LOCUS       sequence-470109-        9786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the 
            sgRNA targeting the gfp gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Golla SA, Abo-Hashesh M, Gupta D, Liu Y, Mahadevan R
  TITLE     Model-Based Optimization of a qCRISPRi Circuit for Dynamic Control 
            of Metabolic Pathways.
  JOURNAL   ACS Synth Biol. 2025 Oct 17;14(10):3890-3898. doi: 
            10.1021/acssynbio.5c00095. Epub 2025 Sep 29.
  PUBMED    41021780
REFERENCE   2  (bases 1 to 9786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5c00095"; journalName: "ACS Synth Biol"; date: 
            "2025-10-17- 17"; volume: "14"; issue: "10"; pages: "3890-3898"
COMMENT     Sequence Label: pQdCas9-sggfp
FEATURES             Location/Qualifiers
     source          1..9786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1194..1988)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1305..1324)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1915..1934
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      3468..4239
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             4240..4902
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGE*QLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     misc_RNA        complement(4981..5056)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(5077..5111)
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     CDS             complement(join(5118..6704,6705..6707,6708..9194,9195..9197,
                     9198..9224))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             9231..9242
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tgaatgattg gagttagaat aatctactat aggatcatat tttattaaat tagcgtcatc
       61 ataatattgc ctccattttt tagggtaatt atctagaatt gaaatatcag atttaaccat
      121 agaatgagga taaatgatcg cgagtaaata atattcacaa tgtaccattt tagtcatatc
      181 agataagcat tgattaatat cattattgct tctacaagct ttaattttat taattattct
      241 gtatgtgtcg tcggcattta tgtttttcat acccatctct ttatccttac ctattgtttg
      301 tcgcaagttt tgcgtgttat atatcattaa aacggtaatg gattgacatt tgattctaat
      361 aaattggatt tttgtcacac tattgtatcg ctgggaatac aattacttaa cataagcacc
      421 tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aacgcaaggg
      481 aggttggtat gactataatg ataaaaaaat cggatttttt ggcaattcca tcggaggagt
      541 ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag tgggacttag
      601 ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa tatatttatg
      661 cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca acaggtgatt
      721 atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc aaagatccta
      781 atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata aataactctg
      841 ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt agtcaaggta
      901 ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag cgtattaaag
      961 ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact aaatcggttg
     1021 tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaattaa tctagataag
     1081 ttcaggatga attctctagt ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     1141 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     1201 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     1261 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     1321 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
