LOCUS       sequence-470120-        1124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FabG [3-oxoacyl-[acyl-carrier-protein] reductase] from 
            Yersinia pestis with His tag in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1124)
  TITLE     Josh Beckham Lab plasmids 2025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: YpFabG_pET-28a(+)-TEV
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             29..51
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             98..118
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             863..880
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(933..951)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      947..994
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gggnggcgan atttccctct agaataattt tgtttaactt taagaaggag atataccatg
       61 ggcagcagcc atcattcatc atcatcacag cagcggcgag aatctttatt ttcagggcca
      121 tatgtctttc gagggtaaga tcgcactggt cacaggggca tcgcgtggaa ttggacgcgc
      181 tatcgctgag ctgcttgtcg aacgtggagc gtgcgttatc gggacggcta cctcggaaaa
      241 gggtgctgaa gctatcagtg cgtaccttgg cgaaaacggg aaaggcctga tgttaaacgt
      301 agtcgatccc acatcaatcg acactgtact ggcaacaatt cgtgccgagt tcggagaagt
      361 tgacatcctt gttaataatg caggaatcac ccgtgacaac ttgcttatgc gtatgaagga
      421 tgacgaatgg caagacatta ttgatacaaa cttgacttcg gtctttcgtc tgtcaaaggc
      481 agtgatgcgt gcaatgatga agaagcgttt cggacgtatt atcactattg gaagcgtagt
      541 tgggacgatg ggtaatgcgg gtcaggttaa ttatgcagca gctaaagccg gagttattgg
      601 attctcgaaa agcttggcac gcgaggtggc ttcgcgcgga atcacagtca acgttgtggc
      661 gccaggcttt atcgagaccg atatgacgcg cactttaact gatgaccaac gtgcaggaat
      721 cttggcacag gtccccgcga accgcctggg agacgcgaaa gaaattgctt ctgcagttgc
      781 atttctggcc tctgatgagg catcgtacat ttctggagag acgttacacg tcaatggggg
      841 tatgtatatg atctagctcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      901 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      961 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
     1021 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     1081 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc cttt
//
