacttc
     6781 aagtgcgaga agtgcaactt caaggaaaac gccgcttata atgctgcgct gaatatctct
     6841 aatcctaagc tgaaatctac aaaggaggaa cccggcggtg gcggagggat ggatgctaag
     6901 tcactaactg cctggtcccg gacactggtg accttcaagg atgtatttgt ggacttcacc
     6961 agggaggagt ggaagctgct ggacactgct cagcagatcg tgtacagaaa tgtgatgctg
     7021 gagaactata agaacctggt ttccttgggt tatcagctta ctaagccaga tgtgatcctc
     7081 cggttggaga agggagaaga gcccggaagc ggatccgatt acaaagatga cgatgacaaa
     7141 gactacaagg atgatgatga taagggatcc ggcgcaacaa acttctctct gctgaaacaa
     7201 gccggagatg tcgaagagaa tcctggaccg atggtgagca agggcgagga gctgttcacc
     7261 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     7321 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     7381 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     7441 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     7501 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     7561 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     7621 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     7681 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     7741 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     7801 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     7861 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     7921 actctcggca tggacgagct gtacaagtaa acgcgttaag aattcctaga gctcgctgat
     7981 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8041 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8101 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8161 gggaggattg ggaagagaat agcaggcatg ctggggagcg gccgcaggaa cccctagtga
     8221 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     8281 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     8341 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     8401 tacgtcaaag caaccatagt
//