     1381 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     1441 tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     1501 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     1561 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     1621 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     1681 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     1741 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     1801 tcgtcctgta gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     1861 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     1921 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     1981 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcaa atcttgatcc cctgcgccat
     2041 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
     2101 gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc
     2161 tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt
     2221 gtccagatag cccagtagct gacattcatc ccaggtggca cttttcgggg aaatgtgcgc
     2281 gcccgcgttc ctgctggcgc tgggcctgtt tctggcgctg gacttcccgc tgttccgtca
     2341 gcagcttttc gcccacggcc ttgatgatcg cggcggcctt ggcctgcata tcccgattca
     2401 acggccccag ggcgtccaga acgggcttca ggcgctcccg aaggtctcgg gccgtctctt
     2461 gggcttgatc ggccttcttg cgcatctcac gcgctcctgc ggcggcctgt agggcaggct
     2521 catacccctg ccgaaccgct tttgtcagcc ggtcggccac ggcttccggc gtctcaacgc
     2581 gctttgagat tcccagcttt tcggccaatc cctgcggtgc ataggcgcgt ggctcgaccg
     2641 cttgcgggct gatggtgacg tggcccactg gtggccgctc cagggcctcg tagaacgcct
     2701 gaatgcgcgt gtgacgtgcc ttgctgccct cgatgccccg ttgcagccct agatcggcca
     2761 cagcagccgc aaacgtggtc tggtcgcggg tcatctgcgc tttgttgccg atgaactcct
     2821 tggccgacag cctgccgtcc tgcgtcagcg gcaccacgaa cgcggtcatg tgcgggctgg
     2881 tttcgtcacg gtggatgctg gccgtcacga tgcgatccgc cccgtacttg tccgccagcc
     2941 acttgtgcgc cttctcgaag aacgccgcct gctgttcttg gctggccgac ttccaccatt
     3001 ccgggctggc cgtcatgacg tactcgaccg ccaacacagc gtccttgcgc cgcttctctg
     3061 gcagcaactc gcgcagtcgg cccatcgctt catcggtgct gctggccgcc cagtgctcgt
     3121 tctctggcgt cctgctggcg tcagcgttgg gcgtctcgcg ctcgcggtag gcgtgcttga
     3181 gactggccgc cacgttgccc attttcgcca gcttcttgca tcgcatgatc gcgtatgccg
     3241 ccatgcctgc ccctcccttt tggtgtccaa ccggctcgac gggggcagcg caaggcggtg
     3301 cctccggcgg gccactcaat gcttgagtat actcactaga ctttgcttcg caaagtcgtg
     3361 accgcctacg gcggctgcgg cgccctacgg gcttgctctc cgggcttcgc cctgcgcggt
     3421 cgctgcgctc ccttgccagc ccgtggatat gtggacgatg gccgcgagcg gccaccggct
     3481 ggctcgcttc gctcggcccg tggacaaccc tgctggacaa gctgatggac aggctgcgcc
     3541 tgcccacgag cttgaccaca gggattgccc accggctacc cagccttcga ccacataccc
     3601 accggctcca actgcgcggc ctgcggcctt gccccatcaa tttttttaat tttctctggg
     3661 gaaaagcctc cggcctgcgg cctgcgcgct tcgcttgccg gttggacacc aagtggaagg
     3721 cgggtcaagg ctcgcgcagc gaccgcgcag cggcttggcc ttgacgcgcc tggaacgacc
     3781 caagcctatg cgagtggggg cagtcgaagg gcgaagcccg cccgcctgcc ccccgagcct
     3841 cacggcggcg agtgcggggg ttccaagggg gcagcgccac cttgggcaag gccgaaggcc
     3901 gcgcagtcga tcaacaagcc ccggaggggc cactttttgc cggaggggga gccgcgccga
     3961 aggcgtgggg gaaccccgca ggggtgccct tctttgggca ccaaagaact agatataggg
     4021 cgaaatgcga aagacttaaa aatcaacaac ttaaaaaagg ggggtacgca acagctcatt
     4081 gcggcacccc ccgcaatagc tcattgcgta ggttaaagaa aatctgtaat tgactgccac
     4141 ttttacgcaa cgcataattg ttgtcgcgct gccaaaaagt tgcagctgat tgcgcatggt
     4201 gccgcaaccg tgcggcaccc ctaccgcatg gagataagca tggccacgca gtccagagaa
     4261 atcggcattc aagccaagaa caagcccggt cactgggtgc aaacggaacg caaagcgcat
     4321 gaggcgtggg ccgggcttat tgcgaggaaa cccacggcgg caatgctgct gcatcacctc
     4381 gtggcgcaga tgggccacca gaacgccgtg gtggtcagcc agaagacact ttccaagctc
     4441 atcggacgtt ctttgcggac ggtccaatac gcagtcaagg acttggtggc cgagcgctgg
     4501 atctccgtcg tgaagctcaa cggccccggc accgtgtcgg cctacgtggt caatgaccgc
     4561 gtggcgtggg gccagccccg cgaccagttg cgcctgtcgg tgttcagtgc cgccgtggtg
     4621 gttgatcacg acgaccagga cgaatcgctg ttggggcatg gcgacctgcg ccgcatcccg
     4681 accctgtatc cgggcgagta gcaactaccg accggccccg gcgaggagcc gcccagccag
     4741 cccggcattc cgggcatgga accagacctg ccagccttga ccgaaacgga ggaatgggaa
     4801 cggcgcgggc agcagcgcct gccgatgccc gatgagccgt gttttctgga cgatggcgag
     4861 ccgttggagc cgccgacacg ggtcacgctg ccgcgccggt agcacttggg ttgcgcagca
     4921 acccgtaagt gcgctgttcc agactatact agactgcagg tcctgaagtt aacaaaaaaa
     4981 gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta
     5041 tttctagctc taaaacgtac ttccagggct cgagcaacta gtattatacc taggactgag
     5101 ctagctgtca actcgagtta gtcacctcct agctgactca aatcaatgcg tgtttcataa
     5161 agaccagtga tggattgatg gataagagtg gcatctaaaa cttcttttgt agacgtatat
     5221 cgtttacgat caattgttgt atcaaaatat ttaaaagcag cgggagctcc aagattcgtc
     5281 aacgtaaata aatgaataat attttctgct tgttcacgta ttggtttgtc tctatgtttg
     5341 ttatatgcac taagaacttt atctaaattg gcatctgcta aaataacacg cttagaaaat
     5401 tcactgattt gctcaataat ctcatctaaa taatgcttat gctgctccac aaacaattgt
     5461 ttttgttcgt tatcttctgg actacccttc aacttttcat aatgactagc taaatataaa
     5521 aaattcacat atttgcttgg cagagccagc tcatttcctt tttgtaattc tccggcacta
     5581 gccagcatcc gtttacgacc gttttctaac tcaaaaagac tatatttagg tagtttaatg
     5641 attaagtctt ttttaacttc cttatatcct ttagcttcta aaaagtcaat cggatttttt
     5701 tcaaaggaac ttctttccat aattgtgatc cctagtaact ctttaacgga ttttaacttc
     5761 ttcgatttcc ctttttccac cttagcaacc actaggactg aataagctac cgttggacta
     5821 tcaaaaccac catatttttt tggatcccag tcttttttac gagcaataag cttgtccgaa
     5881 tttctttttg gtaaaattga ctccttggag aatccgcctg tctgtacttc tgttttcttg
     5941 acaatattga cttggggcat ggacaatact ttgcgcactg tggcaaaatc tcgcccttta
     6001 tcccagacaa tttctccagt ttccccatta gtttcgatta gagggcgttt gcgaatctct
     6061 ccatttgcaa gtgtaatttc tgttttgaag aagttcatga tattagagta aaagaaatat
     6121 tttgcggttg ctttgcctat ttcttgctca gacttagcaa tcattttacg aacatcataa
     6181 actttataat caccatagac aaactccgat tcaagttttg gatatttctt aatcaaagca
     6241 gttccaacga cggcatttag atacgcatca tgggcatgat ggtaattgtt aatctcacgt
     6301 actttataga attggaaatc ttttcggaag tcagaaacta atttagattt taaggtaatc
     6361 actttaacct ctcgaataag tttatcattt tcatcgtatt tagtattcat gcgactatcc
     6421 aaaatttgtg ccacatgctt agtgatttgg cgagtttcaa ccaattggcg tttgataaaa
     6481 ccagctttat caagttcact caaacctcca cgttcagctt tcgttaaatt atcaaactta
     6541 cgttgagtga ttaacttggc gtttagaagt tgtctccaat agtttttcat ctttttgact
     6601 acttcttcac ttggaacgtt atccgattta ccacgatttt tatcagaacg cgttaagacc
     6661 ttattgtcta ttgaatcgtc tttaaggaaa ctttgtggaa caatggcatc gacatcataa
     6721 tcacttaaac gattaatatc taattcttgg tccacataca tgtctcttcc attttggaga
     6781 taatagagat agagcttttc attttgcaat tgagtatttt caacaggatg ctctttaaga
     6841 atctgacttc ctaattcttt gataccttct tcgattcgtt tcatacgctc tcgcgaattt
     6901 ttctggccct tttgagttgt ctgattttca cgtgccattt caataacgat attttctggc
     6961 ttatgccgcc ccattacttt gaccaattca tcaacaactt ttacagtctg taaaatacct
     7021 tttttaatag cagggctacc agctaaattt gcaatatgtt catgtaaact atcgccttgt
     7081 ccagacactt gtgctttttg aatgtcttct ttaaatgtca aactatcatc atggatcagc
     7141 tgcataaaat tgcgattggc aaaaccatct gatttcaaaa aatctaatat tgttttgcca
     7201 gattgcttat ccctaatacc attaatcaat tttcgagaca aacgtcccca accagtataa
     7261 cggcgacgtt taagctgttt catcacctta tcatcaaaga ggtgagcata tgttttaagt
     7321 ctttcctcaa tcatctccct atcttcaaat aaggtcaatg ttaaaacaat atcctctaag
     7381 atatcttcat tttcttcatt atccaaaaaa tctttatctt taataatttt tagcaaatca
     7441 tggtaggtac ctaatgaagc attaaatcta tcttcaactc ctgaaatttc aacactatca
     7501 aaacattcta tttttttgaa ataatcttct tttaattgct taacggttac ttttcgattt
     7561 gttttgaaga gtaaatcaac aatggctttc ttctgttcac ctgaaagaaa tgctggtttt
     7621 cgcattcctt cagtaacata tttgaccttt gtcaattcgt tataaaccgt aaaatactca
     7681 taaagcaaac tatgttttgg tagtactttt tcatttggaa gatttttatc aaagtttgtc
     7741 atgcgttcaa taaatgattg agctgaagca cctttatcga caacttcttc aaaattccat
     7801 ggggtaattg tttcttcaga cttccgagtc atccatgcaa aacgactatt gccacgcgcc
     7861 aatggaccaa cataataagg aattcgaaaa gtcaagattt tttcaatctt ctcacgattg
     7921 tcttttaaaa atggataaaa gtcttcttgt cttctcaaaa tagcatgcag ctcacccaag
     7981 tgaatttgat ggggaataga gccgttgtca aaggtccgtt gcttgcgcag caaatcttca
     8041 cgatttagtt tcaccaataa ttcctcagta ccatccattt tttctaaaat tggtttgata
     8101 aatttataaa attcttcttg gctagctccc ccatcaatat aacctgcata tccgtttttt
     8161 gattgatcaa aaaagatttc tttatacttt tctggaagtt gttgtcgaac taaagctttt
     8221 aaaagagtca agtcttgatg atgttcatcg tagcgtttaa tcattgaagc tgatagggga
     8281 gccttagtta tttcagtatt tactcttagg atatctgaaa gtaaaatagc atctgataaa
     8341 ttcttagctg ccaaaaacaa atcagcatat tgatctccaa tttgcgccaa taaattatct
     8401 aaatcatcat cgtaagtatc ttttgaaagc tgtaatttag catcttctgc caaatcaaaa
     8461 tttgatttaa aattaggggt caaacccaat gacaaagcaa tgagattccc aaataagcca
     8521 tttttcttct caccggggag ctgagcaatg agattttcta atcgtcttga tttactcaat
     8581 cgtgcagaaa gaatcgcttt agcatctact ccacttgcgt taatagggtt ttcttcaaat
     8641 aattgattgt aggtttgtac caactggata aatagtttgt ccacatcact attatcagga
     8701 tttaaatctc cctcaatcaa aaaatgacca cgaaacttaa tcatatgcgc taaggccaaa
     8761 tagattaagc gcaaatccgc tttatcagta gaatctacca atttttttcg cagatgatag
     8821 atagttggat atttctcatg ataagcaact tcatctacta tatttccaaa aataggatga
     8881 cgttcatgct tcttgtcttc ttccaccaaa aaagactctt caagtcgatg aaagaaacta
     8941 tcatctactt tcgccatctc atttgaaaaa atctcctgta gataacaaat acgattcttc
     9001 cgacgtgtat accttctacg agctgtccgt ttgagacgag tcgcttccgc tgtctctcca
     9061 ctgtcaaata aaagagcccc tataagattt tttttgatac tgtggcggtc tgtatttccc
     9121 agaaccttga actttttaga cggaacctta tattcatcag tgatcaccgc ccatccgacg
     9181 ctatttgtgc cgatagctaa gcctattgag tatttcttat ccatagatcc tttctcctct
     9241 tttttattcg actataacaa accattttct tgcgtaaacc tgtacgatcc tacaggtaga
     9301 tctttaattt ttaaagtatg ggcaatcaat tgctcctgtt aaaattgctt tagaaatact
     9361 ttggcagcgg tttgttgtat tgagtttcat ttgcgcattg gttaaatgga aagtgacagt
     9421 acgctcactg cagcctaata tttttgaaat atcccaagag ctttttcctt cgcatgccca
     9481 cgctaaacat tctttttctc ttttggttaa atcgttgttt gatttattat ttgctatatt
     9541 tatttttcga taattatcaa ctagagaagg aacaattaat ggtatgttca tacacgcatg
     9601 taaaaataaa ctatctatat agttgtcttt ttctgaatgt gcaaaactaa gcattccgaa
     9661 gccattgtta gccgtatgaa tagggaaact aaacccagtg ataagacctg atgttttcgc
     9721 ttctttaatt acatttggag attttttatt tacagcattg ttttcaaata tattccaatt
     9781 aattgg
//
